Development of a simple bio-hydrogen production system through dark fermentation by using unique microflora

Fermentative Hydrogen Production by Molasses; Effect of Hydraulic Retention Time, Organic Loading Rate and Microbial Dynamics

“…The bacterial population examination confirmed in this case a symbiosis between the lactic acid and hydrogen producing bacteria, too. In another work (Ohnishi et al, 2010), in which kitchen waste containing lactate was used as substrate, lactic acid removal was also observed along with carbohydrate degradation during hydrogen production in an Anaerobic Sequencing Batch Reactor. The phylogenetic analysis affiliated the dominant bacteria to the genera of Lactobacillus, Selonomonas, Veillonella and Megasphaera, which belong to the phylum of Firmicutes, to Prevotella genus of the phylum of Bacteroidetes and to Atopobium and Bifidobacterium of the Actinobacteria phylum.…”

Examination and Comparison of Microbial Diversity in Field-Scale Sewage Sludge Composters

“…DNA extraction from each isolate was performed using the bead beating method (Ohnishi et al, 2010). After cells were grown for 24 h, they were suspended in 1 ml sterile distilled water www.intechopen.com Changing Diversity in Changing Environment 4 by using a sterile swab, and centrifuged for 5 min (12,000 g, 4 °C).…”

Development of a 16S rRNA Gene Primer and PCR-Restriction Fragment Length Polymorphism Method for Rapid Detection of Members of the Genus Megasphaera and Species-Level Identification

“…M. cerevisiae, M. paucivorans, and M. sueciensis are regarded as obligate, beer spoilage bacteria (1,6). M. elsdenii is a normal inhabitant of the gastrointestinal tract in mammals such as humans and cattle (3)(4)(5) and is a useful hydrogen producer in a very simple biohydrogen production system (8). Biohydrogen figures prominently in the solution of future energy problems, because hydrogen is an inexhaustible fuel and produces only water as a combustion product (12).…”