Development of a Species-Specific Coproantigen ELISA for Human Taenia solium Taeniasis

Taenia solium is endemic in most of the world, causing seizures and other neurological symptoms. Transmission is mainly maintained in rural areas by a human to pig cycle. Despite claims on its eradicability, sustainable interruption of transmission has not yet been reported. This manuscript reviews the conceptual basis for control, available diagnostic and control tools, and recent experiences on control in the field performed in Peru along the past decade

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“…10,11 Drawbacks of this technique include its costs, the need for an ELISA reader machine and reagents, and trained operators.…”

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confidence: 99%

Prevention and control ofTaenia soliumtaeniasis/cysticercosis in Peru

Gilman

González

Llanos-Zavalaga

et al. 2012

Pathogens and Global Health

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“…Recently, a T. solium-specific coproantigen enzyme-linked immunosorbent assay (ELISA) has been developed and shown to be potentially useful for mass screening (4). However, this test fails to identify T. saginata and T. asiatica taeniasis patients.…”

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confidence: 99%

Evaluation of a Loop-Mediated Isothermal Amplification Method Using Fecal Specimens for Differential Detection of Taenia Species from Humans

Nkouawa¹,

Sako²,

et al. 2010

J Clin Microbiol

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We compared the performance of loop-mediated isothermal amplification (LAMP) with that of a multiplex PCR method for differential detection of human Taenia parasites in fecal specimens from taeniasis patients. The LAMP method, with no false positives, showed a higher sensitivity (88.4%) than the multiplex PCR (37.2%). Thus, it is expected that the LAMP method has a high value for molecular diagnosis of taeniasis.Differential detection of Taenia species (Taenia saginata, T. asiatica, and T. solium) is a key point for control and prevention of taeniasis/cysticercosis in areas where Taenia disease is endemic. The identification of the carriers of T. solium tapeworms is most important for the prevention of cysticercosis, the most severe Taenia disease. Diagnosis of taeniasis by stool examination to detect taeniid eggs, the most common method, lacks both sensitivity and specificity because the eggs of Taenia species are morphologically indistinguishable. Moreover, Taenia species identification relying on comparative morphology of proglottids also lacks specificity.The coproantigen detection method has proved to be a useful application in epidemiological surveys (1, 5), but this method is genus specific, not species specific. Recently, a T. solium-specific coproantigen enzyme-linked immunosorbent assay (ELISA) has been developed and shown to be potentially useful for mass screening (4). However, this test fails to identify T. saginata and T. asiatica taeniasis patients. Therefore, molecular tools are more reliable for differential identifications of taeniid parasites. Several PCR technique-based detection methods for Taenia species in fecal samples, including the multiplex PCR method with mitochondrial DNA (18), the PCR-restriction fragment length polymorphism method with mitochondrial DNA (15, 16), and the nested-PCR method with the Tso31 gene encoding the T. solium oncosphere-specific protein (10), have been reported. We have recently developed a loop-mediated isothermal amplification (LAMP) method targeting cytochrome c oxidase subunit 1 (cox1) and cathepsin L-like cysteine peptidase (clp) genes for differential detection of Taenia species (13). This method utilizes a Bst DNA polymerase with strand replacement activity and four primers that recognize six sequences on the target DNA under isothermal conditions. This method has proved to be simple and highly sensitive and specific for differential detection of Taenia species by using DNA prepared from proglottids, cysticerci, and fecal samples of taeniasis patients (12) without using sophisticated and expensive equipment. In the present study, we evaluated its sensitivity and specificity with fecal specimens from taeniasis patients by comparison of the results obtained by the LAMP method with those obtained by the multiplex PCR method.Thirty-six fecal samples were collected in China from 26 T. saginata taeniasis patients, 5 T. asiatica taeniasis patients, and 5 T. solium taeniasis patients, and 7 fecal samples from Indonesia were obtained from T. saginata taeniasis p...

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“…Coproantigen method is usually only specific to the genus level, although species-specific assays for T. solium have been developed. 53 However, molecular methods can achieve species identification but they are not widely available.…”

Section: Diagnostic Methods and Impact On Epidemiologymentioning

confidence: 99%

Taenia solium Taeniasis and Cysticercosis in Southeast Asia

Aung¹,

Spelman²

2016

The American Society of Tropical Medicine and Hygiene

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Abstract. Human taeniasis/cysticercosis caused by the pork tapeworm Taenia solium has been identified as a potentially eradicable disease by the International Task Force for Disease Eradication of the World Health Organization. In southeast Asia, T. solium taeniasis/cysticercosis is considered one of the major neglected tropical diseases afflicting the region. In the last few decades, a considerable effort has been invested toward establishing the epidemiology and burden of disease in several southeast Asian countries. Moreover, further evidence is emerging as to understanding the dynamics of disease transmission and cultural, political, and socioeconomic factors influencing the success of control and eradication efforts within the region. However, despite major collaborations by several champion groups, advances have been slow and little remains known about the complete epidemiology of taeniasis/cysticercosis and the barriers to programmatic success. This review article aims to address the above issues with a further focus on the challenges to control and eradicate taeniasis/cysticercosis within the southeast Asia region.

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Development of a Species-Specific Coproantigen ELISA for Human Taenia solium Taeniasis

Cited by 68 publications

References 23 publications

Prevention and control ofTaenia soliumtaeniasis/cysticercosis in Peru

Prevention and control ofTaenia soliumtaeniasis/cysticercosis in Peru

Evaluation of a Loop-Mediated Isothermal Amplification Method Using Fecal Specimens for Differential Detection of Taenia Species from Humans

Taenia solium Taeniasis and Cysticercosis in Southeast Asia

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