Development of a simple estimation method of serum caffeine concentration using a point-of-care test kit for urinary caffeine
Kenji Tsujikawa,
Yuki Okada,
Hiroki Segawa
et al.
Abstract:Purpose
Serum caffeine concentration is an indicator of caffeine intoxication; however, it is difficult to measure it in most emergency departments. We developed a simple estimation method using a point-of-care test kit for urinary caffeine.
Methods
Caffeine-spiked human serum (100, 50, 25, and 10 µg/mL) was diluted 10-, 20-, 50-, and 100-fold with phosphate-buffered saline and applied to the kit. After 5 min incubation, the kit was scanned by a flatbed sc… Show more
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