Development of a Ribosomal DNA ITS2 Marker for the Identification of the Thrips,<i>Scirtothrips dorsalis</i>

Farris, Roxanne E.; Ruiz‐Arce, Raul; Ciomperlik, Matthew A.; Vasquez, J. D.; DeLeon, Ricardo

doi:10.1673/031.010.2601

Cited by 32 publications

(26 citation statements)

References 27 publications

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“…Some of them are based on the internal transcribed spacer (ITS) region of the nuclear ribosomal DNA (rDNA) (Moritz et al 2002;Toda and Komazaki 2002;Rugman-Jones et al 2006;Farris et al 2010) and the other protocols are based on a portion of the mitochondrial cytochrome oxidase I (COI) coding gene (Brunner et al 2002;Asokan et al 2007). Both the ITS and COI regions show both high interspecific variability and extremely low intraspecific variability (Brunner et al 2002;Moritz et al 2002).…”

Section: Methods Based On Dna Analysismentioning

confidence: 99%

“…The mitochondrial COI gene has also been confirmed to be a suitable marker for thrips species identification by Frey and Frey (2004). While Asokan et al (2007) developed a species-specific PCR for T. tabaci and T. palmi and Farris et al (2010) did so for Scirtothrips dorsalis, most other PCR assays need further analysis, e.g., by RFLP or by sequencing PCR products.…”

Section: Methods Based On Dna Analysismentioning

confidence: 99%

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Traditional and modern methods for the identification of thrips (Thysanoptera) species

Mehle

Trdan

2012

J Pest Sci

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Many thrips are pests of commercial crops due to the damage they cause by feeding on developing flowers or vegetables. Thrips may also serve as vectors for plant diseases, such as tospoviruses. Their small size and predisposition towards enclosed places makes them difficult to detect by phytosanitary inspection. In this review, several methods available for identifying thrips, including their advantages and disadvantages, are discussed. A combination of different methods gives the most reliable identification. Relatively new morphometric, molecular and biochemical methods for identifying thrips species represent valuable alternatives for situations in which correct identification with classical morphological methods is very difficult, time consuming or virtually impossible. However, traditional morphological methods should not be neglected, especially because adequate identification using morphological keys is usually an indispensable first step in the development and validation of these new modern methods. In addition, modern systems may still require specimen identification to the genus level via morphological keys, or such keys may be recommended to confirm the results of modern identification methods.

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Section: Methods Based On Dna Analysismentioning

confidence: 99%

Section: Methods Based On Dna Analysismentioning

confidence: 99%

Traditional and modern methods for the identification of thrips (Thysanoptera) species

Mehle

Trdan

2012

J Pest Sci

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“…The method is useful at the species level. A molecular marker (rDNA ITS2) was developed by Farris et al (2010) for the speciesspecific identification of S. dorsalis specimens. A new identification method combining both morphological and molecular methods has been published by Kumar (2012) and can be applied to a single specimen.…”

Section: Detection and Identification Of The Pestmentioning

confidence: 99%

Scientific Opinion on the pest categorisation of Scirtothrips dorsalis

Rossi¹

2014

EFS2

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The EFSA Panel on Plant Health undertook a pest categorisation for the insect Scirtothrips dorsalis for the European Union. Although there are reports that this is a species complex and there are host-specific races, it is generally treated as a distinct taxon. Both morphological and molecular methods are required to confirm identification. Apart from one long-term outbreak in a botanic garden glasshouse in England, it is absent from the EU. Native to southern and eastern Asia, this species has been introduced to tropical and subtropical areas of Africa, Australasia and the Americas in recent years. It is highly polyphagous, with over 225 known hosts, which include many important EU crops. Southern areas of the EU are potentially suitable for outdoor establishment and it could establish in protected cultivation throughout the EU. Based on its phenology, the Panel showed that the climate in southern Europe could allow a similar number of generations to develop as in Japan and South Korea, where significant damage occurs to citrus and other crops outdoors. In protected cultivation, even though control methods used against other thrips species may be effective in keeping populations at low densities, damage can still occur at these densities owing to the transmission of tospoviruses. Despite being highly polyphagous, S. dorsalis is an insect listed in Annex IIAI of Council Directive 2000/29/EC only in relation to Citrus, Fortunella and Poncirus plants. These hosts are also regulated in Annex III and Annex V. They are also explicitly mentioned in Council Directive 2008/90/EC.

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“…The ribosomal DNA (rDNA) second internal transcribed spacer (ITS2) is another frequently used marker. This fast evolving nuclear region has proved its benefits for phylogenetic analysis within genera or for discrimination of cryptic species within complexes (e.g., [17,18]).…”

Section: Introductionmentioning

confidence: 99%

Molecular Test to Assign Individuals within the Cacopsylla pruni Complex

2013

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Crop protection requires the accurate identification of disease vectors, a task that can be made difficult when these vectors encompass cryptic species. Here we developed a rapid molecular diagnostic test to identify individuals of Cacopsylla pruni (Scopoli, 1763) (Hemiptera: Psyllidae), the main vector of the European stone fruit yellows phytoplasma. This psyllid encompasses two highly divergent genetic groups that are morphologically similar and that are characterized by genotyping several microsatellite markers, a costly and time-consuming protocol. With the aim of developing species-specific PCR primers, we sequenced the Internal Transcribed Spacer 2 (ITS2) on a collection of C . pruni samples from France and other European countries. ITS2 sequences showed that the two genetic groups represent two highly divergent clades. This enabled us to develop specific primers for the assignment of individuals to either genetic group in a single PCR, based on ITS2 amplicon size. All previously assigned individuals yielded bands of expected sizes, and the PCR proved efficient on a larger sample of 799 individuals. Because none appeared heterozygous at the ITS2 locus (i.e., none produced two bands), we inferred that the genetic groups of C . pruni , whose distribution is partly sympatric, constitute biological species that have not exchanged genes for an extended period of time. Other psyllid species (Cacopsylla, Psylla, Triozidae and Aphalaridae) failed to yield any amplicon. These primers are therefore unlikely to produce false positives and allow rapid assignment of C . pruni individuals to either cryptic species.

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Development of a Ribosomal DNA ITS2 Marker for the Identification of the Thrips,Scirtothrips dorsalis

Cited by 32 publications

References 27 publications

Traditional and modern methods for the identification of thrips (Thysanoptera) species

Traditional and modern methods for the identification of thrips (Thysanoptera) species

Scientific Opinion on the pest categorisation of Scirtothrips dorsalis

Molecular Test to Assign Individuals within the Cacopsylla pruni Complex

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