Development of a simplified assay for the detection of neutralizing antibodies to Japanese encephalitis virus

Japanese encephalitis in a Finnish traveler on a two-week holiday in Thailand

“…Serum antibodies were controlled on day 13 showing titer elevation to 640 in IgM and IgG in JEV-IFA. The specificity of serum antibodies for JEV was confirmed by neutralization assays (Ting et al, 2001), where PRNT 80 (plaque reduction neutralization) titer to JEV was 1280, and no neutralization was observed against dengue virus type 2 in starting dilution of 1:40. The serum and CSF samples from day 3 after hospitalization were tested negative by virus isolation in mammalian and mosquito-cell cultures and JEV-specific reverse transcription-PCR (Trent and Chang, 1992).…”

Fatal dengue virus infection in a Finnish traveler

“…In a plaque reduction neutralization test (PRNT) modified from Ting et al (2001), the titers were higher towards DENV-1-4 (≥1:160) than towards JEV (<1:160) calculated as 80% reduction in plaque formation. However, no distinction between DENV serotypes could be made.…”

Impaired T Helper 1 Function of Nonstructural Protein 3–Specific T Cells in Japanese Patients with Encephalitis with Neurological Sequelae

“…Serum PRNT titers to JEV were estimated by a modified PRNT-ELISA [24] using JEV-specific hyperimmune mouse ascitic fluid (a kind gift of Dr. Dennis Trent, formerly at Centers for Disease Control and Prevention, Fort Collins, CO) and calculated using the standard formula of the National Institutes of Health, Japan [25]. Dengue virus-and West Nile virus-specific PRNT titer determinations were performed similarly.…”