Development of a simple multiresidue extraction method for the quantification of a wide polarity range list of pesticides and transformation products in eggs by liquid chromatography and tandem mass spectrometry

Abstract

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Miniaturization of an extraction protocol for the monitoring of pesticides and polar transformation products in biotic matrices

“…The initial protocol (IP), based on a previous work by Dufour et al (2020) and designed for the analysis of pesticides and their degradation products in high-mass environmental samples (e.g., eggs), is illustrated in Fig. 1.…”

“…The development of the LC-MS/MS method was described in Dufour et al (2020). Analysis of the 41 PPP residues was performed using a 1290 Infinity UPLC system (Agilent Technologies, Massy, FR) coupled with a 5500 QTRAP mass spectrometer (Sciex, Villebon-sur-Yvette, FR) in both positive and negative modes using electrospray ionization.…”

“…This variability tended to decrease for higher concentration levels as the inter-day precision was lower than or equal to 20% for 31 molecules at C4, which is in agreement with European guidelines (European Commission DG-SANTE 2019). Moreover, if we leave aside the nature of the tested matrix, the inter-day precision is better with IP (Dufour et al, 2020) than with MP. This was most probably due to the decrease of the sample mass which led to an increase of the measurement uncertainties, as shown by Han et al (2018) and Lehotay et al (2018) on food samples towards pesticide contaminations.…”

“…Indeed, they are more soluble in the aqueous phase than in acetonitrile, even if the latter is saturated with buffer, resulting in low or no recoveries. (Dufour et al 2020).…”

“…Providing a robust, reliable, and sensitive analytical method based on low matrix masses could increase the number of studies in that research field and benefit the understanding of the global dynamics of organic micropollutants in the environment. In this context, the aim of this work was to study the effect of the miniaturization of a previously developed extraction protocol (Dufour et al, 2020) on analytical performance and to test its applicability on two matrices: fish and MIs. As masses of MI samples are very low, the effect of miniaturization was first investigated on fish, and then applied and validated on MIs.…”