Development of a simple pyrene-based ratiometric fluorescent chemosensor for copper ion in living cells

Fluorescent turn-on sensors based on pyrene-containing Schiff base derivatives for Cu2+ recognition: spectroscopic and DFT computational studies

“…25a,b The global analysis approach offers a more accurate determination of the equilibria involved since it avoids the manipulation of the actual experimental data to effect linear relationship was observed between the fluorescence intensity and the concentration of Cu 2+ showing a low detection limit of 8.80 × 10 -8 M. 26a This value is much lower than the concentration allowed in drinking water according to the US EPA (20 µM). 20 Furthermore, when excited at 367 nm, the quantum yield, Φ, of the L1-Cu 2+ complex was 0.31 which was 31-fold more enhanced than of free ligand L1 alone. 26b The fluorescence titration experiments of Cu 2+ complex was 0.31 which was 31-fold more enhanced than of free ligand L1 alone.…”

“…19 Based on monomer-excimer conversion, a pyrene chemosensor containing a thiophene moiety was a highly selective and ratiometric fluorescent sensor which induces the dual appearance of excimer emission and disappearance of monomer emission after addition of Cu 2+ ion. 20 Venkatesan and Wu. have designed a pyrene-based fluorescent probe bearing the hydrazinyl pyridine moiety for Cu 2+ ion detection based on the PET mechanism. The chemosensors binding with the metal ion block the PET mechanism resulting in significant fluorescence enhancement.…”

Pyrene Excimer-Based Fluorescent Labeling of Cysteines Brought into Close Proximity by Protein Dynamics: ASEM-Induced Thiol-Ene Click Reaction for High Spatial Resolution CLEM

“…For example, pyrene was conjugated with DNA to detect antisense nucleic acids [ 44 ]. Furthermore, it was used as a chemosensor for ATP, metal ions, and pH [ 42 , 49 , 50 , 51 ].…”

A novel hexahydroquinazolin-2-amine-based fluorescence sensor for Cu2+ from isolongifolanone and its biological applications

“…A uorescence probe is a simple, fast, and sensitive way of detecting copper ions compared with traditional methods such as electrochemistry, 16 spectrophotometry, 17 voltammetry, 18 and atomic absorption spectroscopy. 19 Many sensitive uorescence probes for Cu 2+ have been reported, such as ferrocenyl, 20 benzimidazole, 21 benzothiazole, 22,23 pyrene, 24,25 BODIPY, 26,27 rhodamine, [28][29][30][31] etc. Pyrimidine is a typical nitrogen heterocyclic compound and has many structure sites that can be modied.…”