Development of a storage method for ascospores of<i>Sclerotinia sclerotiorum</i>

Olivier, Chrystel; Séguin-Swartz, G.

doi:10.1080/07060660609507324

Cited by 1 publication

(1 citation statement)

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“…Few long‐term studies have been published on fungal storage at low temperatures and, of these, mixed success has been reported. Olivier and Séguin‐Swartz reported a near 100% germination rate at 12 months and 50‐60% at 24 months of Sclerotinia sclerotiorium ascospores (sexual spores) stored in 30% glycerol at −80 °C. Elliot demonstrated that the ability to recover Gaeumannomyces graminis from mycelial plugs stored in 40% glycerol at −75 °C is strain‐specific, and unable to be sustained for more than 48 months.…”

Section: Discussionmentioning

confidence: 99%

Yield and cold storage of Trichoderma conidia is influenced by substrate pH and storage temperature

et al. 2017

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In this study we examined the influence of the ambient pH during morphogenesis on conidial yield of Trichoderma sp. "atroviride B" LU132 and T. hamatum LU593 and storage at low temperatures. The ambient pH of the growth media had a dramatic influence on the level of Trichoderma conidiation and this was dependent on the strain and growth media. On malt-extract agar, LU593 yield decreased with increasing pH (3-6), whereas yield increased with increasing pH for LU132. During solid substrate production the reverse was true for LU132 whereby yield decreased with increasing pH. The germination potential of the conidia decreased significantly over time in cold storage and the rate of decline was a factor of the strain, pH during morphogenesis, growth media, and storage temperature.

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Section: Discussionmentioning

confidence: 99%