Development of a simple and sensitive HPLC–UV method for the simultaneous determination of cannabidiol and Δ9-tetrahydrocannabinol in rat plasma

Zgair, Atheer; Wong, Jonathan; Sabri, Akmal Hidayat Bin; Fischer, Peter M.; Constantinescu, Cris S.; Gershkovich, Pavel

doi:10.1016/j.jpba.2015.05.019

Cited by 60 publications

(59 citation statements)

References 50 publications

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“…Table presents the summary of all LC‐based analytical methods for the quantification of cannabinoids in various animal samples published during 2010–2019 . All those LC methods were HPLC‐based, and there has been no report on the use of UPLC for animal sample during this period.…”

Section: Hplc and Uplc (Or Uhplc) Analysis Of Naturally Occurring Canmentioning

confidence: 99%

A review on the recent advances in HPLC, UHPLC and UPLC analyses of naturally occurring cannabinoids (2010–2019)

Nahar

Önder

Sarker

2019

Phytochemical Analysis

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Introduction Organic molecules that bind to cannabinoid receptors are called cannabinoids, and they have similar pharmacological properties like the plant, Cannabis sativa L. Hyphenated liquid chromatography (LC), incorporating high‐performance liquid chromatography (HPLC) and ultra‐performance liquid chromatography (UPLC, also known as ultrahigh‐performance liquid chromatography, UHPLC), usually coupled to an ultraviolet (UV), UV‐photodiode array (PDA) or mass spectrometry (MS) detector, has become a popular analytical tool for the analysis of naturally occurring cannabinoids in various matrices. Objective To review literature on the use of various LC‐based analytical methods for the analysis of naturally occurring cannabinoids published since 2010. Methodology A comprehensive literature search was performed utilising several databases, like Web of Knowledge, PubMed and Google Scholar, and other relevant published materials including published books. The keywords used, in various combinations, with cannabinoids being present in all combinations, in the search were Cannabis, hemp, cannabinoids, Cannabis sativa, marijuana, analysis, HPLC, UHPLC, UPLC, quantitative, qualitative and quality control. Results Since 2010, several LC methods for the analysis of naturally occurring cannabinoids have been reported. While simple HPLC‐UV or HPLC‐UV‐PDA‐based methods were common in cannabinoids analysis, HPLC‐MS, HPLC‐MS/MS, UPLC (or UHPLC)‐UV‐PDA, UPLC (or UHPLC)‐MS and UPLC (or UHPLC)‐MS/MS, were also used frequently. Applications of mathematical and computational models for optimisation of different protocols were observed, and pre‐analyses included various environmentally friendly extraction protocols. Conclusions LC‐based analysis of naturally occurring cannabinoids has dominated the cannabinoids analysis during the last 10 years, and UPLC and UHPLC methods have been shown to be superior to conventional HPLC methods.

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Section: Hplc and Uplc (Or Uhplc) Analysis Of Naturally Occurring Canmentioning

confidence: 99%

A review on the recent advances in HPLC, UHPLC and UPLC analyses of naturally occurring cannabinoids (2010–2019)

Nahar

Önder

Sarker

2019

Phytochemical Analysis

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“…This makes THC a drug of abuse and correct identification of this compound is thus important from a forensic point of view. The two cannabinoids, can be separated using chromatographic or electrophoretic methods, such as planar chromatography [35], LC-UV [36], LC-MS(MS) [37,38], CE-LED-induced fluorescence detection [39], which require a considerable analysis time (5-30 min). In the present study, we studied the potential of TIMS for the fast separation of THC and CBD.…”

Section: Separation Of Isomers Employing Cation Adduct Formationmentioning

confidence: 99%

Adduct-ion formation in trapped ion mobility spectrometry as a potential tool for studying molecular structures and conformations

Zietek

Mengerink

Jordens

et al. 2017

Int. J. Ion Mobil. Spec.

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Recent developments in the field of ion mobility spectrometry provide new possibilities to explore and understand gas-phase ion chemistry. In this study, hyphenated trapped ion mobility spectrometry-mass spectrometry (TIMS-MS) was applied to investigate analyte ion mobility as function of adduct ion formation for twelve pharmaceutically relevant molecules, and for tetrahydrocannabinol (THC) and its isomer cannabidiol (CBD). Samples were introduced by direct infusion and ions were generated with positive electrospray ionization (ESI+) observing protonated and sodiated ions. Measurements were performed with and without addition of cesium-, lithium-, silver-and sodium ions to the samples. For the tested compounds, metal adduct ions with the same m/z but with different mobility and collision cross section (CCSs) were observed, indicating different molecular conformations. Formation of analyte dimers was also observed, which could be associated with molecular geometry of the compounds. By optimizing the range and speed of the electric field gradient and ramp, respectively, the separation of THC and CBD was achieved by employing the adduct formation. This study demonstrates that the favorable resolution of TIMS combined with the ability to detect weakly bound counter ions is a valuable means for rapid detection, separation and structural assignment of molecular isomers and analyte conformations.

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“…3 Many small-molecule pesticides have known or suspected toxic health effects, including a variety of neurotoxic effects. 4 Currently used methods for pesticide detection include gas chromatography-mass spectrometry (GC-MS), 5 liquid chromatography-mass spectrometry (LC-MS), 6 and surface-enhanced Raman spectroscopy (SERS). 7 Fluorescence-based detection systems, including molecularly imprinted polymers, 8 gold nanoparticles, 9 and fluorescent polymers 10 have also been developed, and in many cases lead to more sensitive detection limits and faster response times.…”

Section: Rapid and Efficient Pesticide Detection Via Cyclodextrinprommentioning

confidence: 99%

Rapid and efficient pesticide detection via cyclodextrin-promoted energy transfer

Serio

Roque

Badwal

et al. 2015

Analyst

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Development of a simple and sensitive HPLC–UV method for the simultaneous determination of cannabidiol and Δ9-tetrahydrocannabinol in rat plasma

Cited by 60 publications

References 50 publications

A review on the recent advances in HPLC, UHPLC and UPLC analyses of naturally occurring cannabinoids (2010–2019)

A review on the recent advances in HPLC, UHPLC and UPLC analyses of naturally occurring cannabinoids (2010–2019)

Adduct-ion formation in trapped ion mobility spectrometry as a potential tool for studying molecular structures and conformations

Rapid and efficient pesticide detection via cyclodextrin-promoted energy transfer

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