Development of a reversible binding process for in situ removal of 3-hydroxypropionaldehyde during biotechnological conversion of glycerol

Biotransformation of glycerol to 3-hydroxypropionaldehyde: Improved production by in situ complexation with bisulfite in a fed-batch mode and separation on anion exchanger

“…Complexation of 3HPA with sodium bisulfite and with bisulfite-functionalized anion exchange resin, respectively, has been reported earlier by Rütti et al (2011). The bound 3HPA could be eluted from the resin using saturated NaCl solution.…”

“…However, liberation of free 3HPA from the complex is problematic requiring harsh conditions (unpublished data). On the other hand, the complex of 3HPA with bisulfite is less strong and can be easily dissociated (Rütti et al, 2011).…”

“…Accumulation of 3HPA (above 30.00 mM) results in inhibition of biocatalytic activity of the cells and loss of cell viability through interaction with amino-and thiol-groups in proteins and other molecules in the cells (Chen et al, 2002;Cleusix et al, 2007;Schaefer et al, 2010;Vollenweider et al, 2010). Different techniques have been investigated for enhancing 3HPA production and protection of the producer microorganism such as engineering of glycerol dehydratase (Gibson et al, 2011), knockout of the genes encoding enzymes catalyzing the reduction of 3HPA to 1,3PDO (Stevens et al, 2011), and in situ complex formation with semicarbazide, carbohydrazide or sodium bisulfite (Krauter et al, 2012;Rütti et al, 2011;Sardari et al, 2013;Stevens et al, 2013). Complexation of 3HPA with carbohydrazide has so far resulted in its production from high glycerol concentration at a quantitative molar yield approaching 1 mol/mol (Krauter et al, 2012).…”

“…The bound 3HPA could be eluted from the resin using saturated NaCl solution. However, when applied for in situ 3HPA removal, both the bisulfite (at a concentration exceeding 50.00 mM) and the anion exchanger led to severe repression of glycerol bioconversion (Rütti et al, 2011).…”

Semicarbazide-functionalized resin as a new scavenger for in situ recovery of 3-hydroxypropionaldehyde during biotransformation of glycerol by Lactobacillus reuteri

“…In contrast to Amberlite IRA-400 and free semicarbazide, the semicarbazidefunctionalized resin does not show any inhibitory effect on 3HPA production (Rütti et al, 2011). The production of 3HPA was increased as a result of reduced 3HPA toxicity to the biocatalyst, limited formation of by-products, and maintained a longer biocatalyst activity (data not shown).…”