Development of a sensor-array chip with immobilized antibodies and the application of a wireless antigen-screening system

Isoda, Takaaki; Urushibara, I.; Satō, Masayuki; Uemura, Hirotsugu; Sato, Hikaru; Yamauchi, Noriyoshi

doi:10.1016/j.snb.2007.10.022

Cited by 9 publications

(14 citation statements)

References 22 publications

(29 reference statements)

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“…An ionic layer, like an electric double layer, forms at the surface of the solids and liquids [10]. When a solution containing metal ions with a concentration N is deposited onto the surface of an organic insulator with an adsorbent an electric double layer forms at the boundary surface(Figure 1(a)).…”

Section: Methodsmentioning

confidence: 99%

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Evaluation of Complexation Ability Using a Sensor Electrode Chip Equipped with a Wireless Screening System

Isoda

Urushibara²,

Sato³

et al. 2012

Sensors

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We fabricated an electrode chip with a structure coated by an insulation layer that contains dispersed SiO2 adsorbent particles modified by an amino-group on a source-drain electrode. Voltage changes caused by chelate molecule adsorption onto electrode surfaces and by specific cation interactions were investigated. The detection of specific cations without the presence of chelate molecules on the free electrode was also examined. By comparing both sets of results the complexation ability of the studied chelate molecules onto the electrode was evaluated. Five pairs of source-drain electrodes(×8 arrays) were fabricated on a glass substrate of 20 × 30mm in size. The individual Au/Cr (1.0/0.1μm thickness) electrodes had widths of 50 μm and an inter-electrode interval of 100μm.The fabricated source-drain electrodes were further coated with an insulation layer comprising a porous SiO2 particle modified amino-group to adsorb the chelate molecules. The electrode chip was equipped with a handy-type sensor signal analyzer that was mounted on an amplifier circuit using a Miniship™ or a system in a packaged LSI device. For electrode surfaces containing different adsorbed chelate molecules an increase in the sensor voltage depended on a combination of host-guest reactions and generally decreased in the following order:5,10,15,20-tetrakis(N-methylpyridinium-4-yl)-21H,23H-porphine, tetrakis(p-toluenesulfonate) (TMPyP)as a Cu2+chelator and Cu2+>2-nitroso-5-[N-n-propyl-N-(3-sulfopropyl)amino]phenol(nitroso-PSAP) as an Fe2+chelator and Fe2+>4,7-diphenyl-1,10-phenanthrolinedisulfonic acid, disodium salt (BPDSA) as an Fe2+chelatorand Fe2+>3-[3-(2,4-dimethylphenylcarbamoyl)-2-hydroxynaphthalene-1-yl-azo]-4-hydroxybenzenesulfonic acid, sodium salt (XB-1) as a Mg2+chelator and Mg2+>2,9-dimethyl-4,7-diphenyl-1,10-phenanthrolinedisulfonic acid, disodium salt (BCIDSA) as a Cu2+chelator and Cu2+, respectively. In contrast, for the electrode surfaces with adsorbed O,O′-bis(2-aminoethyl)ethyleneglycol-N,N,N′,N′-tetraacetic acid (GEDTA) or O,O′-bis(2-aminophenyl)ethyleneglycol-N,N,N′,N′-tetraacetic acid, tetrapotassium salt, hydrate (BAPTA) as a Ca2+chelator no increase in the detection voltage was found for all the electrode tests conducted in the presence of Ca2+.To determine the differences in electrode detection, molecular orbital (MO) calculations of the chelate molecules and surface molecular modeling of the adsorbents were carried out. In accordance with frontier orbital theory, the lowest unoccupied MO (LUMO) of the chelate molecules can accept two lone pair electrons at the highest occupied MO (HOMO) of the amino group on the model surface structure of the SiO2 particle. As a result, a good correlation was obtained between the LUMO-HOMO difference and the ion response of all the electrodes tested. Based on the results obtained, the order of adsorbed chelate molecules on adsorption particles reflects the different metal ion detection abilities of the electrode chips.

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Section: Methodsmentioning

confidence: 99%

“…It was investigated for the detection of antigens in a phosphoric acid buffer solution(PBS)[10,11]. The source-drain electrode was fabricated on a chip and the insulator layer was composed of a novolac resin that was deposited onto SiO 2 particles as the adsorbent on the electrode pair.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Complexation Ability Using a Sensor Electrode Chip Equipped with a Wireless Screening System

Isoda

Urushibara²,

Sato³

et al. 2012

Sensors

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“…(7) We previously reported a chip-mounted microelectrode composed of source and drain electrodes coated with an insulator layer and adsorbent particles to immobilize antibody molecules for the detection of antigens in solution. (8,9) The source and drain electrodes were fabricated on a chip; the insulator layer was composed of a novolac resin that was deposited onto SiO 2 particles modified with aminoalkyl groups as the adsorbent on the electrodes. Antibody molecules were quickly immobilized onto the aminated SiO 2 particles by means of various adsorption phenomena.…”

Section: Introductionmentioning

confidence: 99%

Development of Wireless Electrical Conductivity Sensor Screening System to Evaluate Protein Binding to Sensor Films

2017

Sensors and Materials

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We developed a wireless electrical conductivity (EC) sensor screening system to evaluate changes in the EC of sensor films. This sensor system was designed to be equipped with a special sensor chip (14 × 40 mm 2 ), and changes in the EC of a droplet containing a sensor film (ϕ3 mm) on the chip were determined. Electrode pairs were fabricated on glass substrates by photolithography, and a well structure was formed around each electrode pair using photoreactive resin. This sensor system achieved parallel detection of EC signals from five wells, each containing 5 μl of solution with a sensor film in 10 s (N = 5). Furthermore, we developed sensor films that displayed the changes in the EC upon the adsorption and/or chemical binding of protein with Na + as a counterion. To form the sensor films, the surface of cellulose was chemically modified with alkylamino groups. The EC detected the sensor screening system using aminated cellulose with physically adsorbed bovine serum albumin (BSA) and the amount of Na + ions increased linearly with BSA concentration with a correlation coefficient (R 2 ) of 0.87. Meanwhile, aminated cellulose with chemically bound BSA through polylysine and glutaraldehyde and adsorbed Na + exhibited an EC that depended on the BSA concentration, with an R 2 value of 0.943. The detection limit of BSA was 10 ng/ml for both types of sensor film. On the basis of our results, the mechanism behind the increased sensitivity of the sensor films with immobilized protein in the presence of a counterion was proposed.

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“…We previously reported on the standard properties of a biosensor that had been mounted on a chip with a source-drain electrode, coated with an insulating layer of adsorbent particles for immobilizing antibody molecules. (16) Figure 1(a) shows a schematic structure of a sensor-array chip. When a liquid is deposited on the surface of an insulator, an electric double layer occurs at the boundary surface; the insulator molecules become polarized, resulting in the formation of an opposing charge on the boundary surface between the liquid and the solid, and the reverse side of the insulator.…”

Section: Introductionmentioning

confidence: 99%

“…On the basis of this principle, a sourcedrain electrode produces a signal in response to changes in current originating from the antigen-antibody reactions, such as rat anti-IgE and IgE, leukotriene B4 and antileukotriene B4, and biotin maleimide and avidin, at the boundary surface of the insulator layer. (16) In the next stage of sensor development, the sensor-array chip with immobilized antibodies proposed previously will be applied to measure the concentrations of immunoglobulins. Accuracy, as well as repeatability for sensor measurement, will be improved if a state of polarization of all sensor arrays can be controlled equally by adding an insulating layer on the electrode.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Immunoglobulin Sensing Function Using a Fullerene-Composite-Polymer-Coated Sensor Electrode

2011

Sensors and Materials

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The detection of antigen-antibody reactions in normal saline solution measured using a chip-mounted source-drain electrode coated with a fullerene composite polymer layer containing an immobilized immunoglobulin G (IgG) molecule was investigated as a function of anti-IgG concentration. Five pairs of source-drain electrodes (5 wells) were fabricated on a glass substrate (16 mm × 40 mm), in which the individual Au/Cr (1.0/0.1 μm thickness) sensors had widths of 0.5 mm and an interelectrode interval of 1.5 mm. The fabricated source-drain electrodes were further coated with an insulation layer comprising porous materials for use as an adsorbent for receiving IgG molecules. The sensor chip was equipped with a sensor signal analyzer that comprised either an amplifier circuit with a Miniship TM or a system in a packaged large-scale integration (LSI) device. Accuracy, as well as reproducibility for the detection of anti-IgG, was improved by the addition of fullerene to the insulation layer, which had a correlation coefficient for the calibration curve of 0.83-0.88, whereas a fullerene-free insulation layer had a low correlation coefficient between 0.16-0.38.

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Development of a sensor-array chip with immobilized antibodies and the application of a wireless antigen-screening system

Cited by 9 publications

References 22 publications

Evaluation of Complexation Ability Using a Sensor Electrode Chip Equipped with a Wireless Screening System

Evaluation of Complexation Ability Using a Sensor Electrode Chip Equipped with a Wireless Screening System

Development of Wireless Electrical Conductivity Sensor Screening System to Evaluate Protein Binding to Sensor Films

Evaluation of Immunoglobulin Sensing Function Using a Fullerene-Composite-Polymer-Coated Sensor Electrode

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