Development of a Sensitive Escherichia coli Bioreporter Without Antibiotic Markers for Detecting Bioavailable Copper in Water Environments

Pang, Yilin; Ren, Xiaojun; Li, Jianghui; Liang, Feng; Rao, Xiaoyu; Gao, Yang; Wu, Weibing; Li, Dong; Wang, Juanjuan; Zhao, Jianguo; Hong, Xufen; Jiang, Fengying; Wu, Wei; Zhou, Huaibin; Lyu, Jianxin; Tan, Guoqiang

doi:10.3389/fmicb.2019.03031

Cited by 7 publications

(5 citation statements)

References 73 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…When exposed to ultraviolet (UV) light, GFP fluoresces green. Pang et al replaced the copA gene with the GFP gene in E. coli and detected CuSO 4 concentrations between 0.39 and 78.68 μM [ 59 ]. Fluorescence was measured using a fluorescence spectrometer with linear readings ranging between 50 and 500 absolute fluorescence units (AFU).…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Potential Whole-Cell Biosensors for Detection of Metal Using MerR Family Proteins from Enterobacter sp. YSU and Stenotrophomonas maltophilia OR02

et al. 2021

View full text Add to dashboard Cite

Cell-based biosensors harness a cell’s ability to respond to the environment by repurposing its sensing mechanisms. MerR family proteins are activator/repressor switches that regulate the expression of bacterial metal resistance genes and have been used in metal biosensors. Upon metal binding, a conformational change switches gene expression from off to on. The genomes of the multimetal resistant bacterial strains, Stenotrophomonas maltophilia Oak Ridge strain 02 (S. maltophilia 02) and Enterobacter sp. YSU, were recently sequenced. Sequence analysis and gene cloning identified three mercury resistance operons and three MerR switches in these strains. Transposon mutagenesis and sequence analysis identified Enterobacter sp. YSU zinc and copper resistance operons, which appear to be regulated by the protein switches, ZntR and CueR, respectively. Sequence analysis and reverse transcriptase polymerase chain reaction (RT-PCR) showed that a CueR switch appears to activate a S. maltophilia 02 copper transport gene in the presence of CuSO4 and HAuCl4·3H2O. In previous studies, genetic engineering replaced metal resistance genes with the reporter genes for β-galactosidase, luciferase or the green fluorescence protein (GFP). These produce a color change of a reagent, produce light, or fluoresce in the presence of ultraviolet (UV) light, respectively. Coupling these discovered operons with reporter genes has the potential to create whole-cell biosensors for HgCl2, ZnCl2, CuSO4 and HAuCl4·3H2O.

show abstract

Section: Resultsmentioning

confidence: 99%

“…The MerR family switch then requires more metal to activate the reporter gene, decreasing the sensitivity of the biosensor. Pang et al removed the copA gene from E. coli to improve the sensitivity of copper detection [ 59 ]. All MerR family metal biosensors require deletion of the original resistance genes, but keeping their promoters.…”

Section: Resultsmentioning

confidence: 99%

Potential Whole-Cell Biosensors for Detection of Metal Using MerR Family Proteins from Enterobacter sp. YSU and Stenotrophomonas maltophilia OR02

et al. 2021

View full text Add to dashboard Cite

show abstract

“…The assessment of the heavy metal bioavailability is a hot topic in the research of WCBs. Table 2 summarizes the studies that applied WCB technology to detect various heavy metals such as (van Genuchten et al, 2018), Cr (Kim et al, 2020), Cd (Hui et al, 2021), copper (Cu) (Pang et al, 2020), mercury (Hg) (He et al, 2018), antimony (Sb) (Kim et al, 2020), Pb , silver (Ag) (Hurtado-Gallego et al, 2019a), iron (Fe) Frontiers in Chemistry frontiersin.org (Blanco-Ameijeiras et al, 2019), titanium (Ti) (Hurtado-Gallego et al, 2019a), zinc (Zn) (Kim et al, 2020), cobalt (Co.) (Martin-Betancor et al, 2017), and nickel (Ni) (Cayron et al, 2017). Different WCBs may have different sensitivities for the same metal.…”

Section: Heavy Metalsmentioning

confidence: 99%

Use of whole-cell bioreporters to assess bioavailability of contaminants in aquatic systems

et al. 2022

View full text Add to dashboard Cite

Water contamination has become increasingly a critical global environmental issue that threatens human and ecosystems’ health. Monitoring and risk assessment of toxic pollutants in water bodies is essential to identifying water pollution treatment needs. Compared with the traditional monitoring approaches, environmental biosensing via whole-cell bioreporters (WCBs) has exhibited excellent capabilities for detecting bioavailability of multiple pollutants by providing a fast, simple, versatile and economical way for environmental risk assessment. The performance of WCBs is determined by its elements of construction, such as host strain, regulatory and reporter genes, as well as experimental conditions. Previously, numerous studies have focused on the design and construction of WCB rather than improving the detection process and commercialization of this technology. For investigators working in the environmental field, WCB can be used to detect pollutants is more important than how they are constructed. This work provides a review of the development of WCBs and a brief introduction to genetic construction strategies and aims to summarize key studies on the application of WCB technology in detection of water contaminants, including organic pollutants and heavy metals. In addition, the current status of commercialization of WCBs is highlighted.

show abstract

“…Most of the current whole-cell copper biosensors were constructed in prokaryotic organisms (Bereza-Malcolm et al, 2015), e.g. Escherichia coli (Martinez et al, 2019;Pang et al, 2020), Pseudomonas sp. (Li et al, 2014;Maderova et .…”

Section: Introductionmentioning

confidence: 99%

Re-engineering of CUP1 promoter and Cup2/Ace1 transactivator to convert Saccharomyces cerevisiae into a whole-cell eukaryotic biosensor capable of detecting 10 nM of bioavailable copper

Žunar

Mosrin

Bénédetti

et al. 2022

Biosensors and Bioelectronics

View full text Add to dashboard Cite

Development of a Sensitive Escherichia coli Bioreporter Without Antibiotic Markers for Detecting Bioavailable Copper in Water Environments

Cited by 7 publications

References 73 publications

Potential Whole-Cell Biosensors for Detection of Metal Using MerR Family Proteins from Enterobacter sp. YSU and Stenotrophomonas maltophilia OR02

Potential Whole-Cell Biosensors for Detection of Metal Using MerR Family Proteins from Enterobacter sp. YSU and Stenotrophomonas maltophilia OR02

Use of whole-cell bioreporters to assess bioavailability of contaminants in aquatic systems

Re-engineering of CUP1 promoter and Cup2/Ace1 transactivator to convert Saccharomyces cerevisiae into a whole-cell eukaryotic biosensor capable of detecting 10 nM of bioavailable copper

Contact Info

Product

Resources

About