Development of an adhesive sheet for direct counting of bacteria on solid surfaces

Yamaguchi, Naohito; Ishidoshiro, Ai; Yoshida, Yukari; Saika, Takeshi; Senda, Shuji; Nasu, Masao

doi:10.1016/s0167-7012(02)00246-4

Cited by 29 publications

(19 citation statements)

References 18 publications

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“…These conventional sampling methods have several problems that should be resolved: use of water for swabbing methods; potential risk of injury if the handle of the swab is broken during sampling; contamination of surfaces with culture medium components of stamp agars; and difficulty of sampling from curved surfaces. For sampling microbial cells on solid surfaces, we have developed a microbe-collecting adhesive sheet 9) . This thin sheet has high operability, needs no water for sampling, and is easy to transport and store.…”

Section: Introductionmentioning

confidence: 99%

Bacterial Monitoring in the International Space Station-“Kibo” Based on rRNA Gene Sequence

Yamaguchi

Ichijo

Nasu

2016

AEROSPACE TECHNOLOGY JAPAN

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Section: Introductionmentioning

confidence: 99%

Bacterial Monitoring in the International Space Station-“Kibo” Based on rRNA Gene Sequence

Yamaguchi

Ichijo

Nasu

2016

AEROSPACE TECHNOLOGY JAPAN

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“…Lessons learned in restoring operations within large building complexes contaminated with Bacillus anthracis spores or ricin toxin have led to the development of more-efficient tools for the detection of biological pathogens and means of decontaminating affected facilities. A key step in determining the extent of contamination during the initial stages of a bioterrorist attack and in ascertaining if a building can be reoccupied in the final stages of cleanup is our ability to effectively sample an area and deliver a specimen for testing.Currently there are several commercially available sampling products (2,3,8,9,16,23), but test data demonstrate that they are limited in their ability to detect and recover biological samples, with estimated recovery efficiencies ranging from approximately 10% to 40% (8,11,19). Studies have also shown that a significant proportion of the biological agent that can be sampled from a solid surface remains adhered to the sampling materials despite efforts to wash it off for testing or is lost during the sample processing (1,7,19).…”

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confidence: 99%

Surface Sampling of Spores in Dry-Deposition Aerosols

Edmonds

Collett

Valdes

et al. 2009

Appl Environ Microbiol

108

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The ability to reliably and reproducibly sample surfaces contaminated with a biological agent is a critical step in measuring the extent of contamination and determining if decontamination steps have been successful. The recovery operations following the 2001 attacks with Bacillus anthracis spores were complicated by the fact that no standard sample collection format or decontamination procedures were established. Recovery efficiencies traditionally have been calculated based upon biological agents which were applied to test surfaces in a liquid format and then allowed to dry prior to sampling tests, which may not be best suited for a real-world event with aerosolized biological agents. In order to ascertain if differences existed between air-dried liquid deposition and biological spores which were allowed to settle on a surface in a dried format, a study was undertaken to determine if differences existed in surface sampling recovery efficiencies for four representative surfaces. Studies were then undertaken to compare sampling efficiencies between liquid spore deposition and aerosolized spores which were allowed to gradually settle under gravity on four different test coupon types. Tests with both types of deposition compared efficiencies of four unique swabbing materials applied to four surfaces with various surface properties. Our studies demonstrate that recovery of liquid-deposited spores differs significantly from recovery of dry aerosol-deposited spores in most instances. Whether the recovery of liquid-deposited spores is overexaggerated or underrepresented with respect to that of aerosol-deposited spores depends upon the surface material being tested.Since 2001, the biological defense community has made great strides in improving our readiness to respond to and recover from an attack with a biological pathogen. Lessons learned in restoring operations within large building complexes contaminated with Bacillus anthracis spores or ricin toxin have led to the development of more-efficient tools for the detection of biological pathogens and means of decontaminating affected facilities. A key step in determining the extent of contamination during the initial stages of a bioterrorist attack and in ascertaining if a building can be reoccupied in the final stages of cleanup is our ability to effectively sample an area and deliver a specimen for testing.Currently there are several commercially available sampling products (2,3,8,9,16,23), but test data demonstrate that they are limited in their ability to detect and recover biological samples, with estimated recovery efficiencies ranging from approximately 10% to 40% (8,11,19). Studies have also shown that a significant proportion of the biological agent that can be sampled from a solid surface remains adhered to the sampling materials despite efforts to wash it off for testing or is lost during the sample processing (1,7,19).Historically, recovery efficiencies of swabs found in sampling kits have been calculated based on their ability to recover a liquid bacterial...

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“…Yan et al (44) also obtained better recoveries of L. monocytogenes from food contact surfaces with premoistened one-ply composite tissues than with the sponge. Another study found that adhesive tape recovered more bacterial cells from solid surfaces than the cotton swab, suggesting that it could be used for routine monitoring of environmental quality (43).…”

Section: Resultsmentioning

confidence: 99%

Comparison of Sampling Procedures for Recovery of Listeria monocytogenes from Stainless Steel Food Contact Surfaces

Gómez

Ariño

Carramiñana

et al. 2012

Journal of Food Protection

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A number of techniques exist for microbiological sampling of food processing environments in food industries. In the present study the efficacies of nine sampling procedures for the recovery of Listeria monocytogenes from food contact surfaces, including a new sampling device consisting of a miniroller, were evaluated and compared. A stainless steel table was inoculated with L. monocytogenes strain 935 (serovar 4b, human origin) and L. monocytogenes strain 437/07 (serovar 1/2b, food origin), at 105 CFU/100 cm2. L. monocytogenes strain 935 was best recovered with the minirollers (recovery of up to 6.27%), while poor recoveries (<0.30%) were obtained with the towel (one-ply composite tissue), alginate swab, metallic swab, and Petrifilm methods. In the case of L. monocytogenes strain 437/07 the replicate organism detection and counting (RODAC) ALOA contact plates yielded the best recoveries (4.15%), followed by the minirollers (up to 1.52%). Overall, recovery percentages with the minirollers were higher with stomacher homogenization than with Vibromatic agitation. The recovery percentages obtained for the Listeria strain of human origin were higher than those obtained with the food strain for all sampling procedures except Petrifilm and RODAC ALOA. With the miniroller device coated with wool fiber, the recovery of L. monocytogenes can be improved from 2 to 17 times over recoveries obtained with the sponge and cotton swab. This is the first report of a miniroller device for microbiological sampling in the available literature. The novel sampling procedure is convenient to apply on surfaces, is cost-effective, and results in better recovery of L. monocytogenes than do the conventional methods.

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Development of an adhesive sheet for direct counting of bacteria on solid surfaces

Cited by 29 publications

References 18 publications

Bacterial Monitoring in the International Space Station-“Kibo” Based on rRNA Gene Sequence

Bacterial Monitoring in the International Space Station-“Kibo” Based on rRNA Gene Sequence

Surface Sampling of Spores in Dry-Deposition Aerosols

Comparison of Sampling Procedures for Recovery of Listeria monocytogenes from Stainless Steel Food Contact Surfaces

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Development of an adhesive sheet for direct counting of bacteria on solid surfaces

Cited by 29 publications

References 18 publications

Bacterial Monitoring in the International Space Station-&ldquo;Kibo&rdquo; Based on rRNA Gene Sequence

Bacterial Monitoring in the International Space Station-&ldquo;Kibo&rdquo; Based on rRNA Gene Sequence

Surface Sampling of Spores in Dry-Deposition Aerosols

Comparison of Sampling Procedures for Recovery of Listeria monocytogenes from Stainless Steel Food Contact Surfaces

Contact Info

Product

Resources

About

Bacterial Monitoring in the International Space Station-“Kibo” Based on rRNA Gene Sequence

Bacterial Monitoring in the International Space Station-“Kibo” Based on rRNA Gene Sequence