Development of a sensitive enzyme immunoassay for measuring taipan venom in serum

Factor deficiencies in venom‐induced consumption coagulopathy resulting from Australian elapid envenomation: Australian Snakebite Project (ASP‐10)

“…A 72-year-old woman with complete VICC had an intracranial hemorrhage resulting in a dense right hemiparesis and neglect, which did not resolve. There were 681 blood samples in total with a median of four samples per patient admission (range: [1][2][3][4][5][6][7][8][9][10][11][12][13][14]. A summary of the lowest/highest values recorded for coagulation tests and factor levels for patients with complete VICC, partial VICC or no evidence of envenomation is presented in Table 1.…”

Detection of venom after antivenom administration is largely due to bound venom

“…Venom specific ELISA A venom specific ELISA was used to measure Russell's viper and brown snake venom as previously described (Kulawickrama et al, 2010). Microplates were coated with anti-D. russelii venom IgG antibodies (100 mL/well of 1 mg/ml in carbonate buffer) for 1 h at room temperature and overnight at 4 C. The plates were washed and blocking solution (300 mL/well) applied for one hour.…”

232. Death Adder Envenoming Causes Neurotoxicity not Reversed by Antivenom - Australian Snakebite Project (ASP-16)

“…Specific venom enzyme immunoassay was performed on all patient samples using a previously described technique developed for Australian snakebites [29]. All washings were done with 0.02% Tween 20 in PBS.…”

“…Previous studies of taipan (Oxyuranus sp.) venom have shown that using this approach the level of detection can be set as low as 0.15 ng/mL [29]. Twenty four non-envenomed serum samples were measured unspiked (blank) and spiked at a concentration such that the overlap of absorbance with the unspiked samples was #5%.…”