Development of an Amperometric Biosensor and Performance Studies with a Minimum Background Current

Abstract

An amperometric glucose biosensor with immobilized glucose oxidase enzyme is developed and its performance is investigated for the analysis of glucose concentration. A systematic method to find the background current in an amperometric biosensor is proposed, and the procedure for the minimization of the current has been reported. Tests were conducted for the analysis of glucose in different fruit juices. The current generated in an enzyme based amperometric biosensor depends on both the sensing current and the background current. The performance of amperometric biosensor is better when it is operated at optimized operating conditions and with minimum background current. The effect of current caused by the interference contained in the physiological fluids (uric acid and ascorbic acid) is found negligible. It has been found that the thickness of the membrane will affect the response time and the increase in background current will affect the sensitivity.