Development of a sensitive and fast method for detection of catecholamines and metabolites by HRMS

Roiffé, Rafaela Rocha; Ribeiro, William; Sardela, Vinícius Figueiredo; Cruz, Márcia N. S. de la; Souza, Kátia R. de; Pereira, Henrique Marcelo Gualberto; Neto, Francisco Radler de Aquino

doi:10.1016/j.microc.2019.104173

Cited by 9 publications

(4 citation statements)

References 22 publications

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“…A capillary temperature, that helps the analytes to be emitted in solution form, was also tested and varied between 260, 280, 300, 320, 340, 360, and 380 • C. Capillary temperatures between 280-320 • C provided similar and satisfactory results with some differentiations for more acidic compounds which exhibited higher sensitivity in high capillary temperatures. This observation is in accordance with another published work [59]. There is no ideal capillary temperature that fits equally for all the analytes of interest, so 320 • C was selected as optimal.…”

Section: Mass Spectrometry-ltq Orbitrap Esi Conditionssupporting

confidence: 88%

Exploring the Efficiency of UHPLC-Orbitrap MS for the Determination of 20 Pharmaceuticals and Acesulfame K in Hospital and Urban Wastewaters with the Aid of FPSE

Kalaboka¹,

Chrimatopoulos²,

Jiménez‐Holgado³

et al. 2020

Separations

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Aside from the classical residues of persistent organic pollutants (POPs), the occurrence of emerging contaminants (ECs) in the environment has become a subject of increasing concern due to their harmful impact on the aquatic environment. Wastewater treatment plant (WWTP) effluents are major sources of environmental pollution. Therefore, data concerning their existence is required. In this study, twenty compounds representative of different drug groups considered ECs and belonging to antibiotics, antipsychotics, anti-inflammatory drugs plus acesulfame K were selected to be accurately detected and quantified with UHPLC–LTQ-Orbitrap MS in hospital and urban WWTP effluents. Chromatographic parameters (column efficiency, mobile phase, etc.), as well as mass spectrometry conditions concerning ionization mode and Orbitrap analysis (ESI options, mass resolving power, AGC target, tube lens, injection time), were evaluated. Moreover, a novel fabric phase sorptive extraction (FPSE) method based on fiber glass coated with PEG300 was employed as sample preparation process. Experimental parameters affecting extraction and desorption steps such as sample pH, extraction time, ionic strength, elution time and solvent have been optimized. The optimized methodology was validated providing excellent linearity (R2 > 0.99), and low detection and quantification limits up to 3.1 and 9.3 ng/L, for carbamazepine, respectively. Relative recoveries ranged from 81.1% to 114.0%, while a medium matrix effect for most of the target compounds occurred. Applying the above analytical method in effluents of WWTPs from NW Greece, nine compounds were quantified with concentrations that varied from 55.4 to 728.4 ng/L.

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Section: Mass Spectrometry-ltq Orbitrap Esi Conditionssupporting

confidence: 88%

Exploring the Efficiency of UHPLC-Orbitrap MS for the Determination of 20 Pharmaceuticals and Acesulfame K in Hospital and Urban Wastewaters with the Aid of FPSE

Kalaboka¹,

Chrimatopoulos²,

Jiménez‐Holgado³

et al. 2020

Separations

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show abstract

“…Still, Khamis et al [6] assessed linearity, precision, accuracy, dilution integrity, selectivity, carryover, and stability to validate a method for the absolute quantification of amino/ phonel metabolites. Therefore, many works are related to the biological, pharmaceutical and chemical areas [4][5][7][8][9][10][11][12]. However, we have not found a method validation for fatigue tests in kingpins, as proposed in the current study.…”

Section: Introductionmentioning

confidence: 79%

“…Several approaches may be used to validate test methods. Roiffé et al [4] analysed repeatability, intermediate accuracy, matrix interference, limit of quantification, linearity, carryover and robustness to validate new analytical method for the analysis of catecholamines and its biotransformation products.…”

Section: Introductionmentioning

confidence: 99%

Method validation for fatigue testing in kingpins

Buzzatti¹,

Fabrício²,

Lemos³

et al. 2021

Tecnol. Metal. Mater. Min.

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“…The instrument parameters were set as follows: Spray voltages were 3500 V and the capillary and probe heater temperatures were set to 320 and 350°C, respectively. Sheath gas and aux gas flow rate were 50 and 10 (in arbitrary units), respectively, and the S-lens RF level was 60 ( Roiffé et al, 2019 ).…”

Section: Methodsmentioning

confidence: 99%

Identification of Veratrum Species in Pimacao Based on ITS2 Sequences and Steroidal Alkaloids by a Pseudo-Targeted Metabolomics Method

Wang

Yin

et al. 2022

Front. Plant Sci.

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Pimacao is a traditional Chinese folk medicine and is the main component of the famous Chinese herbal remedy “Yunnan Baiyao” for its significant analgesic activity in the treatment of wounds. Due to increases in consumption, its wild population is now difficult to find, and adulterant from the same genus has occurred. However, this is challenging to distinguish the species of Veratrum in Pimacao using dried roots and rhizomes or medicinal powder. ITS2 sequences and steroidal alkaloids by the non-targeted and pseudo-targeted metabolomics methods were taken advantage of establishing an effective identification method. Based on the ITS2 sequence, metabolite profiling of steroidal alkaloids and morphological characteristics, the classification of two distinct subspecies in V. mengzeanum has been reinforced. In addition, the new subspecies V. mengzeanum subsp. phuwae was collected in China for the first time. The ITS2 sequence could be used in the identification of V. taliense, V. mengtzeanum, V. stenophyllum, and V. nigrum, but is insufficient for intraspecific identification. Simultaneously, 147 variables were labeled by non-targeted analysis accomplished utilizing an ultra-high-performance liquid chromatography electrospray ionization orbitrap tandem mass spectrometry (UPLC-ESI-QE-Orbitrap-MS) system consisting of an Orbitrap QE HF-X. Followed by a pseudo-targeted analysis method developed for the Qtrap 6500-plus mass spectrometry system coupled with an ESI source, 29 labeled steroidal alkaloids detected by the MRM mode could distinguish between four species. Notably, 25 labeled steroidal alkaloids could distinguish between three closely related species. These have the potential to be used as markers for identification. Furthermore, there were several variables with statistical differences between two subspecies of V. mengtzeanum and populations of V. taliense, V. mengtzeanum, and V. stenophyllum.

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Development of a sensitive and fast method for detection of catecholamines and metabolites by HRMS

Cited by 9 publications

References 22 publications

Exploring the Efficiency of UHPLC-Orbitrap MS for the Determination of 20 Pharmaceuticals and Acesulfame K in Hospital and Urban Wastewaters with the Aid of FPSE

Exploring the Efficiency of UHPLC-Orbitrap MS for the Determination of 20 Pharmaceuticals and Acesulfame K in Hospital and Urban Wastewaters with the Aid of FPSE

Method validation for fatigue testing in kingpins

Identification of Veratrum Species in Pimacao Based on ITS2 Sequences and Steroidal Alkaloids by a Pseudo-Targeted Metabolomics Method

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