Development of a Timepix based detector for the NanoXCT project

Nachtrab, F.; Hofmann, Thomas; Speier, C.; Lucic, J.; Firsching, Markus; Uhlmann, Norman; Takman, Per; Heinzl, Christoph; Holmberg, Anders; Krumm, M.; Sauerwein, C.

doi:10.1088/1748-0221/10/11/c11009

Cited by 11 publications

(5 citation statements)

References 4 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Unfortunately, a smaller source-to-sample distance could not be tested due to the shape of the resolution pattern housing. Therefore, we were unable to overcome the limitations posed by Fresnel diffraction effects and resolve structures below 200 nm, even though our nanofocus source can create X-ray spots that allow features smaller than this to be visualized in projection images (28).…”

Section: Discussionmentioning

confidence: 99%

Myoanatomy of the velvet worm leg revealed by laboratory-based nanofocus X-ray source tomography

Müller

Oliveira

Allner

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

X-ray computed tomography (CT) is a powerful noninvasive technique for investigating the inner structure of objects and organisms. However, the resolution of laboratory CT systems is typically limited to the micrometer range. In this paper, we present a table-top nanoCT system in conjunction with standard processing tools that is able to routinely reach resolutions down to 100 nm without using X-ray optics. We demonstrate its potential for biological investigations by imaging a walking appendage of , a representative of Onychophora-an invertebrate group pivotal for understanding animal evolution. Comparative analyses proved that the nanoCT can depict the external morphology of the limb with an image quality similar to scanning electron microscopy, while simultaneously visualizing internal muscular structures at higher resolutions than confocal laser scanning microscopy. The obtained nanoCT data revealed hitherto unknown aspects of the onychophoran limb musculature, enabling the 3D reconstruction of individual muscle fibers, which was previously impossible using any laboratory-based imaging technique.

show abstract

Section: Discussionmentioning

confidence: 99%

Myoanatomy of the velvet worm leg revealed by laboratory-based nanofocus X-ray source tomography

Müller

Oliveira

Allner

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

show abstract

“…The in-house-constructed table-top NanoCT setup does not use any X-ray or visible light optics and is based on the principle of a shadow microscope with geometric magnification 10 . The two main components are the nanofocus X-ray source (Nanotube prototype; Excillum) 46 and a single-photon counting detector with a FOV of 1475 pixel × 195 pixel (PILATUS 300K-W 20 Hz, Dectris) 47,48 . The device can reach resolutions in the reconstructed tomography data down to 100 nm 10 .…”

Section: Methodsmentioning

confidence: 99%

Nanoscopic X-ray tomography for correlative microscopy of a small meiofaunal sea-cucumber

Ferstl

Schwaha

Ruthensteiner

et al. 2020

Sci Rep

View full text Add to dashboard Cite

In the field of correlative microscopy, light and electron microscopy form a powerful combination for morphological analyses in zoology. Due to sample thickness limitations, these imaging techniques often require sectioning to investigate small animals and thereby suffer from various artefacts. A recently introduced nanoscopic X-ray computed tomography (NanoCT) setup has been used to image several biological objects, none that were, however, embedded into resin, which is prerequisite for a multitude of correlative applications. In this study, we assess the value of this NanoCT for correlative microscopy. For this purpose, we imaged a resin-embedded, meiofaunal sea cucumber with an approximate length of 1 mm, where microCT would yield only little information about the internal anatomy. The resulting NanoCT data exhibits isotropic 3D resolution, offers deeper insights into the 3D microstructure, and thereby allows for a complete morphological characterization. For comparative purposes, the specimen was sectioned subsequently to evaluate the NanoCT data versus serial sectioning light microscopy (ss-LM). To correct for mechanical instabilities and drift artefacts, we applied an alternative alignment procedure for CT reconstruction. We thereby achieve a level of detail on the subcellular scale comparable to ss-LM images in the sectioning plane.The relevant structures in biological specimens span over a wide range of length scales 1-3 . To obtain a holistic understanding of such structures, correlative microscopy combines multiple imaging techniques to investigate a single sample. It thereby exploits the specific strengths of the different imaging modalities, particularly with respect to sample sizes and resolution, to answer modern life science questions 1,2,4 .Among microscopic techniques correlative light and electron microscopy (CLEM) has been found to be particularly powerful. Both imaging fields are highly complementary 5 . CLEM is a collective term for all different combinations of light and electron microscopy, ranging from fluorescence super-resolution microscopy to conventional bright field light microscopy and from focused-ion-beam scanning electron microscopy (FIB-SEM) over serial-block-face SEM (SBF-SEM) to transmission electron microscopy (TEM) 1,4-6 . Since light and electron microscopy techniques conventionally feature limited penetration depths through matter, they typically rely on sectioning or complete destruction of the sample to create 3D information of entire small biological specimens 1,7,8 . This often prevents isotropic resolution, and the resulting data are affected by deficiencies such as sectioning and alignment artefacts 1,4,5 .X-ray computed tomography (CT) is a widely used tool for non-destructive 3D imaging with isotropic resolution 3,9,10 . Recently, high-resolution X-ray CT (terminology used in this study: microCT resolutions around 1 µm, NanoCT resolutions around 500 nm and smaller) has been integrated in a growing number of correlative studies 1,7,[11][12][13] .Combining different i...

show abstract

“…It is based on the principle of a shadow microscope and, thereby, does not rely on X-ray or visible light optics for magnification. By combining a nanofocus X-ray source (prototype Nanotube; Excillum) [33] with a single-photon counting detector (PILATUS 300K-W 20 Hz, Dectris) [34] it reaches resolutions down to 100 nm [21]. For the presented data,1,599 projections, evenly distributed over 360°, were taken at a peak voltage of 60 kV.…”

Section: F X-ray Nanoct: Acquisitionmentioning

confidence: 99%

Multi-Scale Investigation of Human Renal Tissue in Three Dimensions

Busse

Ferstl

Kimm

et al. 2022

IEEE Trans. Med. Imaging

View full text Add to dashboard Cite

Histopathology as a diagnostic mainstay for tissue evaluation is strictly a 2D technology. Combining and supplementing this technology with 3D imaging has been proposed as one future avenue towards refining comprehensive tissue analysis. To this end, we have developed a laboratory-based X-ray method allowing for the investigation of tissue samples in This paragraph of the first footnote will contain the date on which you submitted your paper for review. We acknowledge financial support through the European's Union Horizon 2020 Research and Innovation Program under the Marie Skłodowska-Curie Grant Agreement H2020-MSCA-IF-2015-703745-CONSALT. M. Busse and S. Ferstl contributed equally to this work. W. Weichert and F. Pfeiffer also contributed equally to this work. M. Busse and M. Dierolf are with the Chair of Biomedical Physics,

show abstract

Development of a Timepix based detector for the NanoXCT project

Cited by 11 publications

References 4 publications

Myoanatomy of the velvet worm leg revealed by laboratory-based nanofocus X-ray source tomography

Myoanatomy of the velvet worm leg revealed by laboratory-based nanofocus X-ray source tomography

Nanoscopic X-ray tomography for correlative microscopy of a small meiofaunal sea-cucumber

Multi-Scale Investigation of Human Renal Tissue in Three Dimensions

Contact Info

Product

Resources

About