Development of a Simple and Efficient Method for Transformation of Buckwheat Plants (<i>Fagopyrum esculentum</i>) Using<i>Agrobacterium tumefaciens</i>

Kojima, Mineo; Arai, Yukie; Iwase, Narumi; Shirotori, Kimiko; Shioiri, Hidenari; Nozue, Masayuki

doi:10.1271/bbb.64.845

Cited by 39 publications

(20 citation statements)

References 3 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It would have been easier to determine false positive results, if the vector sequence contained marker genes from more than one organism. However, pBI121 is a popular binary vector and has recently been used by researchers for several plants including peanut (Rohini and Rao 2001;Sharma and Anjaiah 2000), shallot (Zheng et al 2001), spruce (Le et al 2001), buckwheat (Kojima et al 2000), poppy (Park and Facchini 2000) and pine (Tang 2001). pBI121 has also been used for in planta transformation of cactus (Seol et al 2008).…”

Section: Discussionmentioning

confidence: 99%

“…Electroporation-mediated gene transfer into intact meristems in planta and a variety of pollen transformation procedures have also been reported (Bent 2000). Techniques based on similar principles have been applied to other plant species, including Arabidopsis (Clough and Bent 1998), Triticum aestivum L. (Supartana et al 2006), Fagopyrum esculentum (Kojima et al 2000), Morus alba L. (Ping et al 2003), Hibiscus cannabinus var. aokawa No.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Tissue culture independent transformation for Corchorus olitorius

Sajib

Islam

Reza

et al. 2008

Plant Cell Tiss Organ Cult

View full text Add to dashboard Cite

In vitro regeneration is difficult for species of Corchorus and several transformation attempts based on tissue culture have failed. We describe a successful transformation protocol for C. olitorius using a technique independent of tissue culture. Primary growth of a plant is brought about from the activities of meristematic region and subsequent division and differentiation of the derivative cells into the tissues and organs of the plant. The principal behind the current study was that if some dividing cells in the meristematic region become transformed by Agrobacterium and the cells retain the capacity for cell division, then the transgene(s) will be transferred to progeny cells, and if some of these cells later differentiate to form floral buds, seeds generated from these buds will inherit the transgene(s) to next generation. In this study young jute plants were transformed at shoot apical meristematic region using Agrobacterium tumefaciens. Heritable transmission of the transgene to progeny from genetically modified plants was confirmed by gus gene expression by histochemical analysis, selection on kanamycin containing medium, RT-PCR, PCR amplification and Southern hybridization. Efficiency of transformation was determined by selection on medium containing kanamycin, and inheritance of transgene to T 2 generation plants.

show abstract

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Tissue culture independent transformation for Corchorus olitorius

Sajib

Islam

Reza

et al. 2008

Plant Cell Tiss Organ Cult

View full text Add to dashboard Cite

show abstract

“…Zone C 100 mg of pistil filaments, seed-buds, or embryos as described by Kojima et al (2000). Expression of the gus reporter gene.…”

Section: Treatment Zone (A) 10 CMmentioning

confidence: 99%

“…In order to obtain genetically modified plants, a variety of methods are used in laboratory practice for agrobacterial transformation of vegetative and generative organs and tissues (see reviews : Chumakov et al, 2006;Chumakov and Moiseeva, 2012;Mehrotra and Goyal, 2012). Thus, leaf disks and protoplasts (Draper et al, 1991), root fragments (Zambre et al, 2003), callus (Danilova and Dolgikh, 2003), seeds (Feldmann and Marks, 1987;Stepanova et al, 2006), and meristematic tissues of germinating seeds (Kojima et al, 2000) have been used for the transformation of vegetative cells. For the transformation of plant generative cells, the floral dip method was proposed, which is based on immersion of Arabidopsis pistillate flowers and pollen suppliers into a suspension of agrobacteria with activated virulence genes (Clough and Bent, 1998;Bent, 2006), as well as application of an agrobacterial suspension to wheat ears (Hess et al, 1990;Pukhal'skii et al, 1996) and pollen transformation (Hess et al, 1987;Harwood et al, 1996;Eapen, 2011).…”

mentioning

confidence: 99%

Analysis of proliferation and survival of agrobacteria after inoculation of maize pistil filaments

et al. 2016

View full text Add to dashboard Cite

While the authors have previously developed a method of pistil filament treatment with Agrobacterium cells during blossoming for the transformation of maize generative cells, the mechanism for bacterial T-DNA penetration into the embryo sac remained unknown. This article analyzes the possibility of agrobacterial penetration into the maize embryo via pollen tubes. Microbiological, PCR, and GUS techniques were used to confirm that agrobacteria could spread for up to 20 cm from the site of inoculation and were detected in maize embryo tissues as early as 24 h after inoculation, while they were not revealed after 5-13 days.

show abstract

“…Inplanta transformation techniques have been succeeded in buckwheat plant (Fagopyrum esculentum M.), mulberry (Morus alba L.) and kenaf (Hibiscus cannabinus L.) (Ping et al 2003;Kojima et al 2004). The transformation efficiency of all plants transformed by such method was extremely high compared with the general in vitro transformation method (Kojima et al 2000).…”

Section: Introductionmentioning

confidence: 99%

Agrobacterium tumefaciens-MEDIATED IN-PLANTA TRANSFORMATION OF INDONESIAN MAIZE USING pIG121Hm-Cs PLASMID CONTAINING nptII AND hpt GENES

Listanto¹,

Riyanti²,

Sustiprijatno³

2017

Indones. J. Agric. Sci.

View full text Add to dashboard Cite

Maize (Zea mays L.) productivity in Indonesia is challenged to be increased using genetic engineering. Recent advances in Agrobacterium tumefaciens-mediated in-planta transformation makes it possible to transform maize with low cost, and simple method. This study aimed to confirm pIG121Hm-Cs plasmid in A. tumefaciens, and to estimate the efficiency level of A. tumefaciens-mediated in-planta transformation of Indonesian maize by using pIG121Hm-Cs plasmid containing nptII and hpt genes. A series of studies were conducted including confirmation of gene construct of pIG121Hm-Cs plasmid in A. tumefaciens, transformation of four maize lines through A. tumefaciens-mediated in-planta technique, acclimatization of transformant plants and molecular analysis of selected plants using polymerase chain reaction (PCR). The pIG121Hm-Cs plasmid was confirmed via PCR analysis using specific primers of nptII and hpt genes and resulted 700 bp and 500 bp for fragments of nptII and hpt, respectively. After selection, acclimatization and molecular analysis steps, the efficiency levels of transformation of four maize lines were low, ranging from 3.8% to 12.8%. The level of transformation efficiency of ST-27 line was the highest accounting for 12.8% of 45 planted embryos on selection medium based on PCR analysis using specific primer for nptII gene. Overall, A. tumefaciensmediated in planta transformation on maize floral pistil in this study proved to be successful and rapid. Therefore, this enhanced transformation method will be beneficial for Indonesian maize genetic engineering.

show abstract

Development of a Simple and Efficient Method for Transformation of Buckwheat Plants (Fagopyrum esculentum) UsingAgrobacterium tumefaciens

Cited by 39 publications

References 3 publications

Tissue culture independent transformation for Corchorus olitorius

Tissue culture independent transformation for Corchorus olitorius

Analysis of proliferation and survival of agrobacteria after inoculation of maize pistil filaments

Agrobacterium tumefaciens-MEDIATED IN-PLANTA TRANSFORMATION OF INDONESIAN MAIZE USING pIG121Hm-Cs PLASMID CONTAINING nptII AND hpt GENES

Contact Info

Product

Resources

About