Development of a recombinase polymerase amplification assay with lateral flow dipstick for rapid detection of feline parvovirus

Wang, Zhaohua; Wang, Xiao-Jia; Hou, Shaohua

doi:10.1016/j.jviromet.2019.113679

Cited by 16 publications

(11 citation statements)

References 29 publications

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“…Since there is no need to link the antibody and the oligonucleotide, the operation steps are simplified, and the versatility and convenience of this method are significantly higher than that of I-NAA. Currently, common NAA-I techniques include PCR-LFIA, − RPA-LFIA, , LAMP-LFIA, − and so on have been widely used in the field of testing.…”

Section: Immunoassay and Nucleic Acid Amplification Combined Technolo...mentioning

confidence: 99%

Nucleic Acid Amplification Techniques in Immunoassay: An Integrated Approach with Hybrid Performance

Sang

Cheng

et al. 2021

J. Agric. Food Chem.

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An immunoassay is mostly employed for the direct detection of food contaminants, and a molecular assay for targeting nucleic acids employs amplification techniques for distinguishing genes. The integration of an immunoassay with nucleic acid amplification techniques inherits the direct and rapid performance of an immunoassay and the ultrasensitive merit of a molecular assay. Enthusiastic attention has been attracted in recent years on the utilization of isothermal amplification techniques in an immunoassay, as well as the employment of a lateral flow immunoassay in a molecular assay. Thus, this Review discussed these kinds of approaches from two categories: immuno-nucleic acid amplification (I-NAA) and nucleic acid amplification-immunoassay (NAA-I). The advantages, drawbacks, and future developments were discussed for a comprehensive understanding.

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Section: Immunoassay and Nucleic Acid Amplification Combined Technolo...mentioning

confidence: 99%

Nucleic Acid Amplification Techniques in Immunoassay: An Integrated Approach with Hybrid Performance

Sang

Cheng

et al. 2021

J. Agric. Food Chem.

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“…Products of DNA amplification can be detected by a lateral flow dipstick (LFD), which makes the endpoint analysis more flexible and useable in low-resource settings (Crannell et al, 2014;Rostron et al, 2019;Hu et al, 2020;Lei et al, 2020). In previous studies, RPA combined with LFD (LFD-RPA) was found to save time and achieve high sensitivity, high specificity, and convenience, and visual detection of some diseases can be employed (Rosser et al, 2015;Poulton and Webster, 2018;Wang et al, 2019;Shelite et al, 2021). Sun et al set up the LFD-RPA method targeting SjR2 of S. japonicum to diagnose human S. japonicum infection by detecting faecal samples (Sun et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Development of a Recombinase Polymerase Amplification Assay for Schistosomiasis Japonica Diagnosis in the Experimental Mice and Domestic Goats

Guo

Zhou

Chen

et al. 2021

Front. Cell. Infect. Microbiol.

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Although the prevalence of schistosomiasis japonica has declined gradually in China, more accurate and sensitive diagnostic methods are urgently needed for the prevention and control of this disease. Molecular diagnostic methods are advantageous in terms of sensitivity and specificity, but they are time-consuming and require expensive instruments and skilled personnel, which limits their application in low-resource settings. In this study, an isothermal DNA amplification assay and recombinase polymerase amplification (RPA) combined with lateral flow dipstick (LFD) were set up. It was used to detect S. japonicum infections in experimental mice and domestic goats by amplifying a specific DNA fragment of S. japonicum. The lower limit of detection for the LFD-RPA assay was evaluated using dilutions of plasmid containing the target sequence. Cross-reactivity was evaluated using genomic DNA from eight other parasites. The effectiveness of the LFD-RPA assay was verified by assessing 36 positive plasma samples and 36 negative plasma samples from mice. The LFD-RPA assay and real-time PCR were also used to assess 48 schistosomiasis japonica-positive plasma samples and 53 negative plasma samples from goats. The LFD-RPA assay could detect 2.6 femtogram (fg) of S. japonicum target DNA (~39 fg genomic DNA of S. japonicum), only 10-fold less sensitive than real-time PCR assay. There was no cross-reactivity with DNA from the other eight parasites, such as Haemonchus contortus and Spirometra. The whole amplification process could be completed within 15 min at 39°C, and the results can be observed easily using the LFD. The sensitivity and specificity of the LFD-RPA assay were 97.22% (35/36, 95% CI, 85.47%–99.93%) and 100% (36/36, 95% CI, 90.26%–100%) in mice, and 93.75% (45/48, 95% CI, 82.80%–98.69%) and 100% (53/53, 95% CI, 93.28%–100%) in goats. By comparison, the sensitivity and specificity of real-time PCR were 100% (36/36, 95% CI, 90.26%–100%) and 100% (36/36, 95% CI, 90.26%–100%) for mice, and 97.92% (47/48, 95% CI, 88.93%–99.95%) and 100% (53/53, 95% CI, 93.28%–100%) for goats. The LFD-RPA assay exhibits high sensitivity and specificity for the diagnosis of schistosomiasis japonica, and it is an alternative method for diagnosis schistosomiasis japonica in low resource setting.

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“…The RPA assay has the advantages of rapidity, speci city, and sensitivity, and doesn't need expensive instruments [5,6]. After the RPA assay, lateral ow dipstick (LFD) could be used to detect the products of DNA ampli cation [7]. Recently, RPA assay coupled with LFD assay (RPA-LFD) has been reported to have a good potential in the detection of many pathogens, such as bovine ephemeral fever virus, African swine fever virus, and avian in uenza A virus [8][9][10].…”

Section: Introductionmentioning

confidence: 99%

Development of a reverse transcription recombinase polymerase amplification assay with lateral flow dipstick for rapid detection of classical swine fever virus

Fan¹,

Zhang²,

Chen³

et al. 2020

Preprint

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Background: Classical swine fever (CSF), caused by the infection of Classical swine fever virus (CSFV), is a highly contagious disease of pigs and has caused significant economic losses in the pig industry. The rapid and effective detection of CSFV would contribute to the eradication program against CSF. Thus, this study aimed to develop a rapid and simple method for CSFV detection.Results: Here, a new method based on reverse transcription recombinase polymerase amplification (RT-RPA) coupled with lateral flow dipstick (LFD) was established for detecting CSFV. The RPA assay could be completed within 20 min at 37oC and the results of the RPA assay could be visualized by LFD assay with the naked-eye inspection. This RT-RPA-LFD assay could be used to detect CSFV specifically, with no cross-reaction with other pathogens. Its detection limit was 10 pg of CSFV cDNA. Importantly, the RT-RPA-LFD assay has a good performance on the CSFV detection for clinical samples.Conclusions: The established RT-RPA-LFD assay greatly reduced the need for professional staff and sophisticated instruments and made disease detection convenient and feasible. This method would be useful for the prevention and control of CSF, especially in resource-limited settings.

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Development of a recombinase polymerase amplification assay with lateral flow dipstick for rapid detection of feline parvovirus

Cited by 16 publications

References 29 publications

Nucleic Acid Amplification Techniques in Immunoassay: An Integrated Approach with Hybrid Performance

Nucleic Acid Amplification Techniques in Immunoassay: An Integrated Approach with Hybrid Performance

Development of a Recombinase Polymerase Amplification Assay for Schistosomiasis Japonica Diagnosis in the Experimental Mice and Domestic Goats

Development of a reverse transcription recombinase polymerase amplification assay with lateral flow dipstick for rapid detection of classical swine fever virus

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