Development of a Visible Spectrophotometric Method for the Analysis of Ganciclovir in Bulk Sample and Dosage Form

Thomas, Olusegun E.; Adegoke, Olajire A.

doi:10.4314/tjpr.v14i6.23

Cited by 9 publications

(6 citation statements)

References 12 publications

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“…The solutions of DMAB between 0.5% and 3% were supersaturated and the reaction mixture had precipitates which scattered light and resulted in high absorbance values. The result obtained here is in consonance with previous application of DMAB as a coupling component for the spectrophotometric determination of diazotizable drugs [24][25][26][27].…”

Section: Effect Of Dmab Concentrationsupporting

confidence: 91%

“…This also suffers the demerit of using high concentration levels of 10-50 μg/mL. In recent years, we have demonstrated the accurate and simple determination of diazotizable drug molecules using p-dimethylaminobenzaldehyde (DMAB) as the coupling component [24][25][26][27]. The present study seeks to extend the advantages of speed, simplicity, accuracy and excellent calibration range to the determination of GBP in bulk and dosage form and using readily available laboratory reagents.…”

Section: Introductionmentioning

confidence: 99%

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New spectrophotometric method for the determination of gabapentin in bulk and dosage forms using p-dimethylaminobenzaldehyde

Adegoke

Adegbolagun

Aiyenale

et al. 2018

Journal of Taibah University for Science

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A new simple, accurate and economic spectrophotometric method based on azo dye derivatization for the determination of gabapentin (GBP) was developed. Critical factors were optimized. The method was validated and assay of dosage forms was done. Spot tests and TLC confirmed the formation of azo adduct. A 0.3 M NaNO 2 solution using 2 M HCl was used for diazotization. The optimal temperature and time were 30°C and 10 min. Azo adducts were determined at 430 nm. Methanol was found to be the best solvent. Gabapentin coupled at a ratio of 1:1 with DMAB. The assays of GBP were linear over the range 1-6 µg/mL (r = 0.9973) and LOD of 0.8322 µg/mL. The methods were accurate (error < 2%) and precise (RSD < 0.5%). The methods were successfully applied to the assay of GBP in dosage forms and compared favorably with reference method (p > .05). The successful diazotization of gabapentin and the azo adduct formation with DMAB is reported for the first time.

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Section: Effect Of Dmab Concentrationsupporting

confidence: 91%

Section: Introductionmentioning

confidence: 99%

New spectrophotometric method for the determination of gabapentin in bulk and dosage forms using p-dimethylaminobenzaldehyde

Adegoke

Adegbolagun

Aiyenale

et al. 2018

Journal of Taibah University for Science

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“…Due to the special molecular structure, the charge-transfer complex or electron-donor-acceptor complex of GCV was investigated for determination of trace amount of GCV by spectroscopic methods. UV-Vis spectrophotometry [9], Raman spectroscopy [10] and capillary electrophoretic method have been reported to quantify the trace amounts of GCV in biological samples [11].…”

Section: Introductionmentioning

confidence: 99%

A Sensitive Electrochemical Sensor for Voltammetric Determination of Ganciclovir Based on Au‐ZnS Nanocomposite

Yari

Shams

2018

Electroanalysis

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An electrode modified with ZnS and gold nanoparticles (Au‐ZnS NPs) is introduced for highly sensitive voltammetric determination of ganciclovir (GCV). Surface structure and topography of the modified electrode was studied by SEM, EDX and XRD techniques. Electrochemical oxidation of GCV was investigated by cyclic (CV) and square wave voltammetry (SWV) in Briton‐Robinson buffer solution (pH 1.5). The results showed that electrochemical oxidation of GCV at the Au‐ZnS modified glassy carbon electrode (GCE) is irreversible and exhibited diffusion controlled electrode process over the pH range from 1.0 to 6.0. The oxidation potential peak and pH relationship showed that electrons and protons were transferred simultaneously over the electrochemical oxidation process. Using the proposed sensor, the linear calibration curves were obtained in the ranges of 0.04–1.50 μM and 1.5–70.0 μM with detection limit of 0.01 μM GCV by SWV technique. The modified electrode was successfully applied as a sensitive, reproducible and repeatable sensor for determination of the trace amount of GCV in human serum, urine and cymevene vials. Reasonable results were obtained from comparing the measurements of the real samples by the new sensor to high performance liquid chromatography (HPLC) as a standard method.

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“…In general, the analysis of pharmaceutical products derived from guanine uses UV-Vis spectrophotometry [7][8][9][10][11][12] and chromatography e. g., HPLC coupled to different types of detectors [13][14][15]. However, these methods are cumbersome and present several disadvantages: the need to carry out derivatization reactions, long waiting times, instability of the colour of the generated compound and the use of non-aqueous media, among others.…”

Section: Introductionmentioning

confidence: 99%

Electrochemical Behaviour Study and Determination of Guanine, 6‐Thioguanine, Acyclovir and Gancyclovir on Fluorine‐doped SnO₂Electrode. Application in Pharmaceutical Preparations

et al. 2017

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An electroanalytical method using square wave voltammetry (SWV) is proposed for the quantification of Guanine (G), 6‐Thioguanine (TG), Acyclovir (Acy) and Ganciclovir (Gan) in different types of pharmacological samples. In addition, for the first time, a study of the electrochemical behavior (using cyclic voltammetry, CV) of guanine and its pharmacological derivatives on fluoride doped oxide (FTO) electrodes was carried out. The study of CV potential scanning demonstrated the charge transfer process was diffusion controlled and the diffusion coefficient was determined for all the studied products. Besides, electrochemical parameters, such as transfer coefficient, heterogeneous charge transfer constant and the number of electrons transferred (e) were determined namely, 4e for TG and 2e for G, Acy and Gan. SWV studies at pH 4 for G and 2 for TG, Acy and Gan showed linear ranges between 4.0×10−6 and 40.0×10−6 mol L−1, with detection limits of 0.91×10−6 for G, and 1.50×10−6 for TG, 1.25×10−6 for Acy and 0.45×10−6 mol L−1 for Gan. The method presents sensitivity and stability appropriate to be employed in quality control and routine quantification of drugs in pharmaceutical formulations.

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Development of a Visible Spectrophotometric Method for the Analysis of Ganciclovir in Bulk Sample and Dosage Form

Cited by 9 publications

References 12 publications

New spectrophotometric method for the determination of gabapentin in bulk and dosage forms using p-dimethylaminobenzaldehyde

New spectrophotometric method for the determination of gabapentin in bulk and dosage forms using p-dimethylaminobenzaldehyde

A Sensitive Electrochemical Sensor for Voltammetric Determination of Ganciclovir Based on Au‐ZnS Nanocomposite

Electrochemical Behaviour Study and Determination of Guanine, 6‐Thioguanine, Acyclovir and Gancyclovir on Fluorine‐doped SnO₂Electrode. Application in Pharmaceutical Preparations

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Development of a Visible Spectrophotometric Method for the Analysis of Ganciclovir in Bulk Sample and Dosage Form

Cited by 9 publications

References 12 publications

New spectrophotometric method for the determination of gabapentin in bulk and dosage forms using p-dimethylaminobenzaldehyde

New spectrophotometric method for the determination of gabapentin in bulk and dosage forms using p-dimethylaminobenzaldehyde

A Sensitive Electrochemical Sensor for Voltammetric Determination of Ganciclovir Based on Au‐ZnS Nanocomposite

Electrochemical Behaviour Study and Determination of Guanine, 6‐Thioguanine, Acyclovir and Gancyclovir on Fluorine‐doped SnO2Electrode. Application in Pharmaceutical Preparations

Contact Info

Product

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Electrochemical Behaviour Study and Determination of Guanine, 6‐Thioguanine, Acyclovir and Gancyclovir on Fluorine‐doped SnO₂Electrode. Application in Pharmaceutical Preparations