Development of a sensitive molecular detection assay for mango malformation disease caused by Fusarium mangiferae

Wu, Jingbo; Liu, Feng; Zhan, Rulin; Li, Guoping; Zhao, Yan‐Long; Jin-mei, Chang; He, Yuanhuizi

doi:10.1007/s10529-016-2048-9

Cited by 6 publications

(6 citation statements)

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“…In the past decade, MMD has destroyed many thousands of hectares of mango in tropical and subtropical countries. Because of the economic importance of MMD, many studies have been performed on the occurrence (Steenkamp et al, 2000 ; Lima et al, 2009 ; Iqbal et al, 2011b ), pathogen genetic diversity (Iqbal et al, 2006 ; Liu et al, 2014 ), pathogen detection (Wu et al, 2016 ), pathogen cytology (Iqbal et al, 2010 ), infection life cycle (Freeman et al, 1999 ; Gamliel-Atinsky et al, 2009 ), and chemical control (Iqbal et al, 2011a ) of the disease, but research on the screening of mango germplasm for MMD resistance and on molecular mechanisms underlying MMD resistance and the pathogenicity of F. mangiferae is scarce (Singh, 2006 ). The main control measures against MMD include destruction of diseased mango branches, use of disease-free plant materials and fungicides.…”

Section: Introductionmentioning

confidence: 99%

Transcription Profiling Analysis of Mango–Fusarium mangiferae Interaction

Liu

Zhan

et al. 2016

Front. Microbiol.

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Malformation caused by Fusarium mangiferae is one of the most destructive mango diseases affecting the canopy and floral development, leading to dramatic reduction in fruit yield. To further understand the mechanism of interaction between mango and F. mangiferae, we monitored the transcriptome profiles of buds from susceptible mango plants, which were challenged with F. mangiferae. More than 99 million reads were deduced by RNA-sequencing and were assembled into 121,267 unigenes. Based on the sequence similarity searches, 61,706 unigenes were identified, of which 21,273 and 50,410 were assigned to gene ontology categories and clusters of orthologous groups, respectively, and 33,243 were mapped to 119 KEGG pathways. The differentially expressed genes of mango were detected, having 15,830, 26,061, and 20,146 DEGs respectively, after infection for 45, 75, and 120 days. The analysis of the comparative transcriptome suggests that basic defense mechanisms play important roles in disease resistance. The data also show the transcriptional responses of interactions between mango and the pathogen and more drastic changes in the host transcriptome in response to the pathogen. These results could be used to develop new methods to broaden the resistance of mango to malformation, including the over-expression of key mango genes.

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Section: Introductionmentioning

confidence: 99%

Transcription Profiling Analysis of Mango–Fusarium mangiferae Interaction

Liu

Zhan

et al. 2016

Front. Microbiol.

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“…Malformation of mango inflorescences (MMI) threatens mango productivity and causes significant damage to the mango industry 25,36 . Although Fusarium species have been implicated in malformation, the etiology of this disease remains obscure and effective control measures have not yet been identified 4,11,25 . Moreover, fungicides and other tested chemicals have not proved effective in controlling the disease 37,38 .…”

Section: Discussionmentioning

confidence: 99%

“…Previously, we have reported higher content of ethylene in MMI 7,8 . In addition to producing ethylene 9 , Fusarium mangiferae leads to the overexpression of key mango genes 10,11 , which affects plant hormone homeostasis 12 and results in stress hormone ethylene formation 7 . Cyanide, a co-product of ethylene synthesis exerts adverse, toxic and long-term effects on plant growth and development 13,14 .…”

Section: Introductionmentioning

confidence: 99%

Cyanide produced with ethylene by ACS and its incomplete detoxification by β-CAS in mango inflorescence leads to malformation

Ansari

Kaushik

Bains

et al. 2019

Sci Rep

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Malformation of mango inflorescences (MMI) disease causes severe economic losses worldwide. Present research investigates the underlying causes of MMI. Results revealed significantly higher levels of cyanide, a by-product of ethylene biosynthesis, in malformed inflorescences (MI) of mango cultivars. There was a significant rise in ACS transcripts, ACS enzyme activity and cyanide and ethylene levels in MI as compared to healthy inflorescences (HI). Significant differences in levels of methionine, phosphate, S-adenosyl-L-methionine, S-adenosyl-L-homocysteine, ascorbate and glutathione, and activities of dehydroascorbate reductase and glutathione reductase were seen in MI over HI. Further, a lower expression of β-cyanoalanine synthase (β-CAS) transcript was associated with decreased cellular β-CAS activity in MI, indicating accumulation of unmetabolized cyanide. TEM studies showed increased gum-resinosis and necrotic cell organelles, which might be attributed to unmetabolized cyanide. In field trials, increased malformed-necrotic-inflorescence (MNI) by spraying ethrel and decreased MNI by treating with ethylene inhibitors (silver and cobalt ions) further confirmed the involvement of cyanide in MMI. Implying a role for cyanide in MMI at the physiological and molecular level, this study will contribute to better understanding of the etiology of mango inflorescence malformation, and also help manipulate mango varieties genetically for resistance to malformation.

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“…This technique was first demonstrated useful for measuring genetic diversity in plants and animals by Zietkiewicz et al (1994), and then shown to be useful for analysing the genetic diversity of plant-pathogenic fungi as well (Hannachi, Poli, Rezgui, Prassad, & Cherif, 2015;Menzies, Bakkeren, Matheson, Procunier, & Woods, 2003;Rampersad, 2013). Moreover, ISSR markers can be further converted into the more specific sequence-characterized amplified region (SCAR) markers (Aggarwal, Gupta, Banerjee, & Singh, 2011;Gao, Chen, & Liu, 2011;Wu et al, 2016). The species-specific SCAR markers have been successfully developed for identifying phytopathogenic fungi, such as Bipolaris sorokiniana (Aggarwal et al, 2011), Tilletia controversa (Gao et al, 2011) and Fusarium mangiferae (Wu et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

“…Moreover, ISSR markers can be further converted into the more specific sequence-characterized amplified region (SCAR) markers (Aggarwal, Gupta, Banerjee, & Singh, 2011;Gao, Chen, & Liu, 2011;Wu et al, 2016). The species-specific SCAR markers have been successfully developed for identifying phytopathogenic fungi, such as Bipolaris sorokiniana (Aggarwal et al, 2011), Tilletia controversa (Gao et al, 2011) and Fusarium mangiferae (Wu et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Development of a sequence‐characterized amplified region marker using inter‐simple sequence repeats for detection of Puccinia striiformis f. sp. tritici

Wang

Chu

Liu

et al. 2017

Journal of Phytopathology

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Stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is the most devastating wheat disease in China. Early and accurate detection of the pathogens would facilitate effective control of the diseases. DNA‐based methods now provide essential tools for accurate plant disease diagnosis. In this study, inter‐simple sequence repeats (ISSR) technique has been successfully applied to develop a sequence‐characterized amplified region (SCAR) marker for diagnosis of stripe rust of wheat and detection of Pst. In this study, one fragment unique to Pst was identified by ISSR and then sequenced. Based on the specific fragment, a pair of SCAR primers (616AF/616AR) was designed to amplify a 299‐bp DNA fragment within the sequenced region. The primers can amplify a unique DNA fragment for all tested isolates of Pst but not for the other pathogens of wheat leaves and the uninfected leaves. The polymerase chain reaction (PCR) assay could detect as low as 0.1 ng of genomic DNA in a 25.0 μl PCR reaction mixture and detect the pathogen from asymptomatic wheat leaves inoculated with Pst under glasshouse conditions.

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Development of a sensitive molecular detection assay for mango malformation disease caused by Fusarium mangiferae

Abstract: Our assay provides a practical method for the early diagnosis so that proper prevention of the mango malformation disease can be developed.

Cited by 6 publications

References 10 publications

Transcription Profiling Analysis of Mango–Fusarium mangiferae Interaction

Transcription Profiling Analysis of Mango–Fusarium mangiferae Interaction

Cyanide produced with ethylene by ACS and its incomplete detoxification by β-CAS in mango inflorescence leads to malformation

Development of a sequence‐characterized amplified region marker using inter‐simple sequence repeats for detection of Puccinia striiformis f. sp. tritici

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