Development of a sensitive enzymeimmunoassay for oxytocin determination in bovine plasma

Prakash, B.S.; Metten, Maria; Schams, D.; Wuttke, W.

doi:10.1016/s0378-4320(98)00069-4

Cited by 18 publications

(13 citation statements)

References 11 publications

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“…[2][3][4][5] EIA gives good correlation with the published RIA techniques, validating the methodology used in this study and has the additional advantage of using non-radioactive material. 6 Peptides such as oxytocin have low molecular stability at room temperature and short half-lives so samples must be frozen to avoid degradation by aminopeptidases. Serum albumin can also affect measurements by binding to components of the EIA.…”

Section: Discussionmentioning

confidence: 99%

Serum oxytocin concentrations in elective caesarean delivery: a randomized comparison of three infusion regimens

Yamaguchi¹,

Cardoso²,

Torres³

et al. 2011

International Journal of Obstetric Anesthesia

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Section: Discussionmentioning

confidence: 99%

Serum oxytocin concentrations in elective caesarean delivery: a randomized comparison of three infusion regimens

Yamaguchi¹,

Cardoso²,

Torres³

et al. 2011

International Journal of Obstetric Anesthesia

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“…In 1994, the first ELISA suitable for measuring oxytocin in plasma was developed by Prakash et al . . They recognised that plasma components interfered by reducing antibody binding, and so they constructed standard curves in hormone‐stripped plasma.…”

Section: Clearance Of Oxytocinmentioning

confidence: 99%

Measuring Oxytocin and Vasopressin: Bioassays, Immunoassays and Random Numbers

Leng

Sabatier

2016

J Neuroendocrinology

186

148

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In this review, we consider the ways in which vasopressin and oxytocin have been measured since their first discovery. Two different ways of measuring oxytocin in widespread use currently give values in human plasma that differ by two orders of magnitude, and the values measured by these two methods in the same samples show no correlation. The notion that we should accept this seems absurd. Either one (or both) methods is not measuring oxytocin, or, by 'oxytocin', the scientists that use these different methods mean something very different. If these communities are to talk to each other, it is important to validate one method and invalidate the other, or else to establish exactly what each community understands by 'oxytocin'. A similar issue concerns vasopressin: again, different ways of measuring vasopressin give values in human plasma that differ by two orders of magnitude, and it appears that the same explanation for discrepant oxytocin measurements applies to discrepant vasopressin measurements. The first assays for oxytocin and vasopressin measured biological activity directly. When immunoassays were introduced, they encountered problems: high molecular weight factors in raw plasma interfered with the binding of antibodies to the hormones, leading to high and erroneous readings. When these interfering factors were removed by extraction of plasma samples, immunoassays gave measurements consistent with bioassays, with measures of turnover and with the sensitivity of target tissues to exogenous hormone. However, many recent papers use an enzyme-linked immunoassay to measure plasma levels without extracting the samples. Like the first radioimmunassays of unextracted plasma, this generates impossibly high and wholly erroneous measurements.

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“…Hence, it is significant to develop sensitive methods for the determination of OXT for food safety. In recent years, several techniques, such as high performance liquid chromatography (HPLC), liquid chromatography-mass spectrometry (LC-MS) [1], capillary electrophoresis [5], enzyme immunoassay procedures [6], have been reported for the determination of OXT. But, these techniques can require great amount of reagents and solvents.…”

Section: Introductionmentioning

confidence: 99%

Oxytocin imprinted polymer based surface plasmon resonance sensor and its application to milk sample

Yola

Atar

Erdem

2015

Sensors and Actuators B: Chemical

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Development of a sensitive enzymeimmunoassay for oxytocin determination in bovine plasma

Cited by 18 publications

References 11 publications

Serum oxytocin concentrations in elective caesarean delivery: a randomized comparison of three infusion regimens

Serum oxytocin concentrations in elective caesarean delivery: a randomized comparison of three infusion regimens

Measuring Oxytocin and Vasopressin: Bioassays, Immunoassays and Random Numbers

Oxytocin imprinted polymer based surface plasmon resonance sensor and its application to milk sample

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