Development of a validated liquid chromatographic method for the quality control of Prunellae Spica: Determination of triterpenic acids

Lee, Mi Kyoung; Ahn, Young Min; Lee, Kang Ro; Jung, Jee H.; Jung, Ok‐Sang; Hong, Jongki

doi:10.1016/j.aca.2008.12.038

Cited by 60 publications

(29 citation statements)

References 26 publications

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“…For the separation and determination of oleanolic acid and ursolic acid, thin layer chromatography (TLC) [10], highperformance TLC [11], gas chromatography (GC) [12][13][14][15][16], capillary electrophoresis (CE) [17][18][19], and high-performance liquid chromatography (HPLC) have been reported [20,21]. However, TLC lacks quantitative precision and is mainly used for their identification.…”

Section: Introductionmentioning

confidence: 99%

Determination of oleanolic and ursolic acid in Chinese herbs using HPLC and γ‐CD as mobile phase modifier

Wang¹,

Liu²,

Bing-hua³

et al. 2011

J of Separation Science

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To separate and determine oleanolic acid and ursolic acid, a rapid and accurate HPLC using γ-CD as the mobile phase additive was developed. The effect of CD nature and concentration, and the acidity of the mobile phase on the chromatographic behavior of two bioactive triterpenes were systematically studied. Two bioactive triterpenes were completely separated (R = 3.11) on a Kromasil(®) C(18) column (150×4.6 mm id, 5 μm) with the mobile phase consisting of acetonitrile/0.1% phosphoric acid with 2 mM γ-CD as the mobile phase modifier (60:40, v/v). The flow rate was set at 1.0 mL/min and the eluent was detected at 210 nm for two bioactive triterpenes. The linearity of the method was excellent (r=0.9999) over the studied range of 6-300 μg/mL for oleanolic acid, and 12-600 μg/mL for ursolic acid. The LOD and LOQ were 1.5 and 5.0, 1.0 and 3.0 μg/mL for oleanolic acid and ursolic acid, respectively. The optimized method was successfully applied to separate and determine two bioactive triterpenes in five Chinese herbs. It is concluded that this method could be used for rapid and accurate qualitative and quantitative analysis of the two bioactive triterpenes in Chinese herbs.

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Section: Introductionmentioning

confidence: 99%

Determination of oleanolic and ursolic acid in Chinese herbs using HPLC and γ‐CD as mobile phase modifier

Wang¹,

Liu²,

Bing-hua³

et al. 2011

J of Separation Science

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show abstract

“…[171819] Separation conditions were usually achieved with a reversed phase column filled with C-18 particles between 3 and 5 μm and sample elution with acetonitrile or methanol alongside buffered acetic or phosphoric acid media. The triterpene acids are weak chromophores that are usually detected between 205 and 220 nm.…”

Section: Resultsmentioning

confidence: 99%

Efficiency and selectivity of triterpene acid extraction from decoctions and tinctures prepared from apple peels

Siani¹,

Nakamura²,

Santos³

et al. 2014

Phcog Mag

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Background:This study assessed the extraction efficiency of ursolic (UA) and oleanolic acids (OA), as well as the total phenols in aqueous and hydroethanolic extracts of dry apple peels at room temperature.Materials and Methods:After running preliminary assays on decoctions and tinctures (ethanol: water 7:3 v/v), the extracts from dried apple (cv. Fuji) peels were obtained by static maceration over varied intervals (2 to 180 days). The UA and OA content in the extracts was quantified by High Performance Liquid Chromatography with Diode Array Detection (HPLC-DAD) with a reversed phase column and isocratic elution (CH3CN/H2O/H3PO4) against calibration curves (R2 > 0.9995). The total phenol content in the extracts was evaluated spectrophotometrically at 760 nm using the Folin-Ciocalteau method referencing gallic acid.Results:UA and OA in the hydroethanolic extracts ranged from 3.63-6.12 mg/g and 2.12-3.30 mg/g, corresponding to 1.72-3.07 and 1.00-1.66 mg/g in the raw material, respectively. Higher values of triterpene acid content corresponded to maceration periods of 10 or 30 days. The residual phenol and polyphenol content ranged from 6.97 to 11.6 mg/g. The UA and OA yields, as well as the total phenol content, versus the maceration time were plotted in Control Charts within confidence intervals (95%) and were unaffected during the assayed period.Conclusion:Apple peel tinctures from 10% solids obtained at room temperature exhibited the highest content of triterpene acids when employing a maceration period of 10 to 30 days. Extracts prepared using this procedure contained an average of 7.33 mg/g of total triterpene acids and 10.6 mg/g phenolic compounds. These results establish supporting data for apple peel tinctures and their derived phytopharmaceuticals that are standardized on the ursolic-oleanolic acid content.

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“…The use of marker compounds might not always be suitable due to the lack of unique chemical compounds and ignores the synergic effects between the constituents of complex biological samples [12,13]. To counter the uprising problems, the WHO has introduced fingerprints analysis as a methodology for the assessment of natural products [1].…”

Section: Introductionmentioning

confidence: 99%

Multivariate data analysis to evaluate the fingerprint peaks responsible for the cytotoxic activity of Mallotus species

Tistaert

Châtaigné

Dejaegher

et al. 2012

Journal of Chromatography B

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Development of a validated liquid chromatographic method for the quality control of Prunellae Spica: Determination of triterpenic acids

Cited by 60 publications

References 26 publications

Determination of oleanolic and ursolic acid in Chinese herbs using HPLC and γ‐CD as mobile phase modifier

Determination of oleanolic and ursolic acid in Chinese herbs using HPLC and γ‐CD as mobile phase modifier

Efficiency and selectivity of triterpene acid extraction from decoctions and tinctures prepared from apple peels

Multivariate data analysis to evaluate the fingerprint peaks responsible for the cytotoxic activity of Mallotus species

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