Abstract:The aim of this work was to develop and validate RP-HPLC method for quantification of 4 major polyphenolic compounds of mulberry leaf infusion. The mulberry leaf samples were extracted by simulation of tea infusion beverage preparation. HPLC-DAD analysis combined with column C-18 150 mm x 4.6 mm, 2.7 μm was used to determine bioactive polyphenols such as chlorogenic acid, caffeic acid, rutin, and quercetin. The optimal conditions involved the flow rate of mobile phase at 0.3 ml/min with gradient elution of 0.1… Show more
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