Development of Algal Systems for Hydrogen Photoproduction: Addressing the Hydrogenase Oxygen‐sensitivity Problem

Ghirardi, Maria L.; King, Paul W.; Kosourov, Sergey; Forestier, Marc; Zhang, Liping; Seibert, Michael

doi:10.1002/3527606742.ch11

Cited by 13 publications

(21 citation statements)

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“…Despite the higher than WT level of in vitro activities observed in the double mutant rescued by introduction of HYDA1 (Fig. 3), these strains show maximal in vivo H 2 -photoproduction rates that are similar to the WT, support for the conclusion that the hydrogenase enzyme level may not be the primary factor limiting in vivo H 2 photoproduction [25].…”

Section: Resultsmentioning

confidence: 60%

Genetic disruption of both Chlamydomonas reinhardtii [FeFe]-hydrogenases: Insight into the role of HYDA2 in H2 production

Meuser

D’Adamo

Jinkerson

et al. 2012

Biochemical and Biophysical Research Communications

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Chlamydomonas reinhardtii (Chlamydomonas throughout) encodes two [FeFe]-hydrogenases, designated HYDA1 and HYDA2. While HYDA1 is considered the dominant hydrogenase, the role of HYDA2 is unclear. To study the individual functions of each hydrogenase and provide a platform for future bioengineering, we isolated the Chlamydomonas hydA1-1, hydA2-1 single mutants and the hydA1-1 hydA2-1 double mutant. A reverse genetic screen was used to identify a mutant with an insertion in HYDA2, followed by mutagenesis of the hydA2-1 strain coupled with a H(2) chemosensor phenotypic screen to isolate the hydA1-1 hydA2-1 mutant. Genetic crosses of the hydA1-1 hydA2-1 mutant to wild-type cells allowed us to also isolate the single hydA1-1 mutant. Fermentative, photosynthetic, and in vitro hydrogenase activities were assayed in each of the mutant genotypes. Surprisingly, analyses of the hydA1-1 and hydA2-1 single mutants, as well as the HYDA1 and HYDA2 rescued hydA1-1 hydA2-1 mutant demonstrated that both hydrogenases are able to catalyze H(2) production from either fermentative or photosynthetic pathways. The physiology of both mutant and complemented strains indicate that the contribution of HYDA2 to H(2) photoproduction is approximately 25% that of HYDA1, which corresponds to similarly low levels of in vitro hydrogenase activity measured in the hydA1-1 mutant. Interestingly, enhanced in vitro and fermentative H(2) production activities were observed in the hydA1-1 hydA2-1 strain complemented with HYDA1, while maximal H(2)-photoproduction rates did not exceed those of wild-type cells.

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Section: Resultsmentioning

confidence: 60%

Genetic disruption of both Chlamydomonas reinhardtii [FeFe]-hydrogenases: Insight into the role of HYDA2 in H2 production

Meuser

D’Adamo

Jinkerson

et al. 2012

Biochemical and Biophysical Research Communications

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“…This would allow us to use the organisms -or the isolated enzymes -in biotechnological processes. [20][21][22][23] Furthermore, it would provide the foundation for designing biomimetic -or bioinspired -artificial catalysts for large-scale water splitting and hydrogen production in the future.…”

Section: -11mentioning

confidence: 99%

Solar water-splitting into H2 and O2: design principles of photosystem II and hydrogenases

Lubitz¹,

Reijerse²,

Messinger³

2008

Energy Environ. Sci.

399

362

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This review aims at presenting the principles of water-oxidation in photosystem II and of hydrogen production by the two major classes of hydrogenases in order to facilitate application for the design of artificial catalysts for solar fuel production. W: Lubitz E: Reijerse J: Messinger W. Lubitz is director of the MPI for Bioinorganic Chemistry and a specialist in advanced EPR techniques. He has a strong interest in both water-splitting and hydrogen production. E. Reijerse is working on hydrogenases using FTIR and EPR/ENDOR spectroscopies. J. Messinger is analyzing photosynthetic and artificial water-splitting employing O 2 measurements, mass spectrometry (MIMS), EPR and XAS.

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“…In green algae, anaerobiosis [138] inactivates the CO 2 fixation pathway that leads to downregulation of photosynthetic electron transport from water to ferredoxin (see Figure 14(a)). Consequently, anaerobic green algae show limited rates of hydrogen production, as reported under conditions of anaerobiosis [139,140]. The downregulation of photosynthesis under anaerobiosis may be a result of both the nondissipation of a proton gradient that is established during photosynthetic electron transport [141], and of the establishment of cyclic, nonproductive electron transfer around Photosystem I, the so-called state 2 [142].…”

Section: Research Approaches and Statusmentioning

confidence: 95%

Renewable hydrogen production

Turner

Sverdrup

Mann

et al. 2008

Int. J. Energy Res.

849

554

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SUMMARYThe U.S. Department of Energy and the National Renewable Energy Laboratory are developing technologies to produce hydrogen from renewable, sustainable sources. A cost goal of $2.00-$3:00 kg À1 of hydrogen has been identified as the range at which delivered hydrogen becomes cost competitive with gasoline for passenger vehicles. Electrolysis of water is a standard commercial technology for producing hydrogen. Using wind and solar resources to produce the electricity for the process creates a renewable system. Biomass-to-hydrogen processes, including gasification, pyrolysis, and fermentation, are less well-developed technologies. These processes offer the possibility of producing hydrogen from energy crops and from biomass materials such as forest residue and municipal sewage. Solar energy can be used to produce hydrogen from water and biomass by several conversion pathways. Concentrated solar energy can generate high temperatures at which thermochemical reactions can be used to split water. Photoelectrochemical water splitting and photobiology are long-term options for producing hydrogen from water using solar energy. All these technologies are in the development stage.

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Development of Algal Systems for Hydrogen Photoproduction: Addressing the Hydrogenase Oxygen‐sensitivity Problem

Cited by 13 publications

References 0 publications

Genetic disruption of both Chlamydomonas reinhardtii [FeFe]-hydrogenases: Insight into the role of HYDA2 in H2 production

Genetic disruption of both Chlamydomonas reinhardtii [FeFe]-hydrogenases: Insight into the role of HYDA2 in H2 production

Solar water-splitting into H2 and O2: design principles of photosystem II and hydrogenases

Renewable hydrogen production

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