Development of Amperometric Glucose Biosensor Based on Prussian Blue Functionlized TiO2 Nanotube Arrays

Abstract

Amperometric biosensors consisting of oxidase and peroxidase have attracted great attention because of their wide application. The current work demonstrates a novel approach to construct an enzymatic biosensor based on TiO2 nanotube arrays (TiNTs) as a supporting electrode on which Prussian Blue (PB)-an “artificial enzyme peroxidase” and enzyme glucose oxidase (GOx) have been immobilized. For this, PB nanocrystals are deposited onto the nanotube wall photocatalytically using the intrinsic photocatalytical property of TiO2, and the GOx/AuNPs nanobiocomposites are subsequently immobilized into the nanotubes via the electrodeposition of polymer. The resulting electrode exhibits a fast response, wide linear range, and good stability for glucose sensing. The sensitivity of the sensor is as high as 248 mA M−1 cm−2, and the detection limit is about 3.2 μM. These findings demonstrate a promising strategy to integrate enzymes and TiNTs, which could provide an analytical access to a large group of enzymes for bioelectrochemical applications including biosensors and biofuel cells.

The Influence of the Parameters of a Gold Nanoparticle Deposition Method on Titanium Dioxide Nanotubes, Their Electrochemical Response, and Protein Adsorption

“…In recent decades, electrochemical biosensors have been an active research field, attracting considerable attention as potential successors to a wide range of analytical techniques with rapid response and high selectivity [1,2]. Titanium dioxide nanotube arrays have demonstrated a number of important applications, including biosensors for the detection of interleukin-6 [3] or glucose [4]. Titanium dioxide nanotube (TNT) structures can be produced through the anodization of titanium foil [5].…”

Precise Construction and Tuning of an Aerolysin Single-Biomolecule Interface for Single-Molecule Sensing

“…The interface of a sensor, where the interactions between reactive groups and analytes produce readable signal responses that determine the sensitivity of the sensor, is the key to its performance. [1][2][3][4] Single-molecule measurements provide a more precise and deep understanding of molecular interaction, especially for heterogeneities and stochastic behaviors. To meet the requirement of precise measurement at the single-molecule level, one of the most important techniques is to precisely construct a functional single-molecule interface, whose volume is compatible with that of a single molecule.…”

Cysteine‐metal Porous Frameworks as Biosensing Elements for the Adsorption of Reactive Oxygen Species

“…Among the different biopolymer based sensors designed so far, enzyme-based sensors are dominant. [32,33] However, as the binding of enzymes with different transducers does not produce stable products and the mobilization of enzymes from the surface of transducers is difficult to tackle, designs of enzyme-based sensors are not very attractive. [34] These limitations can be overcome by replacing enzymes by suitable enzyme-free bioelements that can produce stable sensors and facilitate the same enzymatic reactions.…”