2015
DOI: 10.1016/j.chroma.2015.05.072
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Development of an accurate and high-throughput methodology for structural comprehension of chlorophylls derivatives. (I) Phytylated derivatives

Abstract: Phytylated chlorophyll derivatives undergo specific oxidative reactions through the natural metabolism or during food processing or storage, and consequently pyro-, 13(2)-hydroxy-, 15(1)-hydroxy-lactone chlorophylls, and pheophytins (a and b) are originated. New analytical procedures have been developed here to reproduce controlled oxidation reactions that specifically, and in reasonable amounts, produce those natural target standards. At the same time and under the same conditions, 16 natural chlorophyll deri… Show more

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Cited by 47 publications
(59 citation statements)
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(57 reference statements)
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“…These products were identified according to their chromatographic behavior (retention time), UV‐Visible spectra, and MS high‐accuracy measurements, including mass error, isotopic pattern, and the featured product ions. These characteristic data, presented in Table S1, Supporting Information, were compared with those of pure standards and data available in the literature . However, the identification of phytyl‐chlorin e 6 was supported by the chromatographic behavior and features of the UV‐visible spectrum in comparison with its de‐esterified counterpart chlorin e 6 because this is the commercial standard available .…”
Section: Resultsmentioning
confidence: 99%
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“…These products were identified according to their chromatographic behavior (retention time), UV‐Visible spectra, and MS high‐accuracy measurements, including mass error, isotopic pattern, and the featured product ions. These characteristic data, presented in Table S1, Supporting Information, were compared with those of pure standards and data available in the literature . However, the identification of phytyl‐chlorin e 6 was supported by the chromatographic behavior and features of the UV‐visible spectrum in comparison with its de‐esterified counterpart chlorin e 6 because this is the commercial standard available .…”
Section: Resultsmentioning
confidence: 99%
“…Standard of chlorophyll and pheophorbide a were purchased from Wako (Neuss, Germany) and chlorin e 6 from Frontier Scientific (Logan, USA). The other chlorophyll derivatives were obtained following previously detailed the protocols . Commercial Spirulina (NaturGreen) was purchased from a local store.…”
Section: Methodsmentioning
confidence: 99%
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