Development of a Species-Specific PCR Assay for Correct Labeling of Eight Squid Species from Loliginidae and Ommastrephidae Families (Mollusca: Cephalopoda)

Wang, Hongtao; Tian, Hui-Li; Hao, Na; Wang, Xinqi

doi:10.1007/s12161-018-1287-x

Cited by 5 publications

(2 citation statements)

References 18 publications

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“…Therefore, full-length DNA barcoding sequences are very di cult to be ampli ed from deeply processed products. Species-speci c PCR refers to PCR ampli cation using speci c primers for distinguishing related samples (Wang et al 2018). This method is based on the fact that mismatches of speci c primers can lead to failure in ampli cation.…”

Section: Discussionmentioning

confidence: 99%

Development of a reliable DNA method for quality control of Glehnia littoralis and its industrial extracts

Lin

Wang

et al. 2022

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As a medicinal and edible plant, Glehnia littoralis has been widely recognized for its comprehensive nutritional properties and pharmaceutical effects, but it has also been the target of fraudulent practices. To overcome the difficulties in unanimous polymorphism exploitation of the adulterants and DNA degradation of industrial extracts, ribosomal ITS and chloroplast trnL-F gene sequences were targeted for molecular analysis. Based on the developed single nucleotide polymorphism (SNP) markers, two species-specific PCR systems were respectively established and proved to be effective for molecular discrimination of G. littoralis from its adulterants. The SNP marker exploited in trnL-F region was able to detect 0.1% of intentional adulteration of genomic DNA with a detection limit of 0.01 ng, and it is effective for botanical origin authentication of G. littoralis industrial extracts either in single or mixed plant samples. Compared with the other molecular protocols so far used, the established DNA method enabled the accurate authentication and standardization of routine work for quality control of G. littoralis and its industrial products.

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Section: Discussionmentioning

confidence: 99%

Development of a reliable DNA method for quality control of Glehnia littoralis and its industrial extracts

Lin

Wang

et al. 2022

Preprint

Self Cite

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“…Species-speci c PCR refers to PCR ampli cation using speci c primers for distinguishing related samples (Wang et al 2018). This method is based on the fact that mismatches of speci c primers can lead to failure in ampli cation and can detect trace differences in base pair sequence.…”

Section: Discussionmentioning

confidence: 99%

A Sensitive and Robust DNA Method for Authenticity Determination of Glehnia littoralis and Its Food Products

Lin

Wang

et al. 2022

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Glehnia littoralis has been widely recognized for its comprehensive nutritional properties and pharmaceutical effects. However, the adulteration practices with cheaper or very similar species in its food products lead to unfair market competition and severe health risks to the consumers. In this study, two species-specific PCR systems were constructed for molecular discrimination of G. littoralis from its adulterants based on the single nucleotide (SNP) markers exploited from ribosomal ITS and chloroplast trnL-F regions, respectively. The species-specific PCR assay utilizing trnL-F region was able to detect 0.1% of intentional adulteration of genomic DNA with a detection limit of 0.01 ng. The developed method, mitigating the adverse effects of DNA degradation and false negative results, was proved to be effective for botanical origin authentication of G. littoralis food products in a single reaction. Therefore the present study provides a sensitive and robust DNA method for authenticity determination of G. littoralis and its food products, and the established method can be employed to determine the authenticity and adulteration of other agro-products with high accuracy and reliability.

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From DNA barcoding to nanoparticle-based colorimetric testing: a new frontier in cephalopod authentication

et al. 2020

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Development of a Species-Specific PCR Assay for Correct Labeling of Eight Squid Species from Loliginidae and Ommastrephidae Families (Mollusca: Cephalopoda)

Cited by 5 publications

References 18 publications

Development of a reliable DNA method for quality control of Glehnia littoralis and its industrial extracts

Development of a reliable DNA method for quality control of Glehnia littoralis and its industrial extracts

A Sensitive and Robust DNA Method for Authenticity Determination of Glehnia littoralis and Its Food Products

From DNA barcoding to nanoparticle-based colorimetric testing: a new frontier in cephalopod authentication

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