Development of amino acid derivatization reagents for liquid chromatography electrospray ionization mass spectrometric analysis and ionization efficiency measurements

Rebane, Riin; Rodima, Toomas; Kütt, Agnes; Herodes, Koit

doi:10.1016/j.chroma.2015.02.050

Cited by 30 publications

(30 citation statements)

References 27 publications

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“…One of the aims of this study was to find out whether the solvent and ion source are important factors when choosing the derivatization reagent. The information gained from the log IE measurements can help towards designing new derivatization reagents that provide higher ionization efficiency and lower detection limits . Structures of compounds with higher efficiencies can be used as the basis for new derivatization reagents.…”

Section: Resultsmentioning

confidence: 99%

“…The MS ion optics parameters were optimized only by setting the Target Mass parameter. This approach has been previously validated for this instrument …”

Section: Methodsmentioning

confidence: 99%

“…This also provides a lower limit of detection (LOD). Therefore, there is a special interest towards derivatization reagents as the information gained from the log IE scale can help towards designing new derivatization reagents for ESI and APCI …”

Section: Introductionmentioning

confidence: 99%

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Ionization efficiency ladders as tools for choosing ionization mode and solvent in liquid chromatography/mass spectrometry

Rebane

Kruve

Liigand

et al. 2019

Rapid Comm Mass Spectrometry

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Rationale The choice of mobile phase components and optimal ion source, mainly electrospray ionization (ESI) or atmospheric pressure chemical ionization (APCI), is a crucial part in liquid chromatography/mass spectrometry (LC/MS) method development to achieve higher sensitivity and lower detection limits. In this study we demonstrate how to rigorously solve these questions by using ionization efficiency scales. Methods Four ionization efficiency scales are used: recorded with both APCI and ESI sources and using both methanol‐ and acetonitrile‐containing mobile phases. Each scale contains altogether more than 50 compounds. In addition, measurements with a chromatographic column were also performed. Results We observed a correlation between calibration graph slopes under LC conditions and logIE values in ESI (but not APCI) thereby validating the use of logIE values for choosing the ion source. Most of the studied compounds preferred ESI as an ion source and methanol as mobile organic phase. APCI remains the ion source of choice for polycyclic aromatic hydrocarbons. For APCI, both acetonitrile and methanol provide similar ionization efficiencies with few exceptions. Conclusions Overall the results of this work give a concise guideline for practitioners in choosing an ion source for LC/MS analysis on the basis of the chemical nature of the analytes.

show abstract

Section: Resultsmentioning

confidence: 99%

“…The MS ion optics parameters were optimized only by setting the Target Mass parameter. This approach has been previously validated for this instrument …”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Ionization efficiency ladders as tools for choosing ionization mode and solvent in liquid chromatography/mass spectrometry

Rebane

Kruve

Liigand

et al. 2019

Rapid Comm Mass Spectrometry

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show abstract

“…The ionization efficiency (IE) of compound B 1 relative to compound B 2 is defined as the ratio of the respective response factors according to the followed equation as described in Rebane et al ., where R denotes the MS response and C denotes the concentration of the respective compounds,

IE ((),, \frac{B 1}{B 2}) = \frac{\frac{R 1}{C 1}}{\frac{R 2}{C 2}}

…”

Section: Resultsmentioning

confidence: 99%

“…Based on Lissamine rhodamine B sulfonyl chloride (LRSC) in our previous work, a new derivatization regent – 4'‐carbonyl chloride rosamine (CCR) – with a permanent charged moiety was designed and synthesized . According to the ion efficiency (IE) reported in the literature, the improvement of CCR was also discussed in this work.…”

mentioning

confidence: 99%