The nanofabrication of composite-based sensors can be seen in the research literature, but high price restricts their use on a wider scale in the developing world. Here, we report their use for the detection of thiourea (TU), a well-known carcinogen. Briefly, reduced graphene (r-GO) sheets with silver (Ag) nanoparticles impregnated were prepared via hydrothermal and chemical reduction method. The synthesized nano-composite material was characterized by various physiochemical techniques like Fourier transform infrared spectroscopy, X-ry diffraction, scanning electronmicroscopy, and cyclic voltammetry (CV) and applied as synthesized for the detection and sensing of TU. The Ag-rGO changed electrode enhanced approximately three times greater anodic current compared to rGO and five times greater anodic current compared to GCE. Electrical and catalytic oxidation of TU was achieved by GCE, GO, rGO, and Ag-rGO at 0.2 to 0.25 V applying CV. The concentrations used were 10 to 50 µM, while the current at anode increased as the concentration increased. The anodic current is increased by an increasing scan rate of 20, 40, 60, 80, and 100 mV/s, so it could be used for recognition of TU in real time sample with good sensitivity, stability and reproducibility.