2010
DOI: 10.1016/j.toxicon.2010.03.003
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Development of a sensitive enzyme immunoassay for measuring taipan venom in serum

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Cited by 82 publications
(69 citation statements)
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“…A 72-year-old woman with complete VICC had an intracranial hemorrhage resulting in a dense right hemiparesis and neglect, which did not resolve. There were 681 blood samples in total with a median of four samples per patient admission (range: [1][2][3][4][5][6][7][8][9][10][11][12][13][14]. A summary of the lowest/highest values recorded for coagulation tests and factor levels for patients with complete VICC, partial VICC or no evidence of envenomation is presented in Table 1.…”
Section: Resultsmentioning
confidence: 99%
“…A 72-year-old woman with complete VICC had an intracranial hemorrhage resulting in a dense right hemiparesis and neglect, which did not resolve. There were 681 blood samples in total with a median of four samples per patient admission (range: [1][2][3][4][5][6][7][8][9][10][11][12][13][14]. A summary of the lowest/highest values recorded for coagulation tests and factor levels for patients with complete VICC, partial VICC or no evidence of envenomation is presented in Table 1.…”
Section: Resultsmentioning
confidence: 99%
“…Venom specific ELISA A venom specific ELISA was used to measure Russell's viper and brown snake venom as previously described (Kulawickrama et al, 2010). Microplates were coated with anti-D. russelii venom IgG antibodies (100 mL/well of 1 mg/ml in carbonate buffer) for 1 h at room temperature and overnight at 4 C. The plates were washed and blocking solution (300 mL/well) applied for one hour.…”
Section: Enzyme-linked Immunosorbent Assays (Elisa)mentioning
confidence: 99%
“…Specific venom enzyme immunoassay was performed on all patient samples using a previously described technique developed for Australian snakebites [29]. All washings were done with 0.02% Tween 20 in PBS.…”
Section: Enzyme Immunoassaymentioning
confidence: 99%
“…Previous studies of taipan (Oxyuranus sp.) venom have shown that using this approach the level of detection can be set as low as 0.15 ng/mL [29]. Twenty four non-envenomed serum samples were measured unspiked (blank) and spiked at a concentration such that the overlap of absorbance with the unspiked samples was #5%.…”
Section: Level Of Detection/level Of Blank Determinationmentioning
confidence: 99%