Development of a semi-quantitative real-time RT-PCR for the detection of measles virus

Mubarak, Hassan; Swart, Rik L. de; Osterhaus, Albert D. M. E.; Schutten, Martin

doi:10.1016/j.jcv.2004.08.017

Cited by 33 publications

(19 citation statements)

References 21 publications

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“…These results are in agreement with many other previous studies El Mubarak et al 18 who documented that qRT-PCR is efficient rapid technique for detecting measles infection or vaccination. Also, Ammour et al, 19 reported that qRT-PCR was successfully used for estimating the titers of measles, A B…”

Section: Discussionsupporting

confidence: 93%

Correlation between live attenuated measles viral load and growth inhibition percentage in non-small cell lung cancer cell line

Obaid

Obied

Al-Jibouri

et al. 2018

J. contemp. med. sci.

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, 10 −5 of virus stock (8.860 log 10 RNA copies/µl). The virus cytopathic effect (CPE) was studied at 24, 48, 72, and 96 hr. Oncolytic effects of the virus were assessed by cytotoxicity assay, apoptosis analysis, and proliferation marker Ki-67. The growth inhibition percentage was determined by Enzyme-Linked Immunosorbent Assay (ELISA) reader using crystal violet staining assay and compared with the viral load measured by quantitative real time-PCR. Results The results of the study showed that measles virus had an inhibitory effect on the growth of human lung cancer cell line compared with untreated control group. There was highly significant positive correlation between viral load and PGI at 24, 48, 72, 96 hr (P < 0.001). The growth inhibition rate of a cell was associated with a decrement in the expression of the Ki-67 protein and increase in the ratio of early and late apoptotic cells in comparison with untreated control group. Conclusion Live attenuated measles virus strain induced cytotoxic effect against human lung cancer cell line (A549) by induction of apoptosis as an important mechanism of anti-tumor activity, in addition, it indicates a correlation between the quantity of MV genomes and percentage of growth inhibition. This relation has proved that measles virus had anticancer effect.

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Section: Discussionsupporting

confidence: 93%

Correlation between live attenuated measles viral load and growth inhibition percentage in non-small cell lung cancer cell line

Obaid

Obied

Al-Jibouri

et al. 2018

J. contemp. med. sci.

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“…infusion of 10 ml of phosphate-buffered saline (PBS) through a flexible catheter. Bronchoalveolar lavage (BAL) cells were resuspended in culture medium with supplements as described above and used directly for virus isolation or virus detection by real-time RT-PCR (18). Throat and nose swabs were collected 0, 3, 6, and 9 d.p.i.…”

Section: Methodsmentioning

confidence: 99%

“…Virus isolations were monitored for cytopathic effect and/or fluorescence on day 6 after isolation. Real-time RT-PCR for BAL samples, and throat and nose swabs were performed as described previously (18). The results are expressed as "ϩ", indicating virus/genome was isolated/detected (regardless of load), or "-", indicating no virus was isolated/detected.…”

Section: Methodsmentioning

confidence: 99%

Live-Attenuated Measles Virus Vaccine Targets Dendritic Cells and Macrophages in Muscle of Nonhuman Primates

Rennick

Vries

Carsillo

et al. 2015

J Virol

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Although live-attenuated measles virus (MV) vaccines have been used successfully for over 50 years, the target cells that sustain virus replication in vivo are still unknown. We generated a reverse genetics system for the live-attenuated MV vaccine strain Edmonston-Zagreb ( IMPORTANCE Even though MV strain Edmonston-Zagreb has long been used as a live-attenuated vaccine (LAV) to protect against measles, nothing is known about the primary cells in which the virus replicates in vivo.This is vital information given the push to move toward needle-free routes of vaccination, since vaccine virus replication is essential for vaccination efficacy. We have generated a number of recombinant MV strains expressing enhanced green fluorescent protein. The virus that best mimicked the nonrecombinant vaccine virus was formulated according to protocols for production of commercial vaccine virus batches, and was subsequently used to assess viral tropism in nonhuman primates. The virus primarily replicated in professional antigen-presenting cells, which may explain why this LAV is so immunogenic and efficacious.

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“…From 25 March clinical specimens (throat swabs, samples of serum, oral fluid and urine) from confirmed and possible outbreak cases were sent to the Austrian National Reference Centre for Measles by local physicians, hospitals and local laboratories for serological confirmation of measles infection, for measles virus detection and genotyping. Measles virus RNA in clinical specimens was detected as described by El Mubarak et al [9] and genotyping was performed as described by Santibanez et al [10].…”

Section: Laboratory Investigationmentioning

confidence: 99%

Measles outbreak linked to a minority group in Austria, 2008

et al. 2009

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SUMMARYWe report on a measles outbreak originating in an anthroposophic community in Austria, 2008. A total of 394 (94 . 9 %) cases fulfilled the outbreak case definition including 168 cases affiliated to the anthroposophic community. The source case was a school pupil from Switzerland. The Austrian outbreak strain was genotype D5, indistinguishable from the Swiss outbreak strain. A school-based retrospective cohort study in the anthroposophic school demonstrated a vaccine effectiveness of 97 . 3 % in pupils who had received a single dose of measles-containing vaccine and 100 % in those who had received two doses. The vaccination coverage of the cases in the anthroposophic community was 0 . 6 %. Of the 226 outbreak cases not belonging to the anthroposophic community, the 10-24 years age group was the most affected. Our findings underline the epidemiological significance of suboptimal vaccination coverage in anthroposophic communities and in older age groups of the general population in facilitating measles virus circulation. The findings of this outbreak investigation suggest that the WHO European Region is unlikely to achieve its 2010 target for measles and rubella elimination.

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Development of a semi-quantitative real-time RT-PCR for the detection of measles virus

Cited by 33 publications

References 21 publications

Correlation between live attenuated measles viral load and growth inhibition percentage in non-small cell lung cancer cell line

Correlation between live attenuated measles viral load and growth inhibition percentage in non-small cell lung cancer cell line

Live-Attenuated Measles Virus Vaccine Targets Dendritic Cells and Macrophages in Muscle of Nonhuman Primates

Measles outbreak linked to a minority group in Austria, 2008

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