Development of a surface plasmon resonance-based immunosensor for the rapid detection of cardiac troponin I

Abstract

The concentration of cardiac troponin I (cTnI) in blood is an important marker for heart muscle cell damage. A surface plasmon resonance (SPR)-based immunosensor was devised for the rapid and specific detection of cTnI. It was constructed by crosslinking a monoclonal antibody P-II-13, which was generated against a loop region (aa 84-94) of cTnI protein as an epitope peptide, onto a chemically modified thin gold film. The performance of the sensor was examined with respect to the SPR signal intensity versus cTnI concentration. The signal intensity was directly correlated with the cTnI concentration in the range of 0-160 μg/l. The sensor signal was saturated when the concentration of cTnI approached 660 μg/l with the SPR intensity of 172 RU. The lower detection limit of the sensor was 68 ng/l cTnI, which was comparable to ELISA-based commercial cTnI detection systems.

Development of the Troponin Detection System Based on the Nanostructure

“…By this modified SPR technique, they could detect a TN at ~1.25 ng/mL. Ro group has reported an SPR-chip TN immunosensor using a TN peptide antigen epitope and an anti-TN monoclonal antibody on an Au thin film-modified slide glass [76]. To fabricate the SPR chip, a TN peptide antigen and an anti-TN monoclonal antibody were prepared by recombinant-DNA techniques and a hybridoma cell-based antibody production method, respectively.…”

Improved performance of near infrared excitation Raman spectroscopy using reflective thin-film gold on glass substrates for cytology samples

“…[36][37][38] Similar thin-lm gold substrates as those used in this study have also previously been in the analysis of surface plasmon resonance for the real-time observation of biomolecular interactions, which involves the direct adhesion of the cells to the surface of the gold. 39,40 We are not aware of any reports that coated gold substrates inhibit cell growth. Although, in recent work osteosarcoma cells were grown on stainless steel substrates for analysis with Raman spectrocopy, 20 thin-lm gold substrates may be preferable in terms of biocompatibility, with the latter having been shown to negatively impact on cell growth in some cases.…”

Functionalized Graphene Quantum Dot Interfaced Electrochemical Detection of Cardiac Troponin I: An Antibody Free Approach

“…cTnI is released directly into the blood and its concentration in healthy people is less than 0.4 ng mL −1 , while concentrations greater than 2.0 ng mL −1 indicate the presence of AMI 7 . Conventional methods used to detect cTnI are surface plasmon resonance (SPR) 8 . chemiluminescence, enzyme-linked immunosorbent assay(ELISA) 9 .…”