Development of a Vaginal Immobilization Device: A Treatment-planning Study of Carbon-ion Radiotherapy and Intensity-modulated Radiation Therapy for Uterine Cervical Cancer

Kubota, Yoshiki; Ohno, Tatsuya; Kawashima, Motohiro; Murata, Kazutoshi; Okonogi, Noriyuki; Noda, Shin‐ei; Tsuda, Kazuhisa; Sakai, Makoto; Tashiro, Mitsuru; Nakano, Takashi

doi:10.21873/anticanres.13300

Cited by 5 publications

(5 citation statements)

References 15 publications

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“…Surgery and chemotherapy combined with radiotherapy is currently the main treatment for cervical cancer. In recent years, with the advancement and development of radiotherapy technology, its role in the clinic has become increasingly prominent . Unfortunately, the therapeutic effect of radiotherapy has not met our expectations yet, and the reduced sensitivity of cancer cells to radiotherapy is an important cause of recurrence and metastasis in some patients .…”

Section: Discussionmentioning

confidence: 99%

miR‐122‐5p modulates the radiosensitivity of cervical cancer cells by regulating cell division cycle 25A (CDC25A)

Ding

Gao

et al. 2019

FEBS Open Bio

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Cervical cancer is one of the most common gynecological malignancies globally, Unfortunately, radiotherapy and chemotherapy are not effective at treating some cases of this disease, and the 5‐year survival rate is only 40–50%. Cell division cycle 25A (CDC25A) has been shown to induce radioresistance in a variety of tumor cells, but the role of CDC25A in the radioresistance of cervical cancer has not been fully elucidated. Here, we report that CDC25A is highly expressed and miR‐122‐5p lowly expressed in cervical cancer tissues and cells. The TargetScan database was used to predict CDC25A as a target of miR‐122‐5p, and the interactions between miR‐122‐5p and CDC25A were further confirmed by western blot, real‐time PCR and dual‐luciferase reporter assay. Under X‐ray irradiation, up‐regulation of CDC25A can promote the radiation resistance of cervical cancer cells, whereas overexpression of miR‐122‐5p or knockdown of CDC25A inhibits the survival and induces apoptosis of cervical cancer colonies. In conclusion, our data suggest that miR‐122‐5p enhances the radiosensitivity of cervical cancer cells by targeting CDC25A.

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Section: Discussionmentioning

confidence: 99%

miR‐122‐5p modulates the radiosensitivity of cervical cancer cells by regulating cell division cycle 25A (CDC25A)

Ding

Gao

et al. 2019

FEBS Open Bio

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“…With the advancement and development of radiotherapy technology, radiotherapy has become one of the effective methods for the treatment of CC, and its role in the clinic is increasingly significant . However, the reduced sensitivity of cancer cells to radiotherapy is an important cause of recurrence and metastasis in some patients .…”

Section: Discussionmentioning

confidence: 99%

miR‐16‐5p modulates the radiosensitivity of cervical cancer cells via regulating coactivator‐associated arginine methyltransferase 1

Zhang

Wang

et al. 2019

Pathology International

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This study was to investigate the expression of coactivator‐associated arginine methyltransferase 1 (CARM1) and miR‐16‐5p in cervical cancer (CC), and explore their roles in radioresistance. Western blot and immunohistochemistry were used to detect the expression of CARM1 in tissues and cells. Reverse transcription‐polymerase chain reaction (RT‐PCR) was used to detect the expression of miR‐16‐5p. CC cells received different doses of X‐ray exposure, and then cell counting kit‐8 method and colony formation assay were used to detect cell proliferation. Apoptosis was detected by flow cytometry. Then we used Targetscan database to predict that CARM1 is a potential target of miR‐16‐5p, and further verified the targeting relationship between them by western blot, RT‐PCR and dual luciferase reporter experiments. We demonstrated that CARM1 were highly expressed in CC tissues and radio‐resistant CC cells, while miR‐16‐5p expression was low. Under irradiation, up‐regulation of CARM1 can induce radiotherapy resistance of CC cells, while overexpression of miR‐16‐5p or CARM1 knockdown could inhibit the survival of CC cell and induced apoptosis. CARM1 was verified as a target for miR‐16‐5p. Besides, up‐regulation of CARM1 reversed the increase in radiosensitivity induced by miR‐16‐5p. Collectively, we concluded that miR‐16‐5p promoted the radiosensitivity of CC cells by targeting CARM1.

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“…To minimize internal motion, 100–150 mL of normal saline was injected into the bladder. During the CIRT for PTV2 or PTV3, vaginal packing (i.e., cotton pads soaked in contrast medium) or vaginal devices [ 36 ] were used to allow visualization of the surface of the cervix by X-ray.…”

Section: Methodsmentioning

confidence: 99%

Multi-Institutional Retrospective Analysis of Carbon-Ion Radiotherapy for Patients with Locally Advanced Adenocarcinoma of the Uterine Cervix

Okonogi

Ando

Murata

et al. 2021

Cancers

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The clinical significance of carbon-ion radiotherapy (CIRT) for adenocarcinoma (AC) of the uterine cervix has been assessed in several single-institutional studies. To validate the significance, we conducted a multi-institutional survey of CIRT for locally advanced AC (LAAC) of the uterine cervix. We retrospectively analyzed the clinical outcomes of patients with stage IIB–IVA LAAC of the uterine cervix who underwent chemo-CIRT or CIRT alone between April 2010 and April 2016. Patients received 74.4 Gy (relative biological effectiveness [RBE]) in 20 fractions of CIRT or 55.2 Gy (RBE) in 16 fractions of CIRT plus three sessions of brachytherapy. Patients aged ≤ 70 years with adequate bone marrow and organ function were administered cisplatin weekly (40 mg/m2 per week for up to 5 weeks). Fifty-five patients were enrolled in this study. The median follow-up period was 67.5 months. The 5-year overall survival (OS) and local control (LC) rates were 68.6% and 65.2%, respectively. Multivariate analysis showed that the initial tumor response within 6 months was significantly associated with LC and OS. The present study represents promising outcomes of CIRT or chemo-CIRT for LAAC of the uterine cervix, especially in the cases showing initial rapid regression of the tumor.

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Development of a Vaginal Immobilization Device: A Treatment-planning Study of Carbon-ion Radiotherapy and Intensity-modulated Radiation Therapy for Uterine Cervical Cancer

Cited by 5 publications

References 15 publications

miR‐122‐5p modulates the radiosensitivity of cervical cancer cells by regulating cell division cycle 25A (CDC25A)

miR‐122‐5p modulates the radiosensitivity of cervical cancer cells by regulating cell division cycle 25A (CDC25A)

miR‐16‐5p modulates the radiosensitivity of cervical cancer cells via regulating coactivator‐associated arginine methyltransferase 1

Multi-Institutional Retrospective Analysis of Carbon-Ion Radiotherapy for Patients with Locally Advanced Adenocarcinoma of the Uterine Cervix

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