Development of an amperometric enzyme biosensor for the determination of the antioxidant <i>tert</i>‐butylhydroxyanisole in a medium of reversed micelles

Ruiz, M. Pilar; Reviejo, A.J.; Parrado, Concepción; Pingarrón, José M.

doi:10.1002/elan.1140080606

Cited by 13 publications

(5 citation statements)

References 12 publications

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“…Another alternative is to monitor the reaction of 16-ArN 2 + with an antioxidant electrochemically. Following phenolic antioxidant reactions electrochemically is now routine (47), including in reverse micelles (48) and TBHQ in emulsions (49), and loss of arenediazonium ion has been monitored electrochemically (50)(51)(52), including the reaction of 16-ArN 2 + with TBHQ in octane/H 2 O/C 12 E 6 emulsions (unpublished results). Electrochemical methods also permit continuous monitoring of reactions that are substantially faster than those monitored by the sampling technique and rapid analysis of the data to obtain k obs .…”

Section: Discussionmentioning

confidence: 99%

Kinetic Method for Determining Antioxidant Distributions in Model Food Emulsions: Distribution Constants of t-Butylhydroquinone in Mixtures of Octane, Water, and a Nonionic Emulsifier

Romsted¹,

Zhang

2002

J. Agric. Food Chem.

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The absence of reliable estimates of distributions of antioxidants in food emulsions hinders the development of a useful method for comparing the efficiencies of antioxidants. Here we describe the application of a pseudophase kinetic model, originally developed for homogeneous microemulsions, to the determination of distribution constants of tert-butylhydroquinone, TBHQ, in a fluid, opaque, model food emulsion composed of the nonionic emulsifier C(12)E(6), octane, and water. This kinetic method should be applicable to a wide variety of charged and uncharged antioxidants in emulsions composed of charged and uncharged emulsifiers. The distribution constants for partitioning of TBHQ between the oil and surfactant film regions, K(O)(I), and the aqueous and surfactant film regions, K(W)(I), were obtained by fitting changes in first-order rate constants, k(obs), with emulsifier volume fraction for the reaction of 4-hexadecyl-2,6-dimethylbenzenediazonium ion, 16-ArN(2)(+), with TBHQ. The rate of formation of the reduced arene product hexadecyl-2,6-dimethylbenzene, 16-ArH, was followed by HPLC. About 90% of the TBHQ is in the surfactant film at about 2% volume fraction of C(12)E(6), which suggests that this region may be the primary site of antioxidant activity for neutral phenolic antioxidants.

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Section: Discussionmentioning

confidence: 99%

Kinetic Method for Determining Antioxidant Distributions in Model Food Emulsions: Distribution Constants of t-Butylhydroquinone in Mixtures of Octane, Water, and a Nonionic Emulsifier

Romsted¹,

Zhang

2002

J. Agric. Food Chem.

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“…Electrochemical methods for the determination of antioxidant activity based on the application of biosensors (Ruiz et al 1996) to measure antioxidant properties of tert-butylhydroxyanisole and the cyclic voltammetry (Chevion et al 1997) to study the efficiency of some low molecular weight antioxidants are reported. DPPH method has been used to evaluate the antioxidant ability of phenol compounds (Sanchez-Moreno et al 1998).…”

Section: Developments In Dpph Methodsmentioning

confidence: 99%

Genesis and development of DPPH method of antioxidant assay

2011

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α, α-diphenyl-β-picrylhydrazyl (DPPH) free radical scavenging method offers the first approach for evaluating the antioxidant potential of a compound, an extract or other biological sources. This is the simplest method, wherein the prospective compound or extract is mixed with DPPH solution and absorbance is recorded after a defined period. However, with the advancement and sophistication in instrumental techniques, the method has undergone various modifications to suit the requirements, even though the basic approach remains same in all of them. This article presents a critical review on various developments to the DPPH method.

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“…There were also reports of electrochemical methods for the measurement of antioxidant activity which are based on biosensors using cyclic voltammetry as a detection technique [ 106 ] to measure the antioxidant capacity of tert-butyl-hydroxyanisole [ 107 ]. These were used to study the efficiency of some antioxidants with a low molecular weight.…”

Section: Chemical Tests Determining Antioxidant Activitymentioning

confidence: 99%

Analytical Methods Used in Determining Antioxidant Activity: A Review

Munteanu

Apetrei

2021

IJMS

907

527

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The study of antioxidants and their implications in various fields, from food engineering to medicine and pharmacy, is of major interest to the scientific community. The present paper is a critical presentation of the most important tests used to determine the antioxidant activity, detection mechanism, applicability, advantages and disadvantages of these methods. Out of the tests based on the transfer of a hydrogen atom, the following were presented: the Oxygen Radical Absorption Capacity (ORAC) test, the Hydroxyl Radical Antioxidant Capacity (HORAC) test, the Total Peroxyl Radical Trapping Antioxidant Parameter (TRAP) test, and the Total Oxyradical Scavenging Capacity (TOSC) test. The tests based on the transfer of one electron include the Cupric Reducing Antioxidant Power (CUPRAC) test, the Ferric Reducing Antioxidant Power (FRAP) test, the Folin–Ciocalteu test. Mixed tests, including the transfer of both a hydrogen atom and an electron, include the 2,2′-Azinobis-(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) test, and the [2,2-di(4-tert-octylphenyl)-1 -picrylhydrazyl] (DPPH) test. All these assays are based on chemical reactions and assessing the kinetics or reaching the equilibrium state relies on spectrophotometry, presupposing the occurrence of characteristic colours or the discolouration of the solutions to be analysed, which are processes monitored by specific wavelength adsorption. These assays were successfully applied in antioxidant analysis or the determination of the antioxidant capacity of complex samples. As a complementary method in such studies, one may use methods based on electrochemical (bio)sensors, requiring stages of calibration and validation. The use of chemical methods together with electrochemical methods may result in clarification of the operating mechanisms and kinetics of the processes involving several antioxidants.

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Development of an amperometric enzyme biosensor for the determination of the antioxidant tert‐butylhydroxyanisole in a medium of reversed micelles

Cited by 13 publications

References 12 publications

Kinetic Method for Determining Antioxidant Distributions in Model Food Emulsions: Distribution Constants of t-Butylhydroquinone in Mixtures of Octane, Water, and a Nonionic Emulsifier

Kinetic Method for Determining Antioxidant Distributions in Model Food Emulsions: Distribution Constants of t-Butylhydroquinone in Mixtures of Octane, Water, and a Nonionic Emulsifier

Genesis and development of DPPH method of antioxidant assay

Analytical Methods Used in Determining Antioxidant Activity: A Review

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