Development of an antibody against a 170-kDa fragment of fibroin isolated from cocoon fibres of Bombyx mori

Rajan, M. K.; Balakrishnan, Abirami; Jayaraman, Kunthala

doi:10.1016/0165-022x(92)90034-8

Cited by 8 publications

(3 citation statements)

References 15 publications

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“…It is a more time-intensive process than many of the degumming methods, requiring up to 12 h to fully degum the cocoons. 49,50 Additionally, degumming in NaOH results in a lower fiber diameter than urea and sodium carbonate but did remove more sericin than urea. 49 Though the previously mentioned degumming techniques are considered "more green", degumming with urea is still a more environmentally friendly option compared to common degumming techniques (i.e., soap, sodium carbonate).…”

Section: Silk Processing To Isolate Andmentioning

confidence: 99%

“…However, compared to sodium carbonate, NaOH requires less equipment, meaning lower energy consumption and less water. It is a more time-intensive process than many of the degumming methods, requiring up to 12 h to fully degum the cocoons. , Additionally, degumming in NaOH results in a lower fiber diameter than urea and sodium carbonate but did remove more sericin than urea …”

Section: Silk Processing To Isolate and Reconstitute Fibroin Proteinsmentioning

confidence: 99%

“…While it is considered “green” by our benchmarks, using NaOH as the dissolution solvent requires large amounts of dialysis water. The main advantages that NaOH has over other methods are that it uses low-energy equipment, can be sustainably sourced, and has fewer environmental and health hazards. ,, Dissolving silk in NaOH takes up to 24 h but is a cheaper alternative to LiBr. Additionally, NaOH outperformed calcium chloride, lithium thiocyanate, and calcium nitrate in terms of Bombyx mori fibroin fiber dissolution percent (∼70%) …”

Section: Silk Processing To Isolate and Reconstitute Fibroin Proteinsmentioning

confidence: 99%

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Silk Fibroin as a Green Material

DeBari

King

Altgold

et al. 2021

ACS Biomater. Sci. Eng.

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Silk fibroin has been explored as a suitable biomaterial due to its biocompatibility, tunable degradability, low toxicity, and mechanical properties. To harness silk fibroin’s innate properties, it is purified from native silkworm cocoons by removing proteins and debris that have the potential to cause inflammatory responses. Typically, within the purification and fabrication steps, chemical solvents, energy-intensive equipment, and large quantities of water are used to reverse engineer silk fibroin into an aqueous solution and then process into the final material format. Gentler, green methods for extraction and fabrication have been developed that reduce or remove the need for harmful chemical additives and energy-inefficient equipment while still producing mechanically robust biomaterials. This review will focus on the alternative green processing and fabrication methods that have proven useful in creating silk fibroin materials for a range of applications including consumer and medical materials.

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Section: Silk Processing To Isolate Andmentioning

confidence: 99%