Development of an analytical method for determination of polyphenols and total tannins from leaves of Syzygium cumini L. Skeels

Abstract

Syzygium cumini L. Skeels belongs to Myrtaceae family. This species has been recognized by its antidiabetic, anti-infl ammatory, and antimicrobial activities. Despite ever-increasing scientifi c interest for this species there is no pharmacopeia method for characterization and standardization of S. cumini yet. So, toward this aim, the objective of this work was to develop an effi cient analytical methodology able to determine polyphenols and tannins content from leaves hydroethanolic extract of S. cumini using Folin-Ciocalteu method by ultraviolet absorption spectrophotometry (UV-Vis). The analytical methodology was developed for the fi rst time in the literature for leaves of this specie shown to be fast and low-cost with results expressed through tannic acid equivalent (TAE). Moreover, the methodology presented selectivity with maximum absorption at 706 nm wavelength, linearity with R 2 >0.99; limit of detection 0.275 µg TAE mL -1 and 0.102 µg TAE mL -1 ; limit of quantifi cation 1.046 µg TAE mL -1 and 0.912 µg TAE mL -1 for total polyphenols and total tannins, respectively. Furthermore, the methodology was accurate with recover value greater than 98%, as well as exact, reproductive, and robust with coeffi cient of variation values less than 15% for both compounds. All the results are found within the fi xed limits according to RDC 166/2017.

Chemical and Antioxidant Profile of Hydroalcoholic Extracts of Stachys Officinalis L., Stachys Palustris L., Stachys Sylvatica L. from Romania

“…The difference between the total level of polyphenols and polyphenols residuals represents the TTC expressed in mg tannic acid equivalents /g dry weight (mg TAE/g DW). 25,26…”