Development of a visual loop-mediated isothermal amplification method for rapid detection of the bacterial pathogen Pseudomonas putida of the large yellow croaker (Pseudosciaena crocea)

Development of Loop‐mediated Isothermal Amplification (LAMP) for the Rapid Detection of Streptococcus agalactiae in Tilapia, Oreochromis niloticus

“…When comparing the sensitivity of LAMP and PCR through a common method (Notomi et al ; Cai et al ), the LAMP assay was able to detect 20 cells per reaction compared with 200 cells for PCR. The detection limit ratio of LAMP and PCR was similar to other studies using LAMP for bacterial detection of other species (Cai et al ; Mao et al ; Zhang et al ). In the real‐time LAMP assay, it was observed that the time to amplify was shorter for samples with high bacteria densities than with low bacteria densities.…”

“…Because SYBR Green can inhibit the LAMP reaction, it cannot be added before the start of the reaction (Tao et al ). There is a study using microcrystalline wax capsule as a dye container to develop a closed‐tube LAMP system (Mao et al ). However, production of the wax capsule is difficult and requires sophisticated techniques.…”

Challenging loop—mediated isothermal amplification (LAMP) technique for molecular detection of Toxoplasma gondii

“…The major potential pitfall of the LAMP is extremely easy to contaminate a laboratory by simply opening a reaction tube. Measurement of the turbidity [17], changing color using the Calcein or Hydroxy naphthol blue [46,47], entrapping the fluorescent compound into the wax beads that melt at 80°曟 and released it in the last step of the reaction [48] are several methods which have been developed to obtain results without even opening the tube. To avoid the postamplification contamination, the strict spatial separation of reagent preparations and test procedures is also required.…”

Phenotypic and Genotypic Characterization of Some Pseudomonas sp. Associated with Burkholderia cepacia Isolated from Various Infected Fishes

“…Previous studies reported that P. putida is considered one of the serious bacterial pathogens that have endangered the aquaculture of various fishes such as rainbow trout, European eel, oyster toadfish and large yellow croaker [5,[20][21][22]; thus, causing high mortality and resulting in severe economic loss [23].…”