Development of an Analytical Method for the Determination of Arsenic in Urine by Gas Chromatography‐mass Spectrometry for Biological Monitoring of Exposure to Inorganic Arsenic

Abstract

Interday accuracy and precision were 81.3−100.0% and 0.8−9.9%, respectively. The analytical values of SRM 2669 obtained by the proposed method were sufficiently accurate. Conclusions: The proposed method overcame the disadvantages of high-performance liquid chromatography with inductively coupled plasma mass spectrometry. It was a robust, selective and cost-effective method suitable for routine analyses and could be useful for the biological monitoring of occupational exposure to inorganic arsenic. (J Occup Health 2012; 54: 434-440)

One step derivatization with British Anti-Lewsite in combination with gas chromatography coupled to triple-quadrupole tandem mass spectrometry for the fast and selective analysis of inorganic arsenic in rice

“…iAs (III) was much more readily reacted with BAL than iAs (V) under this reaction condition (at room temperature for 30 min). The result was in good agreement with the previous reports [19,24]. It has been reported that for the derivatization of iAs (V), iAs (V) should be first reduced to iAs (III) with reducing agents (SnCl 2 and KI) [19,24].…”

“…Gas chromatographic condition for the separation iAs (III) was directly derivatized with BAL according to the previous reports [19,24]. However, iAs (V) was first reduced to iAs (III) with SnCl 2 and KI solutions.…”

“…However, iAs (V) was first reduced to iAs (III) with SnCl 2 and KI solutions. Then, it was derivatized with BAL [19,24]. The experiments were conducted to obtain the optimum GC oven temperature gradient condition for the separation of the derivatized iAs standard.…”

“…TGM and TGE, however, may not be the right choice for the derivatization of iAs due to the fast sensitivity deterioration on the gas chromatography [20,21]. BAL has been shown to produce a stable complex with iAs, producing good results for the gas chromatographic speciation of iAs [19,24]. However, BAL has been reported to react only with iAs (III) at the room temperature to give organic solvent-extractable volatile complex [19,24].…”

“…However, BAL has been reported to react only with iAs (III) at the room temperature to give organic solvent-extractable volatile complex [19,24]. Therefore, an additional step for the reduction of iAs (V) to iAs (III) with strong reducing reagents (SnCl 2 and KI) was required before derivatization with BAL for the iAs analysis by GC-MS technique [19,24]. Szostek and Aldstadt [21] observed that iAs (V) was derivatized with BAL at the 70 C for 30 min.…”