Development of an analytical method for the determination of polyphenolic compounds in vegetable origin samples by liquid chromatography and pulsed amperometric detection at a glassy carbon electrode

Natale, Anna; Nardiello, Donatella; Palermo, Carmen; Muscarella, Marilena; Quinto, Maurizio; Centonze, Diego

doi:10.1016/j.chroma.2015.09.082

Cited by 21 publications

(10 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Foodstuff and plant extracts containing phenolic compounds including lignans have been electrochemically oxidized during the determination of the phenolic content [184,185,186,187] and the antioxidant capacity [188,189]. The drug etoposide, which is a semisynthetic epipodophyllotoxin glycoside, has been oxidized electrochemically using a carbon paste working electrode, a Ag/AgCl/3 M KCl reference electrode, and a platinum wire counter electrode, with a supporting electrolyte of varying pH.…”

Section: Other Oxidation Methodsmentioning

confidence: 99%

Oxidative Transformations of Lignans

Runeberg¹,

Brusentsev²,

Rendon³

et al. 2019

Molecules

View full text Add to dashboard Cite

Numerous oxidative transformations of lignan structures have been reported in the literature. In this paper we present an overview on the current findings in the field. The focus is put on transformations targeting a specific structure, a specific reaction, or an interconversion of the lignan skeleton. Oxidative transformations related to biosynthesis, antioxidant measurements, and total syntheses are mostly excluded. Non-metal mediated as well as metal mediated oxidations are reported, and mechanisms based on hydrogen abstractions, epoxidations, hydroxylations, and radical reactions are discussed for the transformation and interconversion of lignan structures. Enzymatic oxidations, photooxidation, and electrochemical oxidations are also briefly reported.

show abstract

Section: Other Oxidation Methodsmentioning

confidence: 99%

Oxidative Transformations of Lignans

Runeberg¹,

Brusentsev²,

Rendon³

et al. 2019

Molecules

View full text Add to dashboard Cite

show abstract

“…They are very reactive and causes a number of health complications. Antioxidants have the potential to scavenge them and maintain health [5–7].…”

Section: Introductionmentioning

confidence: 99%

Isolation of bioactive compounds from Bergenia ciliata (haw.) Sternb rhizome and their antioxidant and anticholinesterase activities

Zafar

Ullah

Zahoor

et al. 2019

BMC Complement Altern Med

View full text Add to dashboard Cite

BackgroundBergenia ciliata is a medicinal plant used for the treatment of diarrhea, vomiting, fever, cough, diabetes, cancer, pulmonary disorders and wound healing.MethodsIn this study, Bergenia ciliata crude extract, subfractions, and isolated compounds were evaluated for their antioxidant and anticholinesterase potential. The free radical scavenging capacities of the extracts determined using DPPH and ABTS assays. The anticholinesterase potentials were determined using acetylcholine esterase and butyryl choline esterase enzymes. To determine the phytochemical composition, the extracts were subjected to HPLC analysis and silica gel column isolation. Based on HPLC fingerprinting results, the ethyl acetate fraction was found to have more bioactive compounds and was therefore subjected to silica gel column isolation. As a result, three compounds; pyrogallol, rutin, and morin were isolated in the pure state. The structures of the isolated compounds were elucidated using spectroscopic techniques like 1H-NMR, IR and UV-Visible.ResultsThe crude extract showed maximum anticholinesterase (acetylcholinesterase = 90.22 ± 1.15% and butyrylcholinesterase = 88.22 ± 0.71%) and free radical scavenging (87.37 ± 2.45 and 83.50 ± 0.70% respectively against DPPH and ABTS radicals) potentials. The total phenolic contents (expressed as equivalent of gallic acid; mgGAE/g) were higher in ethyl acetate fraction (80.96 ± 1.74) followed by crude extract (70.65 ± 0.86) while the flavonoid contents (expressed as quercetin equivalent; mgQE/g) and were higher in crude extract (88.40 ± 1.12) followed by n-butanol fraction (60.10 ± 1.86). The isolated bioactive compounds pyrogallol, rutin, and morin were found active against ABTS and DPPH free radicals. Amongst them, pyrogallol was more active against both free radicals. Reasonable anticholinesterase activities were recorded for pyrogallol against selected enzymes.ConclusionThe extracts and isolated compounds showed antioxidant and acetylcholinesterase inhibitory potentials. It was concluded that this plant could be helpful in the treatment of oxidative stress and neurological disorders if used in the form of extracts.

show abstract

“…A novel procedure for the determination of chlorogenic acid is of great interest for scientific and therapeutic reasons. Several methods were reported for the determination of chlorogenic acid including, liquid chromatography [2], high performance liquid chromatography with ultraviolet spectroscopy [3], high performance liquid chromatography with mass spectroscopy [4], electrochemioluminescense [5], infrared spectrometry [6], and electrochemical methods [7][8][9][10][11][12][13]. However, some of the above mentioned techniques have time-consuming steps and complexity which make them unsuitable for the accurate determination of chlorogenic acid.…”

Section: Introductionmentioning

confidence: 99%

A Boron Doped Diamond Electrode Modified with Nano‐carbon Black for the Sensitive Electrochemical Determination of Chlorogenic Acid

2019

View full text Add to dashboard Cite

An electrochemical method was developed for the sensitive determination of chlorogenic acid using a boron doped diamond electrode (BDDE) modified with nano‐carbon black (nano‐CB). The active surface areas were found to be 0.059 and 0.146 cm2 for the unmodified BDDE, and nano‐CB/BDDE, respectively. Compared with a BDDE, the nano‐CB/BDDE exhibited a well‐defined redox couple for chlorogenic acid. In addition, the plot of the peak current response changing from a square root to a linear dependence on scan rate is attributed to the transition from planar diffusion to surface behaviour. The anodic and cathodic peak separations (ΔEp) were 97 mV and 14 mV at BDDE and nano‐CB/BDDE, respectively. The decrease in ΔEp at the proposed electrode indicated that the process of chlorogenic acid was greatly accelerated. Square wave voltammetry (SWV) exhibited a dynamic range in which the current versus the concentration of chlorogenic acid were linear from 2.0×10−8 to 2.0×10−6 M with a LOD of 4.1×10−9 M (based on 3Sb/m). The nano‐CB modified BDDE provided improved electrochemical behavior, high electrocatalytic activity, high sensitivity and good reproducibility.

show abstract

Development of an analytical method for the determination of polyphenolic compounds in vegetable origin samples by liquid chromatography and pulsed amperometric detection at a glassy carbon electrode

Cited by 21 publications

References 36 publications

Oxidative Transformations of Lignans

Oxidative Transformations of Lignans

Isolation of bioactive compounds from Bergenia ciliata (haw.) Sternb rhizome and their antioxidant and anticholinesterase activities

A Boron Doped Diamond Electrode Modified with Nano‐carbon Black for the Sensitive Electrochemical Determination of Chlorogenic Acid

Contact Info

Product

Resources

About