Development of allele-specific PCR methodology (AS-PCR) to screening A. lumbricoides and A. suum

Santos, Talita Rodrigues dos; Furtado, Luis Fernando Viana; Araujo, Andreina de Carvalho; Medeiros, Celi da Silva; Germano, Pedro Henrique Vieira; Oliveira, Valéria Nayara Gomes Mendes de; Rabelo, Élida Mara Leite

doi:10.1007/s00436-022-07572-x

Cited by 2 publications

(2 citation statements)

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“…In fact, genomic analyses of A. lumbricoides obtained from humans of a village without pig husbandry showed a high heterozygocity and hybridization of A. lumbricoides and A. suum, suggesting a potential interbred Ascaris complex infecting humans [ 44 ]. In any case, an allele-specific PCR using the ITS-1 of A. suum and A. lumbricoides showed good resolution for discriminating both ascarids, which may become useful for epidemiological and control purposes [ 45 ].…”

Section: Discussionmentioning

confidence: 99%

Mitochondrial and ribosomal markers in the identification of nematodes of clinical and veterinary importance

Mejías-Alpízar,

Porras-Silesky,

Rodríguez

et al. 2024

Parasites Vectors

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Background Nematodes of the Ascarididae, Ancylostomatidae and Onchocercidae families are parasites of human and veterinary importance causing infections with high prevalence worldwide. Molecular tools have significantly improved the diagnosis of these helminthiases, but the selection of genetic markers for PCR or metabarcoding purposes is often challenging because of the resolution these may show. Methods Nuclear 18S rRNA, internal transcribed spacers 1 (ITS-1) and 2 (ITS-2), mitochondrial gene cytochrome oxidase 1 (cox1) and mitochondrial rRNA genes 12S and 16S loci were studied for 30 species of the mentioned families. Accordingly, their phylogenetic interspecies resolution, pairwise nucleotide p-distances and sequence availability in GenBank were analyzed. Results The 18S rRNA showed the least interspecies resolution since separate species of the Ascaris, Mansonella, Toxocara or Ancylostoma genus were intermixed in phylogenetic trees as opposed to the ITS-1, ITS-2, cox1, 12S and 16S loci. Moreover, pairwise nucleotide p-distances were significantly different in the 18S compared to the other loci, with an average of 99.1 ± 0.1%, 99.8 ± 0.1% and 98.8 ± 0.9% for the Ascarididae, Ancylostomatidae and Onchocercidae families, respectively. However, ITS-1 and ITS-2 average pairwise nucleotide p-distances in the three families ranged from 72.7% to 87.3%, and the cox1, 12S and 16S ranged from 86.4% to 90.4%. Additionally, 2491 cox1 sequences were retrieved from the 30 analyzed species in GenBank, whereas 212, 1082, 994, 428 and 143 sequences could be obtained from the 18S, ITS-1, ITS-2, 12S and 16S markers, respectively. Conclusions The use of the cox1 gene is recommended because of the high interspecies resolution and the large number of sequences available in databases. Importantly, confirmation of the identity of an unknown specimen should always be complemented with the careful morphological examination of worms and the analysis of other markers used for specific parasitic groups. Graphical Abstract

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Section: Discussionmentioning

confidence: 99%

Mitochondrial and ribosomal markers in the identification of nematodes of clinical and veterinary importance

Mejías-Alpízar,

Porras-Silesky,

Rodríguez

et al. 2024

Parasites Vectors

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“…Further digital images remain available for referencing and auditing purposes. On the other hand, molecular techniques such as qPCR exhibit high sensitivity and can differentiate human and animal species based on their DNA/RNA sequences (Dos Santos et al 2022 ; Furtado et al 2020 ; Ngcamphalala et al 2020 ; Palma et al 2019 ; Xu et al 2021 ; Zhou et al 2021 ), significantly improving diagnostic sensitivity and offering the prospect of diagnosing even very low-level infection intensities (Benjamin-Chung et al 2020 ; Dunn et al 2020 ). This enables better monitoring of parasite infections and their response to treatment.…”

Section: One-health Approach To Anthelmintic Drug Resistancementioning

confidence: 99%

Anthelmintic resistance in soil-transmitted helminths: One-Health considerations

Ng’etich,

Amoah,

Bux

et al. 2023

Parasitol Res

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The One-Health approach recognizes the intricate connection between human, animal, and environmental health, and that cooperative effort from various professionals provides comprehensive awareness and potential solutions for issues relating to the health of people, animals, and the environment. This approach has increasingly gained appeal as the standard strategy for tackling emerging infectious diseases, most of which are zoonoses. Treatment with anthelmintics (AHs) without a doubt minimizes the severe consequences of soil-transmitted helminths (STHs); however, evidence of anthelmintic resistance (AR) development to different helminths of practically every animal species and the distinct groups of AHs is overwhelming globally. In this regard, the correlation between the application of anthelmintic drugs in both human and animal populations and the consequent development of anthelmintic resistance in STHs within the context of a One-Health framework is explored. This review provides an overview of the major human and animal STHs, treatment of the STHs, AR development and drug-related factors contributing towards AR, One-Health and STHs, and an outline of some One-Health strategies that may be used in combating AR.

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Development of allele-specific PCR methodology (AS-PCR) to screening A. lumbricoides and A. suum

Cited by 2 publications

References 56 publications

Mitochondrial and ribosomal markers in the identification of nematodes of clinical and veterinary importance

Mitochondrial and ribosomal markers in the identification of nematodes of clinical and veterinary importance

Anthelmintic resistance in soil-transmitted helminths: One-Health considerations

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