Development of an allergy test model: activation of human mast cells with potentially allergenic substances

Gospos, Annette; Dreikhausen, Ursula; Dartsch, Dorothee C.; Szamel, Márta; Hockertz, Stefan

doi:10.1016/s0300-483x(01)00444-9

Cited by 5 publications

(3 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Human allergens may be identified retrospectively by the identification of allergen-specific IgE. An in vitro test has been developed that identifies Dermatophagoides pteronyssinus-specific IgE in human blood by measuring histamine release by cultured human mast cells (Gospos et al, 2001).…”

Section: Discussionmentioning

confidence: 99%

Assessment of potency of allergenic activity of low molecular weight compounds based on IL-1α and IL-18 production by a murine and human keratinocyte cell line

et al. 2005

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 99%

Assessment of potency of allergenic activity of low molecular weight compounds based on IL-1α and IL-18 production by a murine and human keratinocyte cell line

et al. 2005

View full text Add to dashboard Cite

“…Studies also show that further amplifications of chronic allergic reactions may be mediated by histamine-releasing factors [99,100]. Cross-linking of mast-cell-bound IgE with an antibody against IgE elicits both type I and latephase reactions [101][102][103][104]. The immediate and delayed phases of bronchial hypersensitivity are initiated by mast cells are shown in both humans, as well as in mouse models of asthma [105,106].…”

Section: Mast Cells In Acute and Chronic Allergic Reactionsmentioning

confidence: 99%

Deciphering the structure and function of FcεRI/mast cell axis in the regulation of allergy and anaphylaxis: a functional genomics paradigm

Manikandan

Kothandaraman

Hande

et al. 2011

Cell. Mol. Life Sci.

View full text Add to dashboard Cite

Allergy and anaphylaxis are inflammatory disorders caused by immune reactions mainly induced by immunoglobulin-E that signal through the high-affinity FcεRI receptor to release the inflammatory mediators from innate immune cells. The FcεRI/mast cell axis is potently involved in triggering various intracellular signaling molecules to induce calcium release from the internal stores, induction of transcription factors such as NF-kB, secretion of various cytokines as well as lipid mediators, and degranulation, resulting in the induction of allergy and anaphylaxis. In this review, we discuss various cellular and molecular mechanisms triggered through FcεRI/mast cell axis in allergy and anaphylaxis with a special emphasis on the functional genomics paradigm.

show abstract

“…However, for the evaluation of possible skin-sensitisation effects caused by chemical substances, animal tests are currently required. No in vitro methods have been validated for the prospective identification of skinsensitising chemicals, although methods that can be useful in basic research have been described (10). To fill this gap, an expert-system rule base consisting of 40 rules was developed to identify contact allergens (11), and was implemented with the knowledge-based expert system DEREK (12), which is able to relate chemical structure to toxicity by the computerised detection of so-called "structural alerts" describing the substructures of chemical molecules that may be responsible for the adverse health effects caused by those chemicals.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of a Rule Base for Identifying Contact Allergens by using a Regulatory Database: Comparison of Data on Chemicals Notified in the European Union with “Structural Alerts” Used in the DEREK Expert System

2002

View full text Add to dashboard Cite

To assess the suitability of the use of structural alerts to identify the skin-sensitising properties of chemicals, the 40 originally published structural alerts for the prediction of skin-sensitisation properties used by the DEREK system (which now contains 59 structural alerts), have been evaluated against a database developed in the German Federal Institute for Health Protection of Consumers and Veterinary Medicine (BgVV), which contains data submitted under the procedure for notifying new chemicals within the European Union. The evaluation of the 40 structural alerts used in DEREK revealed that eight of the 40 structural alerts for the prediction of skin-sensitising potential could be used without any further refinement. Ten structural alerts may need further specifications or refinements in order avoid false-positive predictions — proposals for refinement are discussed. Not enough substances were found within the BgVV database (containing data for more than 1000 substances) to evaluate ten of the DEREK substructures; hence, for these structural alerts, a judgement on their suitability for prediction of skin-sensitisation properties in expert systems is not possible. For 12 structural alerts, no comparative result could be obtained, because these rules did not “fire” for any of the examined chemicals. As a general result of the evaluation process, the approach of using structural alerts for the prediction of skin-sensitising properties of chemicals proved to be reliable. Proposals are given for a refinement of the structural alerts for prediction of contact allergy used in the DEREK system. In addition, advice and several preconditions are given, that apply when trying to teach a computer system to use structural alerts to predict toxicological properties.

show abstract

Development of an allergy test model: activation of human mast cells with potentially allergenic substances

Cited by 5 publications

References 13 publications

Assessment of potency of allergenic activity of low molecular weight compounds based on IL-1α and IL-18 production by a murine and human keratinocyte cell line

Assessment of potency of allergenic activity of low molecular weight compounds based on IL-1α and IL-18 production by a murine and human keratinocyte cell line

Deciphering the structure and function of FcεRI/mast cell axis in the regulation of allergy and anaphylaxis: a functional genomics paradigm

Evaluation of a Rule Base for Identifying Contact Allergens by using a Regulatory Database: Comparison of Data on Chemicals Notified in the European Union with “Structural Alerts” Used in the DEREK Expert System

Contact Info

Product

Resources

About