Development of a water dispersible granule (WDG) formulation of <i>Bacillus thuringiensis</i> for the management of <i>Spodoptera litura</i> (Fab.)

Devi, P. S. Vimala; Duraimurugan, P.; Chandrika, K.; Vineela, V.

doi:10.1080/09583157.2021.1895073

Cited by 8 publications

(4 citation statements)

References 19 publications

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“…Thus, our study supports previous research and demonstrates that Btk has a high insecticidal effect against S. exigua (Moar et al 1990, Hosseinzadeh et al 2016, Park et al 2022). Furthermore, previous studies have shown the toxicity of commercial Btk (53,000 SU/mg, Delfin, WG) to S. litura , with an LC 50 of 633 g/ml (Devi et al 2021). Of the isolates, the DW strain has a high specificity to S. litura , and the LC 50 value (576.69 µg/ml) of the laboratory bioassay in this study was lower than that of commercial Btk (633 µg/ml) for S. litura (Table 6).…”

Section: Discussionmentioning

confidence: 99%

Relative activity of 15 bacterial strains against the larvae of Helicoverpa armigera, Spodoptera exigua, and Spodoptera litura (Lepidoptera: Noctuidae)

Cao

Chen

et al. 2023

Journal of Economic Entomology

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Crystal toxins produced by different strains of entomopathogenic Bacillus thuringiensis (Bt) have been characterized and widely applied as commercial biological pesticides owing to their excellent insecticidal properties. This study aimed to identify novel bacterial strains effective in controlling Spodoptera exigua Hübner, Helicoverpa armigera Hübner, and Spodoptera litura Fabricius. Fifteen culturable bacterial strains were isolated from 60 dead larvae (H. armigera and S. exigua) collected in the field. The biochemical characteristics and 16S rRNA sequences of these strains indicated that one strain (B7) was Lysinibacillus sp., 12 strains (B1, B3, B4, B5, B6, B8, P2, P3, P4, P5, P6, and DW) were Bt kurstaki, and P2-2 and B2 were Bacillus velezensis subsp. Laboratory bioassays indicated that strains B3, P6, B6, and P4 showed high toxicity to second-instar larvae of S. exigua, with LC50 values of 5.11, 6.74, 205.82, and 595.93 µg/ml, respectively; while the strains P5, B5, B6, and P6, were the most efficient against second-instar larvae of H. armigera with LC50 values of 725.82, 11,022.72, 1,282.90, 2,005.28, respectively, and strains DW, P3, P2, and B4 had high insecticidal activity against second-instar larvae of S. litura with LC50 values of 576.69, 1,660.96, 6,309.42, and 5,486.10 µg/ml, respectively. In conclusion, several Bt kurstaki strains with good toxicity potential were isolated and identified in this study. These strains are expected to be useful for biointensive integrated pest management programs to reduce the use of synthetic insecticides.

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Section: Discussionmentioning

confidence: 99%

Relative activity of 15 bacterial strains against the larvae of Helicoverpa armigera, Spodoptera exigua, and Spodoptera litura (Lepidoptera: Noctuidae)

Cao

Chen

et al. 2023

Journal of Economic Entomology

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“…To develop and formulate these products, Trichoderma isolates were cultured in PDB for 7-10 days, at 150 rpm and 25°C in an orbital shaker (Optima shaker OS-72, New Delhi, India). The broth was filtered via muslin cloth to eliminate the Trichoderma biomass, and the filtrate used to provide the active ingredients for the formulation of biofungicides (Mulatu et al, 2021;Vimala Devi et al, 2021). Talcum powder was used as the carrier substrate, glycerol as the dispersing agent, carboxymethyl cellulose (CMC) as the wetting agent, and a combination of white rice and wheat bran (1:2 w/w) as the bulking agent.…”

Section: Development and Formulation Of Biofungicidesmentioning

confidence: 99%

“…To develop the WDG, the dispersion agent (10 g/kg), wetting agent (50 g/kg), and bulking agent (440 g/kg) were combined with adsorbed substrate (500 g/kg) (Jangir et al, 2021;Vimala Devi et al, 2021). After adding autoclaved water (15 to 20%), the product was mixed well, and the paste fed manually through an extruder machine using a 0.3-mm mesh screen.…”

Section: Development and Formulation Of Biofungicidesmentioning

confidence: 99%

Biological management of coffee wilt disease (Fusarium xylarioides) using antagonistic Trichoderma isolates

et al. 2023

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Coffee wilt disease (CWD) is a serious threat to the food security of small-scale farmers in Ethiopia, causing significant reductions in coffee yield. Currently, there are no effective control measures available against the causative agent of CWD, Fusarium xylarioides. The main objective of this study was therefore to develop, formulate, and evaluate a range of biofungicides against F. xylarioides, derived from Trichoderma species and tested under in vitro, greenhouse, and field conditions. In total, 175 Trichoderma isolates were screened as microbial biocontrol agents against F. xylarioides. The efficacy of two biofungicide formulations, wettable powder and water dispensable granules, were tested on the susceptible Geisha coffee variety in three different agro-ecological zones in southwestern Ethiopia over three years. The greenhouse experiments were set up using a complete block design, while in the field a randomized complete block design was used, with twice yearly applications of biofungicide. The test pathogen spore suspension was applied to the coffee seedlings by soil drenching, and the subsequent incidence and severity of CWD evaluated annually. The mycelial growth inhibition profiles of the Trichoderma isolates against F. xylarioides ranged from 44.5% to 84.8%. In vitro experiments revealed that T. asperelloides AU71, T. asperellum AU131 and T. longibrachiatum AU158 reduced the mycelial growth of F. xylarioides by over 80%. The greenhouse study indicated that wettable powder (WP) of T. asperellum AU131 had the highest biocontrol efficacy (84.3%), followed by T. longibrachiatum AU158 (77.9%) and T. asperelloides AU71 (71.2%); they also had a significant positive impact on plant growth. The pathogen-treated control plants had a disease severity index of 100% across all the field experiments, and of 76.7% in the greenhouse experiments. In comparison to untreated controls, the annual and cumulative disease incidence over the three years of the study period varied from 46.2 to 90%, 51.6 to 84.5%, and 58.2 to 91%, at the Teppi, Gera and Jimma field experimental locations. Overall, the greenhouse and field experiments and in vitro assays support the biocontrol potential of Trichoderma isolates, and T. asperellum AU131 and T. longibrachiatum AU158 in particular are recommended for the management of CWD under field conditions.

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“…Yasin et al (2020) [12] revealed that the application of NPV effective against populations of S. litura in vitro conditions. According to Vimala Devi et al (2021) [11] that the Bt-127 WDG formulation was effective against early as well as older instar larvae S. litura. Gadhiya (2012) [5] revealed that the SlNPV @ 250 LE/ha, azadiractin @ 0.4 per cent, neem oil @ 0.3 per cent and neem seed kernel extract @ 5 per cent gave effective control of S. litura on groundnut crop.…”

Section: Over All Pooledmentioning

confidence: 99%

Effectiveness of biopesticides against Spodoptera litura infesting groundnut under field condition

Dodiya¹,

Barad²

2022

Pharma Innovation

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Among the eight tested biopesticides, SlNPV 250 LE, B. bassiana 1% WP and aqueous bidi tobacco dust extract 2% found most effective and recorded minimum larval population of S. litura as well as per cent damaged groundnut plant. While, treatments of neem oil 1%, Btk 1% WP and M. anisopliae 1% WP were found moderately effective in reducing larval population and per cent damaged plant. Whereas, the treatments of azadirachtin 10000 ppm and N. rileyi 1% WP. Efficacy of biopesticides as reduction in S. litura population was SlNPV 250 LE > B. bassiana 1% WP > aqueous bidi tobacco dust extract 2% > neem oil 1% > Btk 1% WP > M. anisopliae 1% WP > azadirachtin 10000 ppm > N. rileyi 1% WP.

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Development of a water dispersible granule (WDG) formulation of Bacillus thuringiensis for the management of Spodoptera litura (Fab.)

Cited by 8 publications

References 19 publications

Relative activity of 15 bacterial strains against the larvae of Helicoverpa armigera, Spodoptera exigua, and Spodoptera litura (Lepidoptera: Noctuidae)

Relative activity of 15 bacterial strains against the larvae of Helicoverpa armigera, Spodoptera exigua, and Spodoptera litura (Lepidoptera: Noctuidae)

Biological management of coffee wilt disease (Fusarium xylarioides) using antagonistic Trichoderma isolates

Effectiveness of biopesticides against Spodoptera litura infesting groundnut under field condition

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