Development of a screen-printed carbon electrochemical immunosensor for picomolar concentrations of estradiol in human serum extracts

Electrochemical detection of 17β-estradiol using DNA aptamer immobilized gold electrode chip

“…These assays can give the information about the concentration of endocrine disrupting chemicals (EDCs) including 17␤-estradiol and their activities inside the cell, but they normally require a long detection time with a low specificity due to a wide range of hEr affinities to EDCs. Recently, the immunoassays and nanobiosensors using nanoparticles have been developed as sensitive and rapid methods for the detection of 17␤-estradiol by utilizing antibodies or functional polymers (Glass et al, 2004;Miyashita et al, 2005;Pemberton et al, 2005;Lin and Li, 2006;Wei et al, 2006). The specificities of these biosensors were reported to be better than hER-based biosensors due to their structure-specific biorecognition.…”

A recombinant Fab fragment-based electrochemical immunosensor for the determination of testosterone in bovine urine

“…They are widely applied in the area of human and animal health and in the food sector (D'Orazio, 2003;Mello and Kubota, 2002). Electrochemical-based screen-printed electrodes (SPE) provide an approach to develop cost-effective devices through disposable means and have been reported to measure steroids, like progesterone in cow's milk (Pemberton et al, 1998;Kreuzer et al, 2004) and estradiol in human serum (Pemberton et al, 2005).…”

Use of whole blood for analysis of disease-associated biomarkers

“…Bio-sensing technology, for example an electrochemical approach such as that used by diabetics for glucose monitoring using WB [10,11] could offer a rapid, low cost alternative. An electrochemical method incorporating an antibody immobilised onto a screen-printed carbon electrode has been developed by our group for detection of picomolar concentrations of estradiol [12] and could be adapted for measurement of soluble markers in WB. There is potential to develop a multiplex system, enabling simultaneous detection of a panel of biomarkers.…”