Development of a robust microtiter plate-based assay method for assessment of bioactivity

Casey, John; O’Cleirigh, Cormac C.; Walsh, Padraig K.; O’Shea, Daniel

doi:10.1016/j.mimet.2004.04.017

Cited by 66 publications

(63 citation statements)

References 23 publications

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“…To determine whether the presence of the FA and SA had some effect on the bacterial growth in liquid medium, the minimum inhibitory concentration, or MIC (considered the lowest concentration of an antimicrobial that will inhibit the growth of a micro-organism after incubation) was determined using a microtiter plate-based assay method (Casey et al, 2004). Several concentrations of the phenolic acids were prepared by diluting the stock solutions in the appropriated DMSO.…”

Section: Growth-inhibitory Activity -Minimum Inhibitory Concentrationmentioning

confidence: 99%

The effects of ferulic and salicylic acids on Bacillus cereus and Pseudomonas fluorescens single- and dual-species biofilms

Lemos

Borges

Teodósio

et al. 2014

International Biodeterioration & Biodegradation

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Biofilms are a problem to food industries, causing equipment damage, increased energy costs, and food spoilage, and they are a potential harbour of pathogenic microorganisms. Their extreme anti-microbial resistance means that novel control strategies are necessary. Plant secondary metabolites (phyto-chemicals) have demonstrated promising antimicrobial properties when applied against planktonic cells and biofilms. The aim of this study was to test the effectiveness of two phenolic acids: ferulic (FA) and salicylic (SA), alone and in combination (FSA) on the prevention and control of Bacillus cereus and Pseudomonas fluorescens biofilms. Additional tests were performed to ascertain the effects of FA and SA on bacterial motility, surface properties (physicochemical properties and surface charge), and quorum sensing inhibition (QSI). The effects of a concentration two times the minimum inhibitory concentration (500 g mL -1 ) were assessed on single-and dual-species biofilms. The results demonstrated that only swimming was affected by FA and SA and no clear relationship was obtained between the effects of phenolic acids on motility and biofilm prevention. The bacterial physico-chemical surface properties and charge were affected by the phenolic acids. Salicylic acid demonstrated capacity for QSI. However, both bacteria were able to form single-and dual-species biofilms in the presence of the phenolic acids. The application of FA and SA (single and combined) to biofilms caused low to moderate inactivation and removal. However, dual-species biofilms formed in the presence of phenolic acids were highly susceptible to a second exposure to the chemicals. The continuous exposure of dual-species biofilms to the phenolic acids decreased their resilience and resistance to inactivation and removal. This study clarifies the role of FA and SA in the prevention and control of biofilms formed by two important food spoilage bacteria.2

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Section: Growth-inhibitory Activity -Minimum Inhibitory Concentrationmentioning

confidence: 99%

The effects of ferulic and salicylic acids on Bacillus cereus and Pseudomonas fluorescens single- and dual-species biofilms

Lemos

Borges

Teodósio

et al. 2014

International Biodeterioration & Biodegradation

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“…This assay, although good, produces predominantly qualitative results (Casey et al, 2004) and can lead to interpretational problems, including in-growth in the zone of inhibition (Piliouras et al, 2002) and subjectivity associated with visual assessments. This study uses the microtiter plate-based assay which is a robust and quantitative method free from mathematical inaccuracies.…”

Section: Resultsmentioning

confidence: 99%

“…The antibacterial activity of the solvent extracts was expressed in terms of percentage of inhibition and was calculated by the following formula (Casey et al, 2004):…”

Section: Methodsmentioning

confidence: 99%

Application of Baranyi function to model the antibacterial properties of solvent extract from Irish York cabbage against food spoilage and pathogenic bacteria

Jaiswal¹,

Gupta²,

Abu‐Ghannam³

et al. 2011

Food sci. technol. int.

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Cabbage is a rich source of a number of bioactive compounds such as flavonoids, glucosinolates and their breakdown products which may have antibacterial, antioxidant and anticancer properties. Outer green leaves of York cabbage were extracted with 60% methanol, ethanol or acetone. Antibacterial activities of vacuum dried crude extracts were estimated against a number of Gram-positive and Gram-negative food spoilage and food pathogenic bacteria. The crude extracts showed a broad spectrum of antibacterial activities but 60% methanol extract exhibited the highest antibacterial effect. Complete growth inhibition for Listeria monocytogenes was achieved with an extract concentration of 1.4%, whereas a two-fold concentration was required to achieve a reduction of 75% and 64% for Salmonella abony and Pseudomonas aeruginosa, respectively. For Enterococcus faecalis methanolic extract showed a weak inhibition only (31%). The lower concentrations of methanolic extract from York cabbage prolonged the lag phase and reduced both the exponential growth rate and final population densities of the culture. Survival of the micro-organisms in presence of methanolic extract was mathematically modeled using Baranyi model equations.

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“…Antibacterial activities of capped and uncapped AgNPs of different silver concentrations were determined by a microtitre well method [7]. Samples were doubly diluted in water (100 L) in microtitre wells and bacteria (100 L; 10 6 CFU/mL) M a n u s c r i p t 8 were added.…”

Section: Antibacterial Activity Assaymentioning

confidence: 99%

“…AgNP antibacterial activities were determined by calculating the percentage inhibition of growth using the formula [7]:…”

Section: Data Analysis 251 Percentage Inhibition Of Growthmentioning

confidence: 99%

Enhancement of the antibacterial properties of silver nanoparticles using β-cyclodextrin as a capping agent

Jaiswal

Duffy

Jaiswal³

et al. 2010

International Journal of Antimicrobial Agents

150

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Silver nanoparticles (AgNPs) were synthesised by reducing silver salts using NaBH 4 followed by capping with varying concentrations of -cyclodextrin (-CD) and were physically characterised. Antibacterial activity against Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus was determined by a microtitre well method.The AgNPs were spherical under transmission electron microscopy, whilst dynamic light scattering showed average diameters of capped particles to be smaller (4-7 nm) than their uncapped equivalents (17 nm). Capped particles demonstrated superior photostability when exposed to intense ultraviolet radiation for 4 h as well as significantly (P < 0.05) higher (up to 3.5-fold) antibacterial activity. The influence of -CD concentration was seen to delay bacterial growth, indicating that a Trojan horse mechanism may be occurring owing to bacterial carbohydrate affinity, thereby enhancing silver ion absorption.

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Development of a robust microtiter plate-based assay method for assessment of bioactivity

Cited by 66 publications

References 23 publications

The effects of ferulic and salicylic acids on Bacillus cereus and Pseudomonas fluorescens single- and dual-species biofilms

The effects of ferulic and salicylic acids on Bacillus cereus and Pseudomonas fluorescens single- and dual-species biofilms

Application of Baranyi function to model the antibacterial properties of solvent extract from Irish York cabbage against food spoilage and pathogenic bacteria

Enhancement of the antibacterial properties of silver nanoparticles using β-cyclodextrin as a capping agent

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