Development of a Stability-Indicating LC Assay Method for Determination of Chloroquine

Rivas-Granizo, Patricia Elizabeth; Santos, S R C J; Ferraz, Humberto Gomes

doi:10.1365/s10337-009-0985-3

Cited by 8 publications

(4 citation statements)

References 12 publications

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“…Alkalinizing aqueous phase with NaHCO 3 for pH = 8.4 in Formulation 5 proved to be more efficient, inducing an average EE% of about 25%. Previous studies have reported that CQ degradation is accelerated in 0.1 M NaOH media, by a hydrolysis mechanism [50]. The hypothesis of drug precipitation in the aqueous phase, due to the strongest alkaline pH, should also be considered.…”

Section: Discussionmentioning

confidence: 99%

Improving Encapsulation of Hydrophilic Chloroquine Diphosphate into Biodegradable Nanoparticles: A Promising Approach against Herpes Virus Simplex-1 Infection

et al. 2018

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Chloroquine diphosphate (CQ) is a hydrophilic drug with low entrapment efficiency in hydrophobic nanoparticles (NP). Herpes simplex virus type 1 (HSV-1) is an enveloped double-stranded DNA virus worldwide known as a common human pathogen. This study aims to develop chloroquine-loaded poly(lactic acid) (PLA) nanoparticles (CQ-NP) to improve the chloroquine anti- HSV-1 efficacy. CQ-NP were successfully prepared using a modified emulsification-solvent evaporation method. Physicochemical properties of the NP were monitored using dynamic light scattering, atomic force microscopy, drug loading efficiency, and drug release studies. Spherical nanoparticles were produced with modal diameter of <300 nm, zeta potential of −20 mv and encapsulation efficiency of 64.1%. In vitro assays of CQ-NP performed in Vero E6 cells, using the MTT-assay, revealed different cytotoxicity levels. Blank nanoparticles (B-NP) were biocompatible. Finally, the antiviral activity tested by the plaque reduction assay revealed greater efficacy for CQ-NP compared to CQ at concentrations equal to or lower than 20 µg mL−1 (p < 0.001). On the other hand, the B-NP had no antiviral activity. The CQ-NP has shown feasible properties and great potential to improve the antiviral activity of drugs.

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Section: Discussionmentioning

confidence: 99%

Improving Encapsulation of Hydrophilic Chloroquine Diphosphate into Biodegradable Nanoparticles: A Promising Approach against Herpes Virus Simplex-1 Infection

et al. 2018

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“…By far, the most common RP-HPLC approach is to carry out the separation using some type of octadecyl modified packing (i.e., porous silica-based) in combination with either a binary aqueous-acetonitrile − or aqueous-methanol − eluent. Examples of these methods are given in Table .…”

Section: Separation-based Methodologymentioning

confidence: 99%

Pharmaceuticals and Related Drugs

Gilpin

2011

Anal. Chem.

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“…Other analytical methods reported for the assay of CLQ in pharmaceuticals include visible spectrophotometry [18][19][20][21] , spectrofluorimetry 22 , highperformance liquid chromatography [23][24][25] .…”

Section: Fig 3: Chemical Structure Of Chloroquine Phosphatementioning

confidence: 99%

Untitled

2019

IJPSR

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Simple, sensitive, cost-effective and selective methods are designed and developed for the oxidative indirect spectrophotometric determination of drugs and pharmaceuticals, viz., Argatrobane Monohydrate (ARG), Bortezomib (BOR), Chloroquine Phospate (CHP), Granisetron HCl (GRA), Ibandronate Sodium (IBA) based on their reactivity towards nbromo succinamide (NBS in excess). The method is based on the oxidation of drugs by n-bromo succinamide (excess) and estimating the amount of unreacted NBS by Methyl Orange dye at λ max 508 nm. The calibration curves obeyed Beer's law over the concentration range of 10-70 µg mL-1 (ARG), 8-56 µg mL-1 (BOR), 6-42 µg mL-1 (CHP), 4-28 µg mL-1 (GRA) & 5-35 µg mL-1 (IBA). This method has been applied for the determination of drugs in their pure form as well as in tablet formulations. Statistical analysis of the results using Student's t-test for accuracy and F-test for precision revealed no significant difference between the proposed methods and the literature method at the 95% confidence level with respect to accuracy and precision. The method has been validated in terms of guidelines of the International Council of Harmonization. INTRODUCTION: Argatrobane Monohydrate: Argatrobane monohydrate, ARG Fig. 1 is chemically [((2R, 4R)-4-methyl-1,2,3,4-tetrahydro-8-quinolinesulfonyl)-L-arginyl-2-piperidinecarboxylic acid mono hydrate]. It is a direct thrombin inhibitor under clinical development as adjunctive therapy to thrombolytic agents in acute myocardial infarction (AMI). Recent clinical trials have shown argatroban to be especially effective when administered in conjunction with a thrombolytic agent within 6 h of the onset of AMI symptoms.

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Development of a Stability-Indicating LC Assay Method for Determination of Chloroquine

Cited by 8 publications

References 12 publications

Improving Encapsulation of Hydrophilic Chloroquine Diphosphate into Biodegradable Nanoparticles: A Promising Approach against Herpes Virus Simplex-1 Infection

Improving Encapsulation of Hydrophilic Chloroquine Diphosphate into Biodegradable Nanoparticles: A Promising Approach against Herpes Virus Simplex-1 Infection

Pharmaceuticals and Related Drugs

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