“…The assays based on immunology include colloidal gold test strip assay [ 4 ], time-resolved fluorescence immunoassay [ 5 ], fluorescent immunochromatography test strip [ 6 ], amplified luminescent proximity homogeneous assay (AlphaLISA) [ 7 ], triplex bead-based assay for the simultaneous detection of antibodies [ 8 ], Eu (III) chelate microparticle-based lateral flow test strip and blocking enzyme-linked immunosorbent assay [ 2 9 ]. Many nucleic acid amplification methods have been improved or modified for the detection of ASFV, such as multiplex reverse transcription-polymerase chain reaction (RT-PCR) assay [ 10 ], triplex real-time PCR assay [ 11 ], real-time loop-mediated isothermal amplification (LAMP) assay [ 12 13 ], LAMP combined with lateral flow dipstick [ 14 ], semi-quantitative colorimetric LAMP [ 15 ], recombinase polymerase amplification (RPA) combined with lateral flow [ 16 17 18 ], PCR with a lateral flow strip [ 19 ], improved real-time PCR system with probe modification [ 20 ], CRISPR-Cas12a system coupled with PCR or LAMP [ 21 ], recombinase aided amplification combined with CRISPR/Cas12a [ 22 ], RPA-CRISPR-based nucleic acid detection method [ 23 ] and Crispr/cas12a technology combined with immunochromatographic strips [ 24 ]. The microfluidic-circular fluorescent probe-mediated isothermal nucleic acid amplification (CFPA) system has been newly developed for the detection of ASFV [ 25 ], and advanced nanopore sequencing has also been used for the diagnosis of ASFV [ 26 ].…”