Development of a sequence characterized amplified region (SCAR) marker associated with high rooting ability in Larix

Abstract

In this study, bulked segregant analysis (BSA) was used on Larix leptolepis × Larix olgensis hybrids to identify a random amplified polymorphic DNA (RAPD) marker associated with high rooting ability in larch. Two DNA bulks: H (high rooting ability) bulk and L (low rooting ability) bulk were constructed according to the rooting percentages of the stock plants. Among the 328 primers, only S356 could amplify a specific band, named S356 445 , which only existed in the H bulk and was further confirmed following selective genotyping of individual hybrids. Grounded on the border sequences, S356 445 was converted to a sequence characterized amplified region (SCAR) marker, HRL445，which can be useful in marker-assisted selection (MAS) to screen for larch with high rooting ability. All the results strongly indicated that S356 445 and HRL445 were closely associated with high rooting ability in larch.

2D random walk representation of Begonia × tuberhybrida multiallelic loci used for germplasm identification

“…Therefore, it is of the general ⎯⎯⎯⎯ economic interest to understand the hidden nature of marker polymorphism (Li et al 2008). Especially, the question frequently arises about how genetic characteristics of marker loci like their observed heterozygosity (H o ) or allelic composition might be related to their DNA primary sequence.…”

Development of dominant sequence characterized amplified region (SCAR) marker linked with plume moth (Exelastis atomosa Walsingham 1886) resistance in pigeon-pea

“…Hence, the linked/ co-segregated RAPD markers were converted into SCAR marker based on sequence data prior to its introduction in crop breeding efforts. Literature evinced on development good many number of SCAR markers for effective application in mapping of genes agro-economic traits including biotic stress resistance in various crops, vis-a-vis their employment in marker assisted selection (MAS) programs (Gupta et al, 2006a;2006b;Li et al, 2008;Dianese et al, 2010;. However, there is no report available on development of SCAR marker and its application in pigeon-pea.…”