Development of a sperm zona pellucida binding assay for bull semen

Fazeli, Alireza; Steenweg, W.; Bevers, M.M.; Loos, F.A.M. de; Broek, J. van den; Colenbrander, B.

doi:10.1136/vr.132.1.14

Cited by 64 publications

(35 citation statements)

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“…Human sperm binding to homologous ZP highly correlates with male fertility and outcomes of IVF (Liu et al 1989, Oehninger et al 1989, Gamzu et al 1994. In similar experiments, binding ability of sperm to the ZP correlated with fertility of bulls (Fazeli et al 1993), boars (Ivanova & Mollova 1993, Braundmeier et al 2004, and stallions (Fazeli et al 1995, Pantke et al 1995, Meyers et al 1996. However, in the horse, use of ZP-binding assays has been limited because of the scarcity of equine oocytes.…”

Section: Introductionmentioning

confidence: 90%

Effects of components of semen extenders on the binding of stallion spermatozoa to bovine or equine zonae pellucidae

Silva

Seidel²,

Squires³

et al. 2012

Reproduction

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The effects of semen extender components on the ability of stallion sperm to bind to the zona pellucida (ZP) and the suitability of using bovine ZP for a ZP-binding assay for stallion sperm were investigated in a series of experiments. In Experiment I, binding of stallion sperm to both bovine and equine ZP was significantly increased when a skim milk-based extender (EZM) was used. In Experiment II, a threefold increase in sperm binding to ZP was observed when sperm were diluted in EZM compared with diluents, which contained no milk (TALP, LAC, and EmCare). In Experiment III, centrifuging the sperm through Percoll did not increase sperm binding to the ZP but did remove any positive effect of EZM on sperm-ZP binding. In Experiment IV, exposure of either sperm or ZP to EZM before co-incubation did not increase sperm binding to ZP. In Experiment V, sperm diluted in TALP containing skim milk, EZM, or INRA96 bound more efficiently to the ZP than sperm diluted in TALP without milk proteins. In Experiment VI, sodium caseinate, native phosphocaseinate, and caseinoglycopeptide increased sperm binding to the ZP. In conclusion, diluents containing milk or milk proteins markedly enhanced the number of sperm bound to both equine and bovine ZP.

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Section: Introductionmentioning

confidence: 90%

Effects of components of semen extenders on the binding of stallion spermatozoa to bovine or equine zonae pellucidae

Silva

Seidel²,

Squires³

et al. 2012

Reproduction

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“…Various functional tests have been applied to sperm including assessment of acrosome reaction induction (Lenz et al, 1988;Parkinson, 1992, 1995;Janskauskas et al, 2000), heparin binding to sperm (Marks and Ax, 1985), sperm-zona binding assays (Fazeli et al, 1993;Zhang et al, 1998), and sperm penetration assays (Ohgoda et al, 1988;Henault and Killian, 1995). Advances in bovine IVF and embryo culture techniques have allowed the relationship between in vivo bull fertility and IVF outcomes to be examined (Hillery et al, 1990;Marquant-Le Guienne et al, 1990;Shamsuddin and Larsson, 1993;Lonergan, 1994;Schneider et al, 1999;Larsson and RodriguezMartinez, 2000).…”

Section: Introductionmentioning

confidence: 99%

Paternal influence on the time of first embryonic cleavage post insemination and the implications for subsequent bovine embryo development in vitro and fertility in vivo

Ward

Rizos

Corridan

et al. 2001

Molecular Reproduction Devel

127

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The objectives of this study were: (1) to evaluate the effect of sire on the time from insemination to first cleavage following insemination in vitro and the relationship of this parameter to field fertility and (2) to establish the relationship between the kinetics of cleavage in vitro and oocyte developmental competence for bulls of known field fertility. Frozen semen from six bulls with 150-day non-return rates ranging from 57±78% was used. In experiment 1, after insemination with semen from one of the six bulls, presumptive zygotes were transferred to IVC in droplets of synthetic oviduct fluid. Droplets were examined at 24, 27, 30, 33, 36, 42, and 48 hr after insemination and the number of cleaved oocytes was recorded. Blastocyst yield was recorded on Days 6-, 7-, and 8-post insemination. In experiment 2, culture droplets were examined at 30, 36, and 48 hr after insemination. At each time point, the number of cleaved embryos was recorded and these embryos were transferred into new droplets and were cultured separately for the duration of the experiment. The proportion of embryos developing to the blastocyst stage was recorded for each of the groups for each bull. The best predictor of field fertility was a model containing 33-hpi-cleavage percentage only (r 0.689, P`0.0001). There was also a significant correlation between blastocyst yield and non-return rate, with Day 7 blastocyst yield having the highest correlation (r 0.356), although this was relatively low in comparison. In experiment 2, irrespective of sire, a significantly higher proportion of those early-cleaving oocytes (before 30 hpi) developed to blastocysts than those cleaving later. In most cases, a higher proportion of blastocysts derived from early-cleaving oocytes hatched from the zona pellucida suggesting that such blastocysts are of superior quality to those derived from late-cleaving oocytes. In conclusion these data confirm our earlier observations that earliest cleaving zygotes are more competent in terms of development to the blastocyst stage than those that cleave later. This phenomenon is independent of the sire used. However, we have demonstrated that the kinetics of early embryonic development as measured by the timing of the first cleavage division post insemination vary between different bulls and that these differences can be used to discriminate between bulls of high and low bull field fertility. Mol. Reprod. Dev. 60: 47±55,

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“…The oocytes were co-incubated with sperm at a final concentration of 10 5 sp/ml. After 4 h of co-incubation (Fazeli et al 1993), the oocytes were washed thrice to remove loosely attached spermatozoa, and subsequently fixed and stained (as described in the previous experiment). Per presumed zygote, the number of spermatozoa bound to the ZP was determined.…”

Section: Effect Of Fn On Sperm-zona Bindingmentioning

confidence: 99%

Expression and putative function of fibronectin and its receptor (integrin α5β1) in male and female gametes during bovine fertilization in vitro

Thys¹,

Nauwynck²,

Maes³

et al. 2009

Reproduction

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Fibronectin (Fn) is a 440 kDa glycoprotein assumed to participate in sperm-egg interaction in human. Recently, it has been demonstrated that Fn -when present during bovine IVF -strongly inhibits sperm penetration. The present study was conducted firstly to evaluate the expression of Fn and its integrin receptor (a 5 b 1 ) on male and female bovine gametes using indirect immunofluorescence and secondly, to determine the function of Fn during bovine IVF. Endogenous Fn was detected underneath the zona pellucida (ZP) and integrin a 5 on the oolemma of cumulus-denuded oocytes. Bovine spermatozoa displayed integrin a 5 at their equatorial segment after acrosome reaction. We established that the main inhibitory effect of exogenously supplemented Fn was located at the sperm-oolemma binding, with a (concurrent) effect on fusion, and this can probably be attributed to the binding of Fn to spermatozoa at the equatorial segment, as shown by means of Alexa Fluor 488-conjugated Fn. Combining these results, the inhibitory effect of exogenously supplemented Fn seemed to be exerted on the male gamete by binding to the exposed integrin a 5 b 1 receptor after acrosome reaction. The presence of endogenous Fn underneath the ZP together with integrin a 5 expression on oolemma and acrosome-reacted (AR) sperm cell surface suggests a 'velcro' interaction between the endogenous Fn ligand and corresponding receptors on both (AR) sperm cell and oolemma, initiating sperm-egg binding.

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Development of a sperm zona pellucida binding assay for bull semen

Cited by 64 publications

References 0 publications

Effects of components of semen extenders on the binding of stallion spermatozoa to bovine or equine zonae pellucidae

Effects of components of semen extenders on the binding of stallion spermatozoa to bovine or equine zonae pellucidae

Paternal influence on the time of first embryonic cleavage post insemination and the implications for subsequent bovine embryo development in vitro and fertility in vivo

Expression and putative function of fibronectin and its receptor (integrin α5β1) in male and female gametes during bovine fertilization in vitro

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