Development of a self-driving bioassay based on diffusion for simple detection of microorganisms

Wang, Jhih-Cheng; Chi, Shao-Wen; Shieh, Dar‐Bin; Chuang, Huey-Ru

doi:10.1016/j.snb.2018.09.087

Cited by 9 publications

(10 citation statements)

References 23 publications

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“…On the basis of our previous achievements in diffusometric techniques ( Cheng and Chuang, 2019 ; Chuang et al, 2018 ; Wang et al, 2018 , 2019 , 2020 ; Yang et al, 2020 ), we propose a rapid, specific, and sensitive detection of SARS-CoV-2 cDNA fragment in the recombinant plasmid as a proof of concept for COVID-19 diagnosis ( Fig. 1 ).…”

Section: Introductionmentioning

confidence: 99%

Rotational diffusometric sensor with isothermal amplification for ultra-sensitive and rapid detection of SARS-CoV-2 nsp2 cDNA

Das

Lin

Kwon

et al. 2022

Biosensors and Bioelectronics

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Section: Introductionmentioning

confidence: 99%

Rotational diffusometric sensor with isothermal amplification for ultra-sensitive and rapid detection of SARS-CoV-2 nsp2 cDNA

Das

Lin

Kwon

et al. 2022

Biosensors and Bioelectronics

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“…[24][25][26] Due to its ease of use, robustness, and ability to mitigate the use of complex thermal cycling processes, LAMP is currently being implemented in rapid diagnostics. [27] Combining the promising advantages of LAMP and our previous achievements on diffusometric method, [28][29][30][31][32][33] we wish to develop a novel biosensor to carry out a rapid, simple, and quantitative detection of pathogens using Janus particles to quantify the rotational Brownian motion, using E. coli as a proof of concept (Figure 1a). According to the Figure 1.…”

Section: Introductionmentioning

confidence: 99%

Ultrafast and Sensitive Screening of Pathogens by Functionalized Janus Microbeads‐Enabled Rotational Diffusometry in Combination with Isothermal Amplification

et al. 2022

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Polymerase chain reaction (PCR) is widely considered the gold standard in molecular diagnostics because of its high accuracy. However, conventional PCR is very time‐consuming (turnaround time 4–6 h), labor‐intensive, and high‐tech instruments dependent. Therefore, developing an ultrafast and sensitive nucleic amplification‐based detection method is still in high demand. Herein, a rapid screening of Escherichia coli using an advanced and sensitive biosensing method known as rotational diffusometry in combination with loop‐mediated isothermal amplification (LAMP) is reported. Given that the solution viscosity is increased with nucleic acid amplification, the viscosity change can be measured by the functionalized Janus microbeads‐enabled rotational diffusometry. The rotational diffusivity derived from capturing images of microbeads is expressed in terms of blinking signals. Under such conditions, this method can achieve a limit of detection of 42.8 fg μL−1 in 10 min for E. coli with sample volume as low as 2 μL. Additionally, detection of E. coli whole cells extracted from artificially contaminated milk, juice, and water is performed by the developed method and validated with real‐time PCR. Through this biosensing technique, a rapid, reliable, and low sample volume screening tool can be established, improving the shortcomings of the current time‐consuming and labor‐intensive methods.

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“…Brownian motion can be performed in two ways, namely, translational and rotational Brownian motion. The former is commonly investigated in a wide variety of applications [ 1 , 2 , 3 , 4 , 5 , 6 , 7 , 8 , 9 , 10 , 11 , 12 , 13 ], whereas the latter receives less attention because of its difficulty in realization. However, the rotational Brownian motion is inversely proportional to cubic particle diameter [ 14 ], which makes it highly sensitive to particle size change.…”

Section: Introductionmentioning

confidence: 99%

Development of a Self-Viscosity and Temperature-Compensated Technique for Highly Stable and Highly Sensitive Bead-Based Diffusometry

Chen

Chuang

2022

Biosensors

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Brownian motion, which is a natural phenomenon, has attracted numerous researchers and received extensive studies over the past decades. The effort contributes to the discovery of optical diffusometry, which is commonly used for micro/nano particle sizing. However, the analysis uncertainty caused by the coupling relationship among particle diameter, temperature, and fluid viscosity usually poses a barrier to precise measurement. Preventing random background noise becomes the key to achieving a high level of accuracy in diffusometry detection. Recently, Janus particles have become known as an ideal tool for resolving the rotational Brownian motion. Followed by our previous study, the rotational Brownian motion and the translational Brownian motion can be separately measured using the Janus particles. Accordingly, a simple self-viscosity and temperature-compensated technique based on the delicate removal of temperature and fluid viscosity variations through particle tracking was first proposed in this study. Consequently, the translational Brownian motion was expressed in terms of particle trajectory, whereas the rotational Brownian motion was expressed in terms of the blinking signal from the Janus particles. The algorithm was verified simulatively and experimentally in temperature (10 °C to 40 °C) and viscosity-controlled (1 mPa·s to 5 mPa·s) fields. In an evaluation of biosensing for a target protein, IFN-γ, the limit of detection of the proposed self-compensated diffusometry reached 0.45 pg/mL, whereas its uncertainties of viscosity and temperature were 96 and 15-fold lower than the pure the rotational Brownian motion counterpart, respectively. The results indicated the low-uncertainty and high-accuracy biosensing capability resulting from the self-viscosity and temperature-compensated technique. This research will provide a potential alternative to future similar bead-based immunosensing, which requires ultra-high stability and sensitivity.

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Development of a self-driving bioassay based on diffusion for simple detection of microorganisms

Cited by 9 publications

References 23 publications

Rotational diffusometric sensor with isothermal amplification for ultra-sensitive and rapid detection of SARS-CoV-2 nsp2 cDNA

Rotational diffusometric sensor with isothermal amplification for ultra-sensitive and rapid detection of SARS-CoV-2 nsp2 cDNA

Ultrafast and Sensitive Screening of Pathogens by Functionalized Janus Microbeads‐Enabled Rotational Diffusometry in Combination with Isothermal Amplification

Development of a Self-Viscosity and Temperature-Compensated Technique for Highly Stable and Highly Sensitive Bead-Based Diffusometry

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