Development of an advanced microfluidic micropipette aspiration device for single cell mechanics studies

Lee, Lap Man; Lee, Jin Woo; Chase, Danielle L.; Gebrezgiabhier, Daniel; Liu, Allen

doi:10.1063/1.4962968

Cited by 31 publications

(22 citation statements)

References 34 publications

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“…A variation of micropipette aspiration has recently overcome a key limitation of the technique: the relatively low throughput of the experiments ($20 cells/h). Lee et al developed a new microfluidic device based on microaspiration that has greatly increased the throughput of the technique ($1000 cells/h) (67), complementing other microfluidics approaches to measure cell-deformability parameters with a high throughput (68) (Fig. 4 g).…”

Section: New Approaches: the Need For Additional Measurementsmentioning

confidence: 99%

Advances in Micropipette Aspiration: Applications in Cell Biomechanics, Models, and Extended Studies

González‐Bermúdez

Guinea

Plaza

2019

Biophysical Journal

111

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With five decades of sustained application, micropipette aspiration has enabled a wide range of biomechanical studies in the field of cell mechanics. Here, we provide an update on the use of the technique, with a focus on recent developments in the analysis of the experiments, innovative microaspiration-based approaches, and applications in a broad variety of cell types. We first recapitulate experimental variations of the technique. We then discuss analysis models focusing on important limitations of widely used biomechanical models, which underpin the urge to adopt the appropriate ones to avoid misleading conclusions. The possibilities of performing different studies on the same cell are also considered. Classical approach: Observing the cell shape during the aspiration processIn micropipette-aspiration experiments, a suction pressure DP is applied by connecting the micropipette (microcapillary) to an adjustable water reservoir (Fig. 1 a) or a pump. Cell changes (Fig. 1 c) are determined microscopically by means of image analysis (39). The suction pressure is given by the height difference between the tip of the micropipette and the top of the reservoir h and the specific weight of water rg: DP ¼ rgh. If there is a flow, the pressure drop along

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Section: New Approaches: the Need For Additional Measurementsmentioning

confidence: 99%

Advances in Micropipette Aspiration: Applications in Cell Biomechanics, Models, and Extended Studies

González‐Bermúdez

Guinea

Plaza

2019

Biophysical Journal

111

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“…Lee et al. have demonstrated the stiffening of breast cancer cells (MDA_MB‐231) after introducing Taxol using MA in as shown in Fig. .…”

Section: Characterization Processesmentioning

confidence: 99%

“…(A) Fluorescence image at different stages of aspiration (B) Young's modulus. Reprinted from with permission of AIP Publishing.…”

Section: Characterization Processesmentioning

confidence: 99%

Mechanical characterization of vesicles and cells: A review

et al. 2020

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Vesicles perform many essential functions in all living organisms. They respond like a transducer to mechanical stress in converting the applied force into mechanical and biological responses. At the same time, both biochemical and biophysical signals influence the vesicular response in bearing mechanical loads. In recent years, liposomes, artificial lipid vesicles, have gained substantial attention from the pharmaceutical industry as a prospective drug carrier which can also serve as an artificial cell-mimetic system. The ability of these vesicles to enter through pores of even smaller size makes them ideal candidates for therapeutic agents to reach the infected sites effectively. Engineering of vesicles with desired mechanical properties that can encapsulate drugs and release as required is the prime challenge in this field. This requirement has led to the modifications of the composition of the bilayer membrane by adding cholesterol, sphingomyelin, etc. In this article, we review the manufacturing and characterization techniques of various artificial/synthetic vesicles. We particularly focus on the electric field-driven characterization techniques to determine different properties of vesicle and its membranes, such as bending rigidity, viscosity, capacitance, conductance, etc., which are indicators of their content and mobility. Similarities and differences between artificial vesicles, natural vesicles, and cells are highlighted throughout the manuscript since most of these artificial vesicles are intended for cell mimetic functions.

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“…More importantly, in the present study, we observed that CuB changed the mechanical properties of breast cancer cells. It is well known that the cytoskeleton of cancer cells is deformed more easily than that of normal cells, enhancing their ease of migration through the bloodstream to distant tissues (Lee, Lee, Chase, Gebrezgiabhier, & Liu, 2016). Changes in the cytoskeleton affect the morphology and mechanical properties of cells (Kumar & Weaver, 2009b).…”

Section: Mechanism Of Cub-induced Changes In Mechanical Properties mentioning

confidence: 99%

Cucurbitacin B inhibits the migration and invasion of breast cancer cells by altering the biomechanical properties of cells

Liang

Zhang

Yuan

et al. 2018

Phytotherapy Research

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Changes in cellular biomechanical properties affect cell migration and invasion. The natural compound Cucurbitacin B (CuB) has potent anticancer activity; however, the mechanism underlying its inhibitory effect on breast cancer metastasis needs further study. Here, we showed that low‐dose CuB inhibited adhesion and altered the viscoelasticity of breast cancer cells, thereby, reducing cell deformability. In vitro and in vivo experiments proved that CuB effectively inhibited the migration and invasion of breast cancer cells. Further studies have found that CuB downregulated the expression of F‐actin/vimentin/FAK/vinculin in breast cancer cells, altering the distribution and reorganization of cytoskeletal proteins in the cells. CuB inhibited signaling by the Rho family GTPases RAC1/CDC42/RhoA downstream of integrin. These findings indicate that CuB has been proven to mediate the reorganization and distribution of cytoskeletal proteins of breast cancer cells through RAC1/CDC42/RhoA signaling, which improves the mechanical properties of cell adhesion and deformation and consequently inhibits cell migration and invasion.

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Development of an advanced microfluidic micropipette aspiration device for single cell mechanics studies

Cited by 31 publications

References 34 publications

Advances in Micropipette Aspiration: Applications in Cell Biomechanics, Models, and Extended Studies

Advances in Micropipette Aspiration: Applications in Cell Biomechanics, Models, and Extended Studies

Mechanical characterization of vesicles and cells: A review

Cucurbitacin B inhibits the migration and invasion of breast cancer cells by altering the biomechanical properties of cells

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